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Rabbit Recombinant Monoclonal p150 CAF1/CAF antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (AB232030), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (AB232030), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (AB232030), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (AB232030), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (AB232030), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)ICC/IFIHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.

Alternative names

Chromatin assembly factor 1 subunit A, CAF-1 subunit A, Chromatin assembly factor I p150 subunit, CAF-I 150 kDa subunit, CAF-I p150, hp150, CAF1P150, CAF, CHAF1A

Recommended products

Rabbit Recombinant Monoclonal p150 CAF1/CAF antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR5576(2)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab232030 is the carrier-free version of Anti-p150 CAF1/CAF antibody [EPR5576(2)] ab126625.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The p150 CAF1 also known as CAF protein and CAF1 protein is part of the chromatin assembly factor 1 complex. It weighs approximately 150 kDa. This protein primarily expresses in the nucleus of eukaryotic cells where it plays an important role in DNA replication and repair. The p150 protein acts as a structural component that is essential for proper chromatin assembly during the S phase of the cell cycle.

Biological function summary

This target functions as part of the CAF-1 complex which includes two additional subunits—p60 and p48. The complex aids in the assembly of newly synthesized histones onto replicating DNA facilitating nucleosome formation. It affects chromatin dynamics influencing transcriptional regulation by providing nucleosome assembly binding sites along DNA. This process maintains genomic integrity during cell division and influences gene expression in response to various cell signals.

Pathways

The p150 protein interacts within pathways involved in DNA replication and repair. It plays a major role in the nucleosome assembly pathway closely interacting with histone proteins H3 and H4. Through these interactions it ensures the stability of replication forks during DNA synthesis. Besides it participates in the regulation of the cell cycle and influences the chromatin assembly pathway important for accurate DNA replication and cell division.

Associated diseases and disorders

Several conditions show connection with the p150 protein's function in DNA replication. Alterations or defects in its expression link to cancer where chromatin misassembly leads to genomic instability a hallmark of tumorigenesis. Additionally the involvement of p150 in neurodegenerative disorders sheds light on how disrupted chromatin assembly can affect neural cells. Its interaction with proteins like retinoblastoma (Rb) points to the potential dysregulation in cell cycle control further influencing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030), expandable thumbnail

    Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030)

    All lanes: Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (Anti-p150 CAF1/CAF antibody [EPR5576(2)] ab126625) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) starved 24 hours and treated with 500ng/ml nocodazole for 24 hours whole cell lysate

    Lane 3: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    Lane 4: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa

  • Flow Cytometry (Intracellular) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030)

    This data was developed using ab232030, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling p150 CAF1/CAF with Purified ab232030 at 1:20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030)

    This data was developed using Anti-p150 CAF1/CAF antibody [EPR5576(2)] ab126625, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling p150 CAF1/CAF with Purified Anti-p150 CAF1/CAF antibody [EPR5576(2)] ab126625 at 1:150 (0.9 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030)

    This data was developed using ab232030, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling p150 CAF1/CAF with Purified ab232030 at 1:50 dilution (2.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] - BSA and Azide free (ab232030)

    This data was developed using Anti-p150 CAF1/CAF antibody [EPR5576(2)] ab126625, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling p150 CAF1/CAF with Purified Anti-p150 CAF1/CAF antibody [EPR5576(2)] ab126625 at 1:150 (0.9 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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