Rabbit Recombinant Monoclonal p27 KIP 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunoprecipitation-img5034.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911)")
![Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunocytochemistry-immunofluorescence-img72975.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129889.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95016.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117460.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (AB227911)")
Publications
Filter by
- Cancer medicine 9:5976-59882020Exosomes released from M2 macrophages transfer miR-221-3p contributed to EOC progression through targeting CDKN1B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoduan Li et. al.PubMed 32590883
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Mouse | Tested | Expected | Tested | Expected |
| Rat | Expected | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Important regulator of cell cycle progression (PubMed:8033213, PubMed:12972555). Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (By similarity). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes (PubMed:8033213). Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Alternative names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b
Recommended products
Rabbit Recombinant Monoclonal p27 KIP 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR18388-138
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab227911 is the carrier-free version of Anti-p27 KIP 1 antibody [EPR18388-138] ab190851.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
- Biological function summary
The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.
- Pathways
The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.
- Associated diseases and disorders
Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunoprecipitation-img5034.jpg "Immunoprecipitation - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)")
Immunoprecipitation - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)
p27 KIP 1 was immunoprecipitated from 0.35 mg of C2C12 (mouse myoblast cell line) whole cell lysate with Anti-p27 KIP 1 antibody [EPR18388-138] ab190851 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-p27 KIP 1 antibody [EPR18388-138] ab190851 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as for detection at 1/10000 dilution.
Lane 1: C2C12 whole cell lysate 10 μg (Input).
Lane 2: Anti-p27 KIP 1 antibody [EPR18388-138] ab190851 IP in C2C12 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-p27 KIP 1 antibody [EPR18388-138] ab190851 in C2C12 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p27 KIP 1 antibody [EPR18388-138] ab190851).
All lanes: Immunoprecipitation - Anti-p27 KIP 1 antibody [EPR18388-138] (Anti-p27 KIP 1 antibody [EPR18388-138] ab190851)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 27 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunocytochemistry-immunofluorescence-img72975.jpg "Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)")
Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling p27 KIP 1 with Anti-p27 KIP 1 antibody [EPR18388-138] ab190851 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly nuclear staining in the Neuro-2a cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p27 KIP 1 antibody [EPR18388-138] ab190851).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129889.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling p27 KIP 1 with Anti-p27 KIP 1 antibody [EPR18388-138] ab190851 at 1/40000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Nuclear staining on mouse kidney is observed (PMID: 26823281). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p27 KIP 1 antibody [EPR18388-138] ab190851).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95016.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling p27 KIP 1 with Anti-p27 KIP 1 antibody [EPR18388-138] ab190851 at 1/40000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Nuclear staining on Sertoli cells and Leydig cells of rat testis is observed (PMID: 10098522). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p27 KIP 1 antibody [EPR18388-138] ab190851).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117460.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling p27 KIP 1 with Anti-p27 KIP 1 antibody [EPR18388-138] ab190851 at 1/40000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Nuclear staining in the rat cerebral cortex is observed (PMID: 19852587). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p27 KIP 1 antibody [EPR18388-138] ab190851).
![Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunocytochemistry-immunofluorescence-img74238.jpg "Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)")
Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling p27 KIP 1 with Anti-p27 KIP 1 antibody [EPR18388-138] ab190851 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly nuclear staining in the NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p27 KIP 1 antibody [EPR18388-138] ab190851).
![Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-antibody-epr18388-138-bsa-and-azide-free-ab227911--immunocytochemistry-immunofluorescence-img74238.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] - BSA and Azide free (ab227911)")
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