AB37916

Anti-p21-ARC/ARPC3 antibody

Name: Anti-p21-ARC/ARPC3 antibody (ab37916) | Abcam
Brand: Abcam Limited
SKU: AB37916
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Goat Polyclonal p21-ARC/ARPC3 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

ARC21, ARPC3, Actin-related protein 2/3 complex subunit 3, Arp2/3 complex 21 kDa subunit, p21-ARC

1 Images

Unknown

Western blot - Anti-p21-ARC/ARPC3 antibody (AB37916)

Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-p21-ARC/ARPC3 antibody (ab37916) at 1 µg/mL

All lanes:

Mouse Brain lysate (RIPA buffer 35µg total protein per lane).

Predicted band size: 21 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p21-ARC also known as ARPC3 is a subunit of the actin-related protein 2/3 complex (Arp2/3 complex). This protein plays a critical role in the nucleation of new actin filaments promoting the formation of branched actin networks. ARPC3 has a molecular mass of approximately 21 kDa and its expression can be found in a variety of tissues including the heart and kidney. The protein operates in the cytoplasm where it associates with other proteins in the Arp2/3 complex to mediate its function.

Biological function summary

The p21-ARC/ARPC3 is an essential component of the highly conserved Arp2/3 complex involved in actin filament assembly and organization. This complex binds to the sides of pre-existing actin filaments and nucleates new ones creating branching and forming web-like structures needed for various cellular processes. The Arp2/3 complex including ARPC3 is involved in cell movement endocytosis and intracellular trafficking. This protein's activity enhances the dynamic restructuring of the actin cytoskeleton necessary for these functions.

Pathways

ARPC3 influences pathways related to cell motility and cytoskeletal reorganization. It participates in the Wiskott-Aldrich Syndrome Protein (WASP) pathway where it acts alongside proteins such as WASP and the WAVE complex to regulate actin polymerization. The process converts signals from the cell surface into mechanical force driving the extension of pseudopodia and lamellipodia. Another important pathway involves the Rho family GTPases where ARPC3 links to actin dynamics cell polarity and the orchestration of actin assembly in response to signaling events within the cell.

Abnormalities in ARPC3 function or expression can influence neurological and immunological conditions. For instance dysregulation of the Arp2/3 complex can enhance susceptibility to diseases like Wiskott-Aldrich syndrome which is tied to impaired signaling and cytoskeletal rearrangement. ARPC3 interacts with proteins related to immune response such as WASP making it significant in the context of immune deficiency disorders. Furthermore mutations affecting actin cytoskeleton dynamics can lead to neurodegenerative diseases highlighting the important impact of ARPC3 and associated Arp2/3 complex proteins in maintaining cellular function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).

See full target information ARPC3

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 46:650-662 PubMed38248344

2024

Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaozhen Zhou,Qianqian Zhang,Lingzi Liao,Qian Li,Huidan Qu,Xinyu Wang,Ying Zhou,Guangzeng Zhang,Mingliang Sun,Kailiang Zhang,Baoping Zhang

Proceedings of the National Academy of Sciences of : PubMed31843915

2019

Photoreceptor disc membranes are formed through an Arp2/3-dependent lamellipodium-like mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

William J Spencer,Tylor R Lewis,Sebastien Phan,Martha A Cady,Ekaterina O Serebrovskaya,Nicholas F Schneider,Keun-Young Kim,Lisa A Cameron,Nikolai P Skiba,Mark H Ellisman,Vadim Y Arshavsky

The Journal of cell biology 194:889-904 PubMed21930776

2011

Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology.

Applications

Species

Mouse

Giordano Lippi,Joern R Steinert,Emma L Marczylo,Sabina D'Oro,Roberto Fiore,Ian D Forsythe,Gerhard Schratt,Michele Zoli,Pierluigi Nicotera,Kenneth W Young

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p21-ARC/ARPC3 antibody

Western blot - Anti-p21-ARC/ARPC3 antibody (AB37916)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise