Anti-p21 antibody [CIP1/823] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] - BSA and Azide free (AB212247)

Immunohistochemical analysis of formalix-fixed paraffin-embedded Human lung squamous cell carcinoma tissue, labeling p21 using ab212247 at 4 μg/mL.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] - BSA and Azide free (AB212247)

Immunohistochemical analysis of formalix-fixed paraffin-embedded Human bladder carcinoma tissue, labeling p21 using ab212247 at 4 μg/mL.

• ICC

Supplier Data

Immunocytochemistry - Anti-p21 antibody [CIP1/823] - BSA and Azide free (AB212247)

Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling p21 with ab212247 followed by Goat anti-Mouse IgG-CF555 (Red). Membrane stained with Phalloidin 488 (Green).

• WB

Supplier Data

Western blot - Anti-p21 antibody [CIP1/823] - BSA and Azide free (AB212247)

All lanes:

Western blot - Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 18 kDa

Observed band size: 21 kDa

false

• WB

Lab

Western blot - Anti-p21 antibody [CIP1/823] - BSA and Azide free (AB212247)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220206). Western blot : Anti-CDKN1A antibody [CIP1/823] (ab220206) staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab220206 was shown to bind specifically to CDKN1A. A band was observed at 21 kDa in wild-type cell lysates with no signal observed at this size in CDKN1A knockout cell lines. To generate this image, wild-type and CDKN1A knockout cell lysates were analysed. CDKN1A knockout cell lines in HCT 116 (ab288187), MCF7 (ab288200) and A549 (ab288213) backgrounds were used. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-p21 antibody [CIP1/823] (<a href='/en-us/products/primary-antibodies/p21-antibody-cip1-823-ab220206'>ab220206</a>) at 1 µg/mL

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

Western blot - Human CDKN1A knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-cdkn1a-knockout-hct116-cell-line-ab288187'>ab288187</a>)

Lane 2:

CDKN1A knockout HCT 116 cell lysate at 20 µg

Lane 3:

Wild-type MCF7 cell lysate at 20 µg

Lane 4:

Western blot - Human CDKN1A knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-cdkn1a-knockout-mcf7-cell-line-ab288200'>ab288200</a>)

Lane 4:

CDKN1A knockout MCF7 cell lysate at 20 µg

Lane 5:

Wild-type A549 cell lysate at 20 µg

Lane 6:

Western blot - Human CDKN1A knockout A549 cell line (<a href='/en-us/products/cell-lines/human-cdkn1a-knockout-a549-cell-line-ab288213'>ab288213</a>)

Lane 6:

CDKN1A knockout A549 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 21 kDa

false