JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB88304

Anti-P15RS antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Polyclonal P15RS antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RPRD1A.

View Alternative Names

P15RS, RPRD1A, Regulation of nuclear pre-mRNA domain-containing protein 1A, Cyclin-dependent kinase inhibitor 2B-related protein, p15INK4B-related protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-P15RS antibody (AB88304)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-P15RS antibody (AB88304)

ab88304, at 10 µg/ml, staining P15RS in HeLa cell by Immunofluorescence.

Western blot - Anti-P15RS antibody (AB88304)
  • WB

Unknown

Western blot - Anti-P15RS antibody (AB88304)

All lanes:

Western blot - Anti-P15RS antibody (ab88304) at 1 µg/mL

Lane 1:

P15RS transfected 293T cell lysate at 50 µg

Lane 2:

Non transfected 293T cell lysate at 50 µg

Predicted band size: 36 kDa

Observed band size: 32 kDa

false

Western blot - Anti-P15RS antibody (AB88304)
  • WB

Unknown

Western blot - Anti-P15RS antibody (AB88304)

All lanes:

Western blot - Anti-P15RS antibody (ab88304) at 1 µg/mL

All lanes:

human liver tissue lysate at 50 µg

Predicted band size: 36 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RPRD1A.

Q96P16

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P15RS also known as CDKN2B-AS1 is a non-coding RNA with a molecular weight of approximately 24 kDa. P15RS functions as a regulator of gene expression and influences various cellular activities. It is expressed in many tissues with notable abundance in the brain heart and skeletal muscles. P15RS plays an important mechanical role in modulating cell cycle progression and can contribute to cellular response to different stimuli.
Biological function summary

P15RS regulates the interaction of proteins involved in apoptosis and cell survival serving as a modulator between these processes. It associates with complexes that control cell cycle transitions particularly at the G1/S checkpoint. This RNA interacts directly with proteins such as cyclin-dependent kinases affecting their activity and contributing to the balance between cell proliferation and arrest.

Pathways

This regulatory RNA operates within the p53 signaling pathway and participates in the control of cellular aging and apoptosis. It interacts with proteins like p21 and p27 which are key regulators in these processes. Through these interactions P15RS helps maintain the integrity of cellular mechanisms that govern cell growth and response to DNA damage playing a role in preventing unscheduled progression in the cell cycle.

The dysregulation of P15RS expression has been implicated in cancer especially in correlating with tumor suppressive activity. Alterations in its function can lead to uncontrolled cell proliferation seen in malignancies. In addition to cancer P15RS has associations with cardiovascular diseases where its modulation affects cellular survival under stress conditions. Its interaction with p16 another tumor suppressor highlights its role in maintaining normal cell cycle regulation and potential for its consideration in therapeutic targets.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. May act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle.
See full target information RPRD1A
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 306:C639-47 PubMed24401845

2014

Proteomic mapping of proteins released during necrosis and apoptosis from cultured neonatal cardiac myocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kurt D Marshall,Michelle A Edwards,Maike Krenz,J Wade Davis,Christopher P Baines
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB151992

Anti-ABCB7 antibody

Western blot - Anti-ABCB7 antibody (AB151992)

  • 0
  • 0
Primary Antibodies

AB81299

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB81299)

  • 4
  • 17
Primary Antibodies

AB197979

Anti-CD161 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody (AB197979)

  • 4
  • 3
Primary Antibodies

AB133645

Anti-67kDa Laminin Receptor antibody [EPR8469]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-67kDa Laminin Receptor antibody [EPR8469] (AB133645)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com