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AB62364

Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y]

5

(6 Reviews)

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(45 Publications)

Rabbit Recombinant Monoclonal p27 KIP 1 phospho S10 antibody. Suitable for WB, IP, Dot, IHC-P and reacts with Mouse, Rat, Human, Monkey, Synthetic peptide samples. Cited in 45 publications.

View Alternative Names

KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling p27 KIP 1 with Purified ab62364 at 1 : 100 dilution (4.19 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)
  • IP

Unknown

Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)

Purified ab62364 at 1/50 dilution (2μg) immunoprecipitating p27 KIP 1 in MCF7 whole cell lysate.
Lane 1 (input) : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab62364 + MCF7 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab62364 in MCF7 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 27 kDa

All lanes:

Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)

Predicted band size: 22 kDa

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Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)
  • WB

Unknown

Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Lane 6:

C6 (Rat glial tumor glial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 27 kDa

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Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)
  • Dot

Unknown

Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)

Dot blot analysis of p27 KIP 1 (pS10) phospho peptide (Lane 1) and p27 KIP 1 non-phospho peptide (Lane 2) using ab62364 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

Blocking and Diluting buffer and concentration : 5% NFDM /TBST.

Exposure time : 3 minutes.

Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)
  • WB

CiteAb

Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)

p27 KIP 1 (phospho S10) western blot using anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364. Publication image and figure legend from van der Laden, J., Soppa, U., et al., 2015, Cell Commun Signal, PubMed 25630557.

ab62364 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab62364 please see the product overview.

Maximal activity of HIPKs depends on the activation loop tyrosine. Wild type GFP-HIPK fusion proteins and the respective Tyr→Phe mutants were immunoprecipitated from HeLa cells and subjected to kinase assays with recombinant GST-p27Kip1(A), myelin basic protein (B) or DYRKtide (C). GFP served as background control. A, Phosphorylation of p27Kip1 at Ser10 was detected by immunoblot with a phosphorylation-specific antibody. For quantitative evaluation, pSer10 immunoreactivity was normalised to GFP immunoreactivity, which reflects the amount of kinase in the reaction. The blots illustrate a representative experiment, and the relative catalytic activities as determined from 3–4 assays are shown below the panels (means ± SD). One-sample t test : *, p < 0.05; **, p < 0.01. B and C, Phosphorylation of MBP and DYRKtide was measured in triplicate as incorporation of 32P. Background values from the GFP control samples were subtracted and activities were normalised to the amount of kinase in the reaction as determined by GFP immunoreactivity. Column diagrams illustrate catalytic activities relative to HIPK2 (WT). The results were replicated in independent experiments, except for a missing value of HIPK1.

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  • Carrier free

    Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP233(2)Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Monkey

Applications

Dot, WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects p27 KIP 1 phosphorylated at Serine 10.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p><strong>For unpurified use at 1/200 - 1/1500</strong></p><p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p><strong>For unpurified use at 1/200 - 1/1500</strong></p><p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed : 28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
See full target information CDKN1B phospho S10
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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:15 PubMed39828766

2025

Isoxazole-based molecules restore NK cell immune surveillance in hepatocarcinogenesis by targeting TM4SF5 and SLAMF7 linkage.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Eon Kim,Hyun Su Kim,Wonsik Kim,Eun Hae Lee,Soyeon Kim,Taewoo Kim,Eun-Ae Shin,Kyung-Hee Pyo,Haesong Lee,Seo Hee Jin,Jae-Ho Lee,Soo-Min Byeon,Dong Joo Kim,Jinwook Jeong,Jeongwon Lee,Minjae Ohn,Hyojung Lee,Su Jong Yu,Dongyun Shin,Semi Kim,Jun Yeob Yoo,Seung-Chul Lee,Young-Ger Suh,Jung Weon Lee

Histology and histopathology 40:73-87 PubMed38818655

2024

p27 and cytoplasmic pSer10p27 are promising biomarkers for predicting prognosis and chemotherapy response in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mengna Zhu,Si Sun,Lin Huang,Lingling Gao,Mengqing Chen