Goat Polyclonal p21-ARC/ARPC3 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications.
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Publications
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- Proceedings of the National Academy of Sciences of :2019Photoreceptor disc membranes are formed through an Arp2/3-dependent lamellipodium-like mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam J Spencer et. al.PubMed 31843915
- The Journal of cell biology 194:889-9042011Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology.Applications:WBReactive species:MouseGiordano Lippi,Joern R Steinert,Emma L Marczylo,Sabina D'Oro,Roberto Fiore,Ian D Forsythe,Gerhard Schratt,Michele Zoli,Pierluigi Nicotera,Kenneth W YoungPubMed 21930776
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
| Rat | Predicted |
| Cow | Predicted |
| Xenopus laevis | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
Target data
Function
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Alternative names
ARC21, ARPC3, Actin-related protein 2/3 complex subunit 3, Arp2/3 complex 21 kDa subunit, p21-ARC
Recommended products
Goat Polyclonal p21-ARC/ARPC3 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p21-ARC also known as ARPC3 is a subunit of the actin-related protein 2/3 complex (Arp2/3 complex). This protein plays a critical role in the nucleation of new actin filaments promoting the formation of branched actin networks. ARPC3 has a molecular mass of approximately 21 kDa and its expression can be found in a variety of tissues including the heart and kidney. The protein operates in the cytoplasm where it associates with other proteins in the Arp2/3 complex to mediate its function.
- Biological function summary
The p21-ARC/ARPC3 is an essential component of the highly conserved Arp2/3 complex involved in actin filament assembly and organization. This complex binds to the sides of pre-existing actin filaments and nucleates new ones creating branching and forming web-like structures needed for various cellular processes. The Arp2/3 complex including ARPC3 is involved in cell movement endocytosis and intracellular trafficking. This protein's activity enhances the dynamic restructuring of the actin cytoskeleton necessary for these functions.
- Pathways
ARPC3 influences pathways related to cell motility and cytoskeletal reorganization. It participates in the Wiskott-Aldrich Syndrome Protein (WASP) pathway where it acts alongside proteins such as WASP and the WAVE complex to regulate actin polymerization. The process converts signals from the cell surface into mechanical force driving the extension of pseudopodia and lamellipodia. Another important pathway involves the Rho family GTPases where ARPC3 links to actin dynamics cell polarity and the orchestration of actin assembly in response to signaling events within the cell.
- Associated diseases and disorders
Abnormalities in ARPC3 function or expression can influence neurological and immunological conditions. For instance dysregulation of the Arp2/3 complex can enhance susceptibility to diseases like Wiskott-Aldrich syndrome which is tied to impaired signaling and cytoskeletal rearrangement. ARPC3 interacts with proteins related to immune response such as WASP making it significant in the context of immune deficiency disorders. Furthermore mutations affecting actin cytoskeleton dynamics can lead to neurodegenerative diseases highlighting the important impact of ARPC3 and associated Arp2/3 complex proteins in maintaining cellular function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-p21-ARC/ARPC3 antibody (ab37916)
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
All lanes: Western blot - Anti-p21-ARC/ARPC3 antibody (ab37916) at 1 µg/mL
All lanes: Mouse Brain lysate (RIPA buffer 35µg total protein per lane).
Predicted band size: 21 kDa
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