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AB193379

Anti-p27 KIP 1 antibody [SX53G8]

5

(3 Reviews)

|

(22 Publications)

Knockout Tested Mouse Monoclonal p27 KIP 1 antibody. Suitable for IHC-P, ICC, IP, Flow Cyt, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat, Monkey samples. Cited in 22 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDKN1B.

View Alternative Names

KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [SX53G8] (AB193379)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [SX53G8] (AB193379)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling p27 KIP 1 with ab193379 at 1 μg/mL.

Immunocytochemistry - Anti-p27 KIP 1 antibody [SX53G8] (AB193379)
  • ICC

Unknown

Immunocytochemistry - Anti-p27 KIP 1 antibody [SX53G8] (AB193379)

Immunocytochemical analysis of PFA-fixed MCF-7 cells labeling p27 KIP 1 with ab193379 at 1 μg/mL. A Goat anti-mouse IgG CF488 antibody (Green) was used as a secondary antibody. The membrane is stained with Phalloidin-CF640

Western blot - Anti-p27 KIP 1 antibody [SX53G8] (AB193379)
  • WB

Lab

Western blot - Anti-p27 KIP 1 antibody [SX53G8] (AB193379)

Western blot : Anti-CDKN1B antibody [SX53G8] (ab193379) staining at 1 ug/ml, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab193379 was shown to bind specifically to CDKN1B. A band was observed at 30 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in CDKN1B knockout cell line. To generate this image, wild-type and CDKN1B knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-p27 KIP 1 antibody [SX53G8] (ab193379) at 1 µg/mL

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

CDKN1B knockout HCT 116 cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

CDKN1B knockout HAP1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Observed band size: 30 kDa

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Western blot - Anti-p27 KIP 1 antibody [SX53G8] (AB193379)
  • WB

Lab

Western blot - Anti-p27 KIP 1 antibody [SX53G8] (AB193379)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : CDKN1B knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : MCF7 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab193379 observed at 30 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab193379 was shown to specifically recognize CDKN1B in wild-type HAP1 cells as well as additional cross-reactive bands. No bands were observed when CDKN1B knockout samples were used. Wild-type and CDKN1B knockout samples were subjected to SDS-PAGE. ab193379 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/mL and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-p27 KIP 1 antibody [SX53G8] (ab193379)

Predicted band size: 22 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SX53G8

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human, Monkey, Mouse, Rat

Applications

IHC-Fr, Flow Cyt, WB, ICC/IF, ICC, IP, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CDKN1B.

P46527

Specificity

ab193379 is highly specific and shows no cross-reaction with other related mitotic inhibitors.

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Reactivity data

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Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed : 28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
See full target information CDKN1B
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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Cytotechnology 76:217-229 PubMed38495297

2024

Therapeutic potential of exosome derived from hepatocyte growth factor-overexpressing adipose mesenchymal stem cells in TGFβ1-stimulated hepatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wang,Weikang Ye,Ming Jiang,Yinong Zhou,Jie Zheng

Life science alliance 6: PubMed37197981

2023

Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Bipul R Acharya,Jennifer S Fang,Erin D Jeffery,Nicholas W Chavkin,Gael Genet,Hema Vasavada,Elizabeth A Nelson,Gloria M Sheynkman,Martin J Humphries,Karen K Hirschi

Molecular therapy : the journal of the American Society of Gene Therapy 31:847-865 PubMed36639869

2023

ABRO1 arrests cardiomyocyte proliferation and myocardial repair by suppressing PSPH.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Lu-Yu Zhou,Xin-Min Li,Fang Liu,Lin Liang,Xin-Zhe Chen,Jie Ju,Murugavel Ponnusamy,Kai Wang,Cui-Yun Liu,Kao-Wen Yan,Kun Wang

Technology in cancer research & treatment 21:15330338221119745 PubMed35971329

2022

Treatment with b-AP15 to Inhibit UCHL5 and USP14 Deubiquitinating Activity and Enhance p27 and Cyclin E1 for Tumors with Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Zong-Yuan Jiang,Jiang Hong,Ju-Hua Zhang,Xiao-Feng Wang,Yu-Shui Ma,Zhang-Xia Xiong,Hao-Ran Sun,Cong Cheng,Bang-Zhu Xie,Ji-Bin Liu,Yang-Gang Ouyang,Da Fu

Cell death & disease 13:314 PubMed35393397

2022

A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/Prc1 (and Nuf2) axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Jianfei Chen,Bohan Li,Qingling Jiang,Ling Liu,Ziyi Xia,Qiusheng Zheng,Minjing Li,Defang Li

Molecular therapy. Nucleic acids 27:122-132 PubMed34976432

2022

rAAV-delivered PTEN therapeutics for prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhong Ai,Jia Li,Qin Su,Hong Ma,Qiang Wei,Hong Li,Guangping Gao

Cell death & disease 12:1039 PubMed34725331

2021

Paradoxical implication of BAX/BAK in the persistence of tetraploid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayin Deng,Lucía G Gutiérrez,Gautier Stoll,Omar Motiño,Isabelle Martins,Lucía Núñez,José Manuel Bravo-San Pedro,Juliette Humeau,Chloé Bordenave,Juncheng Pan,Hélène Fohrer-Ting,Sylvie Souquere,Gerard Pierron,Claudio Hetz,Carlos Villalobos,Guido Kroemer,Laura Senovilla

Journal of cellular and molecular medicine 25:8890-8903 PubMed34382342

2021

Knockdown of CENPK inhibits cell growth and facilitates apoptosis via PTEN-PI3K-AKT signalling pathway in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shusheng Wu,Lulu Cao,Lihong Ke,Ying Yan,Huiqin Luo,Xiaoxiu Hu,Jiayu Niu,Huimin Li,Huijun Xu,Wenju Chen,Yueyin Pan,Yifu He

The journal of international advanced otology 17:306-312 PubMed34309550

2021

Differential Expression of Ki-67 and P27 in Cholesteatoma Compared to Skin Tissue Predicts the Prognosis of Adult Acquired Cholesteatoma.

Applications

Unspecified application

Species

Unspecified reactive species

Serkan Türkili,Kemal Görür,Onur İsmi,Ebru Serinsöz Linke,Yusuf Vayısoğlu,Cengiz Özcan

Cell death & disease 12:275 PubMed33723215

2021

FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Qinkai Zhang,Xunzhu Zhou,Maoping Wan,Xixi Zeng,Jiarong Luo,Yesha Xu,Liying Ji,Jian-An Zhang,Pei Fan,Jianing Zhong,Jianmin Wu
View all publications
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