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AB3928

Anti-p27 KIP 1 antibody [DCS-72.F6]

4

(2 Reviews)

|

(3 Publications)

Mouse Monoclonal p27 KIP 1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Cdkn1b.

View Alternative Names

Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [DCS-72.F6] (AB3928)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [DCS-72.F6] (AB3928)

ab3928 staining human colon carcinoma by IHC-P.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DCS-72.F6

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Cdkn1b.

P46414

Epitope

Between amino acids 83-204 of p27.

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Alternative Products

Primary Antibodies

AB92741

Anti-p27 KIP 1 antibody [EPFHCR16]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (AB92741)

  • 1
  • 1
Primary Antibodies

AB190851

Anti-p27 KIP 1 antibody [EPR18388-138]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] (AB190851)

  • 0
  • 0
Primary Antibodies

AB32034

Anti-p27 KIP 1 antibody [Y236]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [Y236] (AB32034)

  • 4
  • 18
Primary Antibodies

AB113075

Anti-p27 KIP 1 antibody [EPFHCR16a]

RabMAb|Recombinant|KO Validated

Western blot - Anti-p27 KIP 1 antibody [EPFHCR16a] (AB113075)

  • 0
  • 0
Primary Antibodies

AB206927

Anti-p27 KIP 1 antibody [Y236] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-p27 KIP 1 antibody [Y236] - BSA and Azide free (AB206927)

  • 4
  • 2

Complementary Products

Assay Kits

AB313917

Human p27 Kip 1 ELISA Kit

Sandwich ELISA - Human p27 Kip 1 ELISA Kit (AB313917)

  • 0
  • 0
Primary Antibodies

AB109520

Anti-p21 antibody [EPR362]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-p21 antibody [EPR362] (AB109520)

  • 4
  • 16
Primary Antibodies

AB16663

Anti-Cyclin D1 antibody [SP4]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials

Western blot - Anti-Cyclin D1 antibody [SP4] (AB16663)

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  • 23
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32147

Anti-Cdk2 antibody [E304]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Cdk2 antibody [E304] (AB32147)

  • 5
  • 8
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important regulator of cell cycle progression (PubMed : 12972555, PubMed : 8033213). Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (By similarity). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes (PubMed : 8033213). Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
See full target information Cdkn1b
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0152025 PubMed27011052

2016

Notch Signaling Activates Stem Cell Properties of Müller Glia through Transcriptional Regulation and Skp2-mediated Degradation of p27Kip1.

Applications

IHC-Fr, ICC/IF, WB

Species

Rat, Rat, Rat

Carolina Beltrame Del Debbio,Qulsum Mir,Sowmya Parameswaran,Saumi Mathews,Xiaohuan Xia,Li Zheng,Andrew J Neville,Iqbal Ahmad

Nature genetics 46:516-21 PubMed24658003

2014

TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function.

Applications

IF

Species

Human

Fernando Gómez-Herreros,Janneke H M Schuurs-Hoeijmakers,Mark McCormack,Marie T Greally,Stuart Rulten,Rocío Romero-Granados,Timothy J Counihan,Elijah Chaila,Judith Conroy,Sean Ennis,Norman Delanty,Felipe Cortés-Ledesma,Arjan P M de Brouwer,Gianpiero L Cavalleri,Sherif F El-Khamisy,Bert B A de Vries,Keith W Caldecott

BMC infectious diseases 9:125 PubMed19664251

2009

Modulations of cell cycle checkpoints during HCV associated disease.

Applications

Unspecified application

Species

Unspecified reactive species

Saira Sarfraz,Saeed Hamid,Syed Ali,Wasim Jafri,Anwar A Siddiqui
View all publications
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Product promise

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