Rabbit Recombinant Monoclonal p18 INK4c/CDKN2C antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 16 publications.
Images
![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (AB192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--western-blot-img140337.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (AB192239)")
![Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (AB192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--immunoprecipitation-img98234.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (AB192239)")
![Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (AB192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--immunocytochemistry-immunofluorescence-img66379.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (AB192239)")
![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (AB192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--western-blot-img71000.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (AB192239)")
Publications
Filter by
- International journal of molecular sciences 24:2023Large HBV Surface Protein-Induced Unfolded Protein Response Dynamically Regulates p27 Degradation in Hepatocellular Carcinoma Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixiao Guo,Jie Shao,Renyu Zhang,Mingwei Han,Lingmin Kong,Zekun Liu,Hao Li,Ding Wei,Meng Lu,Shuai Zhang,Cong Zhang,Haolin Wei,Zhinan Chen,Huijie BianPubMed 37762128
- Cancer cell international 22:3822022Identification and validation of an E2F-related gene signature for predicting recurrence-free survival in human prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Yang,Lei Chen,Qingsong Niu,Qintao Ge,Jiong Zhang,Junyue Tao,Jun Zhou,Chaozhao LiangPubMed 36471446
- Neural regeneration research 18:609-6172022Small extracellular vesicles secreted by induced pluripotent stem cell-derived mesenchymal stem cells improve postoperative cognitive dysfunction in mice with diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Li Lang,Yan-Zhi Zhao,Ren-Jie Xiao,Jing Sun,Yong Chen,Guo-Wen Hu,Guo-Hai XuPubMed 36018185
- Bioscience reports 42:2022INK4 cyclin-dependent kinase inhibitors as potential prognostic biomarkers and therapeutic targets in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Liu,Songhao Jia,Kun Guo,Rongkuan LiPubMed 35771229
- BMC pulmonary medicine 22:2462022Clinical significance of cyclin-dependent kinase inhibitor 2C expression in cancers: from small cell lung carcinoma to pan-cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuo-Sheng Li,Gang Chen,Jun Liu,Deng Tang,Jin-Hua Zheng,Jing Luo,Mei-Hua Jin,Hua-Song Lu,Chong-Xi Bao,Jia Tian,Wu-Sheng Deng,Jing-Wei Fu,Yue Feng,Neng-Yong Zeng,Hua-Fu Zhou,Jin-Liang KongPubMed 35751045
- Translational research : the journal of laboratory and clinical medicine 242:105-1212021CDKN2C expression in adipose tissue is reduced in type II diabetes and central obesity: impact on adipocyte differentiation and lipid storage?Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria J Pereira,Milica Vranic,Prasad G Kamble,Henning Jernow,Robin Kristófi,Ema Holbikova,Stanko Skrtic,Joel Kullberg,Maria K Svensson,Susanne Hetty,Jan W ErikssonPubMed 34896253
- NPJ precision oncology 5:142021Stabilization of p18 by deubiquitylase CYLD is pivotal for cell cycle progression and viral replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYueshuo Li,Feng Shi,Jianmin Hu,Longlong Xie,Lin Zhao,Min Tang,Xiangjian Luo,Mao Ye,Hui Zheng,Min Zhou,Na Liu,Ann M Bode,Jia Fan,Jian Zhou,Qiang Gao,Shuangjian Qiu,Weizhong Wu,Xin Zhang,Weihua Liao,Ya CaoPubMed 33654169
- Journal of cellular and molecular medicine 25:1896-19102021miR-34a in extracellular vesicles from bone marrow mesenchymal stem cells reduces rheumatoid arthritis inflammation via the cyclin I/ATM/ATR/p53 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuaiguo Wu,Xike Zhou,Xuedong Wang,Wei Cheng,Xinjia Hu,Yueping Wang,Bing Luo,Wenjun Huang,Juan GuPubMed 33465281
- Cellular and molecular life sciences : CMLS 78:3485-35012020Kidney inflammaging is promoted by CCR2 macrophages and tissue-derived micro-environmental factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLise Lefèvre,Jason S Iacovoni,Hélène Martini,Julie Bellière,Damien Maggiorani,Marianne Dutaur,Dimitri J Marsal,Pauline Decaunes,Nathalie Pizzinat,Jeanne Mialet-Perez,Daniel Cussac,Angelo Parini,Victorine Douin-EchinardPubMed 33313981
- Nature communications 11:27072020A genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarla Eller et. al.PubMed 32483149
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
Alternative names
CDKN6, CDKN2C, Cyclin-dependent kinase 4 inhibitor C, Cyclin-dependent kinase 6 inhibitor, p18-INK4c, p18-INK6
Recommended products
Rabbit Recombinant Monoclonal p18 INK4c/CDKN2C antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 16 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15891
- Purification technique
- Ion exchange chromatography
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P18 INK4c also known as CDKN2C is a cyclin-dependent kinase inhibitor that plays an important role in cell cycle regulation. It is a member of the INK4 family and carries a molecular mass of approximately 18 kDa. This protein actively inhibits the activity of CDK4 and CDK6 key kinases that drive the transition from the G1 to S phase in the cell cycle. p18 INK4c is expressed in various tissues with notable expression in the adrenal gland kidney and the nervous system.
