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AB247573

Anti-p23 antibody [EPR3846] - BSA and Azide free

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Rabbit Recombinant Monoclonal p23 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

P23, TEBP, PTGES3, Prostaglandin E synthase 3, Cytosolic prostaglandin E2 synthase, Hsp90 co-chaperone, Progesterone receptor complex p23, Telomerase-binding protein p23, cPGES

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-p23 antibody [EPR3846] - BSA and Azide free (AB247573)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p23 antibody [EPR3846] - BSA and Azide free (AB247573)

This data was developed using ab92503, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells, using ab92503 at a dilution of 1/100.

Western blot - Anti-p23 antibody [EPR3846] - BSA and Azide free (AB247573)
  • WB

Lab

Western blot - Anti-p23 antibody [EPR3846] - BSA and Azide free (AB247573)

This data was developed using ab92503, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab92503 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

ab92503 Anti-p23 antibody [EPR3846] was shown to specifically react with p23 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266791 (knockout cell lysate ab258151) was used. Wild-type and p23 knockout samples were subjected to SDS-PAGE. ab92503 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-p23 antibody [EPR3846] (<a href='/en-us/products/primary-antibodies/p23-antibody-epr3846-ab92503'>ab92503</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

PTGES3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PTGES3 (p23) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ptges3-p23-knockout-hek-293t-cell-line-ab266791'>ab266791</a>)

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 19 kDa

Observed band size: 23 kDa

false

Western blot - Anti-p23 antibody [EPR3846] - BSA and Azide free (AB247573)
  • WB

Unknown

Western blot - Anti-p23 antibody [EPR3846] - BSA and Azide free (AB247573)

This data was developed using ab92503, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-p23 antibody [EPR3846] (<a href='/en-us/products/primary-antibodies/p23-antibody-epr3846-ab92503'>ab92503</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human spleen lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

Observed band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3846

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab247573 is the carrier-free version of ab92503.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p23 protein also known as prostaglandin E synthase 3 weighs about 18 kDa. It functions as a co-chaperone for the heat shock protein 90 (Hsp90) complex. P23 is found in the cytoplasm and nucleus of cells and shows expression across various tissues including the brain liver and kidney. It plays a role in stabilizing the ATP-bound state of Hsp90 assisting in client protein stabilization and maturation. P23 binding to the Hsp90 complex influences its chaperone activity.
Biological function summary

P23 assists Hsp90 in protein-folding processes. As part of the Hsp90 chaperone complex p23 contributes to the correct folding and functioning of several essential client proteins. This chaperone activity is important for cellular processes like signal transduction and protein degradation. Without p23 Hsp90's efficiency in maintaining protein homeostasis can decrease highlighting the importance of their interaction in the cell.

Pathways

The chaperone actions of p23 extensively impact protein signaling pathways and stress response mechanisms. P23 and Hsp90 are important in the Akt/PI3K signaling pathway which regulates cell proliferation and survival. The complex formed by p23 and Hsp90 also involves heat shock proteins like Hsp70 which collaborates in broader cellular stress responses. These connections demonstrate how p23 integrates into cellular regulation and signal transduction networks.

P23's interaction with Hsp90 relates to cancer development and progression. Dysregulation in the p23-Hsp90 interaction can lead to increased stabilization of oncogenic client proteins contributing to tumorigenesis. Additionally p23 implication has been noted in neurodegenerative diseases like Alzheimer's where protein misfolding plays a critical role. Here p23 involvement with proteins like Tau may link its chaperone activity to the pathological protein aggregation seen in such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) (PubMed : 10922363). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes (PubMed : 11274138, PubMed : 12077419). Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway (PubMed : 24711448).
See full target information PTGES3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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