AB212252

Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free

Name: Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free (ab212252) | Abcam
Brand: Abcam Limited
SKU: AB212252
Price: 905 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal p27 KIP 1 antibody. Carrier free. Suitable for IHC-P, Flow Cyt and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CDKN1B.

View Alternative Names

KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free (AB212252)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free (AB212252)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate cancer tissue labeling p27 Kip1 with ab212252 at 0.5 μg/mL.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free (AB212252)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling p27 Kip1 with ab212252 at 0.5 μg/mL.

Flow Cytometry - Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free (AB212252)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free (AB212252)

Flow Cytometry analysis of Jurkat cells labeling p27 Kip1 with ab212252 at 1 μg/mL : Green.
Black : Jurkat cells alone. Grey : Isotype control.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free (AB212252)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cervical cancer tissue labeling p27 Kip1 with ab212252 at 0.5 μg/mL.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [KIP1/769] - BSA and Azide free (AB212252)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colon tissue labeling p27 Kip1 with ab212252 at 0.5 μg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KIP1/769

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat, Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CDKN1B. The exact immunogen used to generate this antibody is proprietary information.

P46527

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-1 µg for 10^6 Cells", "FlowCyt-species-notes": "In 0.1ml." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.

Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed : 28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

See full target information CDKN1B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 12:7015-7024 PubMed31695416

2019

MicroRNA-30a-3p inhibits the progression of lung cancer via the PI3K/AKT by targeting DNA methyltransferase 3a.

Applications

Unspecified application

Species

Unspecified reactive species

Desheng Wei,Guangmao Yu,Yeping Zhao

View all publications

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Product promise

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