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Rabbit Recombinant Monoclonal p27 KIP 1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Cited in 15 publications.


Images

Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (AB92741), expandable thumbnail
  • Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (AB92741), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (AB92741), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (AB92741), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (AB92741), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% EDTA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Mouse
Tested
Not recommended
Not recommended
Not recommended
Tested
Rat
Tested
Not recommended
Not recommended
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Important regulator of cell cycle progression (PubMed:12972555, PubMed:8033213). Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (By similarity). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes (PubMed:8033213). Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Alternative names

Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b

Recommended products

Rabbit Recombinant Monoclonal p27 KIP 1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Cited in 15 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPFHCR16
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Species reactivity
Human: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.

Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Associated diseases and disorders

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741), expandable thumbnail

    Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

    False colour image of Western blot: Anti-p27 KIP 1 antibody [EPFHCR16] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92741 was shown to bind specifically to p27 KIP 1. A band was observed at 28 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in CDKN1B knockout cell line Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell line ab281619 (knockout cell lysate Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell lysate ab282970). To generate this image, wild-type and CDKN1B knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741) at 1/1000 dilution

    Lane 1: Wild-type RAW 264.7 cell lysate at 20 µg

    Lane 2: CDKN1B knockout RAW 264.7 cell lysate at 20 µg

    Lane 2: Western blot - Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell line (Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell line ab281619)

    Performed under reducing conditions.

    Predicted band size: 22 kDa

    Observed band size: 28 kDa

  • Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741), expandable thumbnail

    Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

    All lanes: Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741) at 1/1000 dilution

    Lane 1: NIH/3T3 cell lysate at 10 µg

    Lane 2: C6 cell lysate at 10 µg

    Lane 3: Neuro-2a cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 22 kDa

    Observed band size: 27 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of mouse testis is observed (PMID: 10098522). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody.

    Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (Universal HIER antigen retrieval reagent (10X) ab208572).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of rat testis is observed (PMID: 10098522). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody.

    Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (Universal HIER antigen retrieval reagent (10X) ab208572).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

    ab92741 at 1/100 dilution staining p27 KIP in Mouse lung by Immunohistochemistry, Paraffin-embedded tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

    ab92741 at 1/100 dilution staining p27 KIP in Mouse thymus by Immunohistochemistry, Paraffin-embedded tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

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Product protocols

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