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AB227443

Anti-p21 antibody

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(1 Review)

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(37 Publications)

Rabbit Polyclonal p21 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 37 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDKN1A.

View Alternative Names

CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, CDKN1A, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21, MDA-6

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody (AB227443)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody (AB227443)

Paraffin-embedded SkHep1 xenograft tissue stained for p21 using ab227443 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-p21 antibody (AB227443)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p21 antibody (AB227443)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with 30 μM cisplatin for 24 hours, stained for p21 (green) using ab227443 at 1/500 dilution in ICC/IF. Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody (AB227443)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody (AB227443)

Immunohistochemical analysis of paraffin-embedded human normal colon tissue stained for p21 Cip1 protein at nucleus with ab227443 at a 1/500 dilution.

Antigen retrieval : EDTA buffer, 20 min

Western blot - Anti-p21 antibody (AB227443)
  • WB

Supplier Data

Western blot - Anti-p21 antibody (AB227443)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-p21 antibody (ab227443) at 1/1000 dilution

Lane 1:

HCT116 whole cell lysate at 30 µg

Lane 2:

HCT116 treated with 30uM cisplatin for 24hr cell lysate at 30 µg

Secondary

All lanes:

Western blot

Predicted band size: 18 kDa

false

Western blot - Anti-p21 antibody (AB227443)
  • WB

Supplier Data

Western blot - Anti-p21 antibody (AB227443)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-p21 antibody (ab227443) at 1/500 dilution

Lane 1:

Wild-type p21 HeLa cell extracts at 30 µg

Lane 2:

p21 Cip1 knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 18 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CDKN1A. The exact immunogen used to generate this antibody is proprietary information.

P38936

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p21 protein also known as CDKN1A is an important regulator of cell cycle progression. It acts as a cyclin-dependent kinase inhibitor where it binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes. The molecular weight of p21 is approximately 21 kDa. Cellular expression of p21 is diverse; it occurs in various tissues reflecting its broad role in maintaining cell cycle control. This protein's expression level is often assessed using techniques such as p21 western blotting p21 ELISA and p21 IHC staining.
Biological function summary

P21 plays a critical role in cell cycle regulation and DNA damage response. It associates with the p53 tumor suppressor protein forming an essential part of the p53 signaling pathway under stress conditions. p21 arrests cells in G1 phase allowing DNA repair or leading to cellular senescence. Through these mechanisms p21 integrates signals from stress and damage emphasizing its importance as a mediator in cellular checkpoint control.

Pathways

P21 is closely involved in the p53 and the Transforming Growth Factor-beta (TGF-β) signaling pathways. This integration helps in transmitting growth-inhibitory signals. p21 interacts with proteins such as p53 and cyclins ensuring precise regulation of the cell cycle and growth arrest when necessary. Triggered by p53 activation p21 contributes to preventing undisturbed cell proliferation acting as a safeguard against tumor formation.

P21's deregulation associates with cancer and age-related diseases. Its impairment is a common feature in many cancers often linked with defects in the p53 pathway. Reduced p21 expression can contribute to unchecked cell division and tumor progression. Moreover in age-related diseases p21's role in promoting cellular senescence connects it with degenerative conditions. The intricate relationship between p21 and proteins such as p53 and cyclins highlights its potential as a therapeutic target for these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed : 9106657). Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (By similarity). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed : 11595739). Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (By similarity).
See full target information CDKN1A
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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Nature aging 5:219-236 PubMed39730825

2024

A ganglioside-based immune checkpoint enables senescent cells to evade immunosurveillance during aging.

