Anti-p21 antibody [CIP1/823]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] (AB220206)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue labeling p21 with ab220206 at 4 μg/ml.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] (AB220206)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling p21 with ab220206 at 4 μg/ml.

• ICC

Supplier Data

Immunocytochemistry - Anti-p21 antibody [CIP1/823] (AB220206)

Immunocytochemistry analysis of HeLa cells labeling p21 with ab220206 at 1 ug/ml followed by Goat anti-Mouse IgG (Red). Membrane stained with Phalloidin 488 (Green).

• WB

Supplier Data

Western blot - Anti-p21 antibody [CIP1/823] (AB220206)

Western blot analysis of HeLa cell lysate labeling p21 with ab220206 at 1 ug/ml.

All lanes:

Western blot - Anti-p21 antibody [CIP1/823] (ab220206) at 1 µg/mL

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 18 kDa

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• WB

Lab

Western blot - Anti-p21 antibody [CIP1/823] (AB220206)

Western blot : Anti-CDKN1A antibody [CIP1/823] (ab220206) staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab220206 was shown to bind specifically to CDKN1A. A band was observed at 21 kDa in wild-type cell lysates with no signal observed at this size in CDKN1A knockout cell lines. To generate this image, wild-type and CDKN1A knockout cell lysates were analysed. CDKN1A knockout cell lines in HCT 116 (ab288187), MCF7 (ab288200) and A549 (ab288213) backgrounds were used. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-p21 antibody [CIP1/823] (ab220206) at 1 µg/mL

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

Western blot - Human CDKN1A knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-cdkn1a-knockout-hct116-cell-line-ab288187'>ab288187</a>)

Lane 2:

CDKN1A knockout HCT 116 cell lysate at 20 µg

Lane 3:

Wild-type MCF7 cell lysate at 20 µg

Lane 4:

Western blot - Human CDKN1A knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-cdkn1a-knockout-mcf7-cell-line-ab288200'>ab288200</a>)

Lane 4:

CDKN1A knockout MCF7 cell lysate at 20 µg

Lane 5:

Wild-type A549 cell lysate at 20 µg

Lane 6:

Western blot - Human CDKN1A knockout A549 cell line (<a href='/en-us/products/cell-lines/human-cdkn1a-knockout-a549-cell-line-ab288213'>ab288213</a>)

Lane 6:

CDKN1A knockout A549 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 21 kDa

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