- Biological function summary
P18 INK4c functions as a regulator by acting as a part of the complex that controls cell proliferation. It ensures that cells do not proceed through the G1/S checkpoint inappropriately maintaining proper cell cycle control. This protein serves as a brake in the cell cycle by binding to CDK4/6 thereby preventing their interaction with D-type cyclins and subsequent cell cycle progression.
- Pathways
P18 INK4c is an important component of the retinoblastoma (Rb) pathway as well as the p16INK4a-pRB pathway. These pathways are important for negative regulation of the cell cycle. In these pathways p18 INK4c inhibits CDK4 and CDK6 which are kinases needed to phosphorylate the Rb protein a pivotal step for cell cycle progression. The regulation of these pathways plays a part in processes like cellular senescence and differentiation.
- Associated diseases and disorders
P18 INK4c is associated with various types of cancer including glioma and breast cancer. Alterations in the expression or function of p18 INK4c can lead to unchecked cell proliferation and tumorigenesis. It often interacts with proteins such as CDK4 and CDK6 in these contexts where dysregulation may contribute to the development and progression of the diseases. Understanding p18 INK4c's role could provide insights into potential therapeutic strategies for these cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--western-blot-img140337.jpg "Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
Lanes 1-4: Merged signal (red and green). Green - ab192239 observed at 18 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab192239 Anti-p18 INK4c/CDKN2C antibody [EPR15891] was shown to specifically react with Cyclin Dependent Kinase Inhibitor 2C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265031 (knockout cell lysate ab257887) was used. Wild-type and Cyclin Dependent Kinase Inhibitor 2C knockout samples were subjected to SDS-PAGE. ab192239 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: CDKN2C knockout HeLa cell lysate at 20 µg
Lane 3: Rat kidney cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 18 kDa
![Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--immunoprecipitation-img98234.jpg "Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
Western blot analysis of immunoprecipitation pellet from HeLa lysate immunoprecipitated using ab192239 at 1/30 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.All lanes: Immunoprecipitation - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
Predicted band size: 18 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--immunocytochemistry-immunofluorescence-img66379.jpg "Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Immunocytochemistry/ Immunofluorescence - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling p18 INK4C/CDKN2C with ab192239 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-p18 INK4C/CDKN2C at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--western-blot-img71000.jpg "Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
All lanes: Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/1000 dilution
Lane 1: Rat kidney lysate at 10 µg
Lane 2: Rat spleen lysate at 10 µg
Lane 3: NIH 3T3 lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 18 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136578.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--western-blot-img105692.jpg "Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
All lanes: Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239) at 1/10000 dilution
Lane 1: 293 cell lysate at 20 µg
Lane 2: Ramos cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 18 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137942.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling p18 INK4C/CDKN2C with ab192239 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239), expandable thumbnail](https://content.abcam.com/products/images/p18-ink4c-cdkn2c-antibody-epr15891-ab192239--western-blot-img105692.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p18 INK4c/CDKN2C antibody [EPR15891] (ab192239)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com