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Mouse Monoclonal p21 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDKN1A.

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Images

Immunocytochemistry - Anti-p21 antibody [CIP1/823] (AB220206), expandable thumbnail
  • Western blot - Anti-p21 antibody [CIP1/823] (AB220206), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] (AB220206), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] (AB220206), expandable thumbnail
  • Western blot - Anti-p21 antibody [CIP1/823] (AB220206), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human CDKN1A. Database link P38936

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCWB
Human
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
2-4 µg/mL
Notes

(Primary incubation for 30 minutes at RT).

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

(Primary incubation for 30 minutes at RT).

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

(Primary incubation for 30 minutes at RT).

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed:9106657). Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (By similarity). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739). Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (By similarity).

Alternative names

CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, CDKN1A, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21, MDA-6

Recommended products

Mouse Monoclonal p21 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDKN1A.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human CDKN1A. Database link P38936
Clone number
CIP1/823
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...
Purification notes

ab220206 was purified from Bioreactor Concentrate by Protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The p21 protein also known as CDKN1A is an important regulator of cell cycle progression. It acts as a cyclin-dependent kinase inhibitor where it binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes. The molecular weight of p21 is approximately 21 kDa. Cellular expression of p21 is diverse; it occurs in various tissues reflecting its broad role in maintaining cell cycle control. This protein's expression level is often assessed using techniques such as p21 western blotting p21 ELISA and p21 IHC staining.

Biological function summary

P21 plays a critical role in cell cycle regulation and DNA damage response. It associates with the p53 tumor suppressor protein forming an essential part of the p53 signaling pathway under stress conditions. p21 arrests cells in G1 phase allowing DNA repair or leading to cellular senescence. Through these mechanisms p21 integrates signals from stress and damage emphasizing its importance as a mediator in cellular checkpoint control.

Pathways

P21 is closely involved in the p53 and the Transforming Growth Factor-beta (TGF-β) signaling pathways. This integration helps in transmitting growth-inhibitory signals. p21 interacts with proteins such as p53 and cyclins ensuring precise regulation of the cell cycle and growth arrest when necessary. Triggered by p53 activation p21 contributes to preventing undisturbed cell proliferation acting as a safeguard against tumor formation.

Associated diseases and disorders

P21's deregulation associates with cancer and age-related diseases. Its impairment is a common feature in many cancers often linked with defects in the p53 pathway. Reduced p21 expression can contribute to unchecked cell division and tumor progression. Moreover in age-related diseases p21's role in promoting cellular senescence connects it with degenerative conditions. The intricate relationship between p21 and proteins such as p53 and cyclins highlights its potential as a therapeutic target for these disorders.

Product promise

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5 product images

  • Immunocytochemistry - Anti-p21 antibody [CIP1/823] (ab220206), expandable thumbnail

    Immunocytochemistry - Anti-p21 antibody [CIP1/823] (ab220206)

    Immunocytochemistry analysis of HeLa cells labeling p21 with ab220206 at 1 ug/ml followed by Goat anti-Mouse IgG (Red). Membrane stained with Phalloidin 488 (Green).

  • Western blot - Anti-p21 antibody [CIP1/823] (ab220206), expandable thumbnail

    Western blot - Anti-p21 antibody [CIP1/823] (ab220206)

    Western blot analysis of HeLa cell lysate labeling p21 with ab220206 at 1 ug/ml.

    All lanes: Western blot - Anti-p21 antibody [CIP1/823] (ab220206) at 1 µg/mL

    All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] (ab220206), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] (ab220206)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling p21 with ab220206 at 4 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] (ab220206), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] (ab220206)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue labeling p21 with ab220206 at 4 μg/ml.

  • Western blot - Anti-p21 antibody [CIP1/823] (ab220206), expandable thumbnail

    Western blot - Anti-p21 antibody [CIP1/823] (ab220206)

    Western blot: Anti-CDKN1A antibody [CIP1/823] (ab220206) staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab220206 was shown to bind specifically to CDKN1A. A band was observed at 21 kDa in wild-type cell lysates with no signal observed at this size in CDKN1A knockout cell lines. To generate this image, wild-type and CDKN1A knockout cell lysates were analysed. CDKN1A knockout cell lines in HCT 116 (Human CDKN1A knockout HCT116 cell line ab288187), MCF7 (Human CDKN1A knockout MCF7 cell line ab288200) and A549 (Human CDKN1A knockout A549 cell line ab288213) backgrounds were used. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-p21 antibody [CIP1/823] (ab220206) at 1 µg/mL

    Lane 1: Wild-type HCT 116 cell lysate at 20 µg

    Lane 2: Western blot - Human CDKN1A knockout HCT116 cell line (Human CDKN1A knockout HCT116 cell line ab288187)

    Lane 2: CDKN1A knockout HCT 116 cell lysate at 20 µg

    Lane 3: Wild-type MCF7 cell lysate at 20 µg

    Lane 4: Western blot - Human CDKN1A knockout MCF7 cell line (Human CDKN1A knockout MCF7 cell line ab288200)

    Lane 4: CDKN1A knockout MCF7 cell lysate at 20 µg

    Lane 5: Wild-type A549 cell lysate at 20 µg

    Lane 6: Western blot - Human CDKN1A knockout A549 cell line (Human CDKN1A knockout A549 cell line ab288213)

    Lane 6: CDKN1A knockout A549 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 18 kDa

    Observed band size: 21 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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