Applications

Unspecified application

Species

Unspecified reactive species

Charlène Iltis,Iryna Moskalevska,Antoine Debiesse,Laetitia Seguin,Christina Fissoun,Ludovic Cervera,Lyvia Moudombi,Maude Ardin,Anthony Ferrari,Coline Eliott,Didier Pisani,Alexandre Ottaviani,Manon Bourinet,Carmelo Luci,Philippe Gual,Gabriela Makulyte,David Bernard,Manon Durandy,Lou C Duret,Tynhinane Hamidouche,Sarah Kunz,Olivier Croce,Clément Delannoy,Yann Guérardel,Fabrice Allain,Paul Hofman,Delphine Benarroch-Popivker,Laurence Bianchini,Berengère Dadone-Montaudie,Estelle Cosson,Julien Guglielmi,Thierry Pourcher,Véronique M Braud,Marina Shkreli,Yves-Marie Pers,Christian Jorgensen,Jean-Marc Brondello,Chloé C Féral,Marie-Cécile Michallet,Eric Gilson,Julien Cherfils-Vicini

Biochemical genetics : PubMed39540959

2024

The LINC01094/miR-545-3p/SLC7A11 Signaling Axis Promotes the Development of Gastric Cancer by Regulating Cell Growth and Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Chao Li,Song Meng,Yu-Ting Kuang

Nanomedicine (London, England) 19:2159-2170 PubMed39229808

2024

Nanoparticle-delivered quercetin exhibits enhanced efficacy in eliminating iron-overloaded senescent chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Asima Karim,Rizwan Qaisar,Savitha Suresh,Jayalakshmi Jagal,Mutasem Rawas-Qalaji

Aging cell 23:e14325 PubMed39185703

2024

Pathogenic TDP-43 accelerates the generation of toxic exon1 HTT in Huntington's disease knock-in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dazhang Bai,Fuyu Deng,Qingqing Jia,Kaili Ou,Xiang Wang,Junqi Hou,Longhong Zhu,Mingwei Guo,Su Yang,Guohui Jiang,Shihua Li,Xiao-Jiang Li,Peng Yin

Bioactive materials 32:277-291 PubMed37876556

2023

Engineered a dual-targeting HA-TPP/A nanoparticle for combination therapy against co-mutation in gastrointestinal cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Mei,Xiaohua Qin,Zhenyu Yang,Shiyao Song,Xiaoting Liu,Chong Wu,Jieying Qian,Xiaowan Huang,Yunjiao Zhang,Weiling He

BMC medical genomics 16:218 PubMed37710236

2023

Uncovering the ceRNA network and DNA methylation associated with gene expression in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhang,Lu Pei,Wen-Li Qiu,Yu-Xia Wei,Bi-Yun Liao,Feng-Lian Yang

American journal of cancer research 13:2323-2341 PubMed37424824

2023

USP37 promotes angiogenesis and metastasis in colorectal cancer by facilitating β-catenin stability.

Applications

Unspecified application

Species

Unspecified reactive species

Dafeng Tong,Jie Yuan,Zhaoming Wang,Enda Yu,Jifu E

Journal of experimental & clinical cancer research : CR 42:7 PubMed36604676

2023

BETi enhance ATGL expression and its lipase activity to exert their antitumoral effects in triple-negative breast cancer (TNBC) cells.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Rossi,Raffaella Zamponi,Mattea Chirico,Maria Elena Pisanu,Egidio Iorio,Federica Torricelli,Mila Gugnoni,Alessia Ciarrocchi,Mariaelena Pistoni

Neoplasma 69:1386-1395 PubMed36591801

2023

GDF11 knockdown downregulates SMURF1 to inhibit breast cancer progression by activation of p53 and inactivation of ERα signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Li Tang,Qi Wang,Jian-Guo Lu,Xiao-Jun Yang,Jiao-Jiao Shi,Sheng-Peng Wang,Chang-Qing Cao,Hua-Dong Zhao

EMBO reports 24:e55503 PubMed36440617

2022

TRAPPC1 is essential for the maintenance and differentiation of common myeloid progenitors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Xu,Zhaoqi Zhang,Yang Zhao,Chenxu Zhao,Mingpu Shi,Xue Dong,Jiayu Zhang,Liang Tan,Lianfeng Zhang,Yong Zhao
View all publications
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