Anti-p21 antibody [HUGO291]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (AB107099)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hek-V5-mp21 transfected cells labelling p21 with ab107099. Nuclear staining was observed in Hek-V5-mp21 cytospins. Labeling with the anti-V5 mAb confirmed the efficiency of transfection. Cytospin preparation of human CD8 was used as a negative control. This image was from a different batch produced using hybridoma method.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (AB107099)

Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling p21 with ab107099 at 1/100 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Scattered staining on mouse lung cancer.
The section was incubated with ab107099 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (AB107099)

Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling p21 with ab107099 at 1/100 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse breast cancer.
The section was incubated with ab107099 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [HUGO291] (AB107099)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse p21WT fibrosarcoma and mouse p21KO fibrosarcoma tissue sections labeling p21 with ab107099.

This image was from a different batch produced using hybridoma method.

• WB

Lab

Western blot - Anti-p21 antibody [HUGO291] (AB107099)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

ab181602 was used as a GAPDH loading control at a 1/200000 dilution.

The expression of p21 is upregulated in response to doxorubicin and Nutlin3 treatment (PMID : 15471885 and PMID : 19648117).
This blot was developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-p21 antibody [HUGO291] (ab107099) at 1/1000 dilution

Lane 1:

Untreated MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Lane 2:

MEF treated with 10uM doxorubicin (<a href='/en-us/products/biochemicals/doxorubicin-hydrochloride-topoisomerase-ii-inhibitor-ab120629'>ab120629</a>) for 16 hours(+) whole cell lysate at 20 µg

Lane 3:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 treated with 10uM Nutlin3 (<a href='/en-us/products/biochemicals/nutlin-3-mdm2-antagonist-ab120646'>ab120646</a>) for 24 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Observed band size: 17 kDa

true

Exposure time: 92s

• WB

Lab

Western blot - Anti-p21 antibody [HUGO291] (AB107099)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-p21 antibody [HUGO291] (ab107099) at 1/1000 dilution

All lanes:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Observed band size: 17 kDa

true

Exposure time: 92s

• WB

Unknown

Western blot - Anti-p21 antibody [HUGO291] (AB107099)

This image was from a different batch produced using hybridoma method.

All lanes:

Western blot - Anti-p21 antibody [HUGO291] (ab107099) at 1/50 dilution

Lane 1:

TT243 MEFs (non-irradiated) at 18.83 µg

Lane 2:

TT243 MEFs (irradiated with 10 Gy) at 20.8 µg

Lane 3:

p21-/- KO MEFs at 60 µg

Lane 4:

P21 WT MEFs at 75 µg

Lane 5:

HEK-V5-mp21 transfected cells at 20 µg

Lane 6:

HEK-V5-mp15 transfected cells at 20 µg

Predicted band size: 18 kDa

false