AB137736

Anti-p27 KIP 1 antibody

Be the first to review this product! Submit a review

|

(9 Publications)

Rabbit Polyclonal p27 KIP 1 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDKN1B aa 50 to C-terminus.

View Alternative Names

KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody (AB137736)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody (AB137736)

Immunohistochemical analysis of paraffin embedded human ulcerative colitis tissue labelling p27 KIP 1 with ab137736 antibody.

Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody (AB137736)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody (AB137736)

Immunofluorescence staining of paraformaldehyde-fixed (4% paraformaldehyde, 15 minutes at RT) HeLa cells stained for p27 KIP1 with ab137736 at 1 : 500 dilution. Red : phalloidin, a cytoskeleton marker, diluted at 1 : 200.

Immunohistochemistry (Frozen sections) - Anti-p27 KIP 1 antibody (AB137736)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-p27 KIP 1 antibody (AB137736)

Frozen sections of adult mouse retina tissue stained for p27 KIP1 using ab137736 at 1 : 250 dilution in immunohistochemical analysis. Red : protein kinase C alpha staining. Blue : Fluoroshield with DAPI.

Western blot - Anti-p27 KIP 1 antibody (AB137736)

WB

Supplier Data

Western blot - Anti-p27 KIP 1 antibody (AB137736)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-p27 KIP 1 antibody (ab137736) at 1/500 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

p27 Kip1 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, IHC-Fr, IHC-P, ICC/IF

Immunogen

Recombinant Fragment Protein within Human CDKN1B aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Alternative Products

Primary Antibodies

AB32034

Anti-p27 KIP 1 antibody [Y236]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [Y236] (AB32034)

4
18

Primary Antibodies

AB92741

Anti-p27 KIP 1 antibody [EPFHCR16]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (AB92741)

1
1

Primary Antibodies

AB190851

Anti-p27 KIP 1 antibody [EPR18388-138]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] (AB190851)

0
0

Primary Antibodies

AB113075

Anti-p27 KIP 1 antibody [EPFHCR16a]

RabMAb|Recombinant|KO Validated

Western blot - Anti-p27 KIP 1 antibody [EPFHCR16a] (AB113075)

0
0

Primary Antibodies

AB206927

Anti-p27 KIP 1 antibody [Y236] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-p27 KIP 1 antibody [Y236] - BSA and Azide free (AB206927)

4
2

Complementary Products

Primary Antibodies

AB109520

Anti-p21 antibody [EPR362]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-p21 antibody [EPR362] (AB109520)

4
16

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

5
24

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

5
133

Primary Antibodies

AB134175

Anti-Cyclin D1 antibody [EPR2241] - C-terminal

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Cyclin D1 antibody [EPR2241] - C-terminal (AB134175)

5
15

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.

Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed : 28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

See full target information CDKN1B

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Computational and mathematical methods in medicine 2022:1450098 PubMed36262872

2022

miR-302a-3p Promotes Radiotherapy Sensitivity of Hepatocellular Carcinoma by Regulating Cell Cycle via MCL1.

Applications

Unspecified application

Species

Unspecified reactive species

Zifeng Yang,Menglong Zhang,Jian Zhang,Cunkun Chu,Bijuan Hu,Liyin Huang

Cytogenetic and genome research 161:488-500 PubMed34923485

2021

Silencing of miR-1246 Induces Cell Cycle Arrest and Apoptosis in Cisplatin-Resistant Ovarian Cancer Cells by Promoting ZNF23 Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Cai,Qian Zhang,Lili Du,Feiyun Zheng

Archives of biochemistry and biophysics 688:108404 PubMed32416101

2020

IL-10 regulates the malignancy of hemangioma-derived endothelial cells via regulation of PCNA.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaofan Zeng,Hao Chen,Junhong Cai,Yanjing Huang,Jie Yue

Cellular and molecular life sciences : CMLS 77:903-918 PubMed31312877

2019

miR-221 and -222 target CACNA1C and KCNJ5 leading to altered cardiac ion channel expression and current density.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Binas,Maria Knyrim,Julia Hupfeld,Udo Kloeckner,Sindy Rabe,Sigrid Mildenberger,Katja Quarch,Nicole Strätz,Danny Misiak,Michael Gekle,Claudia Grossmann,Barbara Schreier

Journal of cellular physiology 235:176-184 PubMed31210352

2019

MicroRNA-132 protects H9c2 cells against oxygen and glucose deprivation-evoked injury by targeting FOXO3A.

Applications

Unspecified application

Species

Unspecified reactive species

Jingze Zhang,Haiming Xu,Licheng Gong,Long Liu

Scientific reports 9:8028 PubMed31142771

2019

Interplay of fibroblasts with anaplastic tumor cells promotes follicular thyroid cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Fozzatti,Vanina Alejandra Alamino,Sunmi Park,Lucila Giusiano,Ximena Volpini,Li Zhao,Cinthia Carolina Stempin,Ana Carolina Donadio,Sheue-Yann Cheng,Claudia Gabriela Pellizas

Molecular medicine reports 18:5552-5562 PubMed30387828

2018

UBA2 promotes proliferation of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ping He,Xun Sun,Hong-Jing Cheng,Ya-Bin Zou,Quan Wang,Chang-Li Zhou,Wan-Qi Liu,Yue-Ming Hao,Xiang-Wei Meng

Molecular medicine reports 14:3917-24 PubMed27572672

2016

Inhibiting the role of Skp2 suppresses cell proliferation and tumorigenesis of human gastric cancer cells via the upregulation of p27kip1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanguang Wen,Kuansong Wang,Kaiyan Yang

Oncology reports 33:133-40 PubMed25369910

2014

Ubiquitin specific protease 22 promotes cell proliferation and tumor growth of epithelial ovarian cancer through synergy with transforming growth factor β1.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Ji,Huirong Shi,Ya Xie,Zhao Zhao,Shunshuang Li,Cheng Chang,Xinghan Cheng,Yue Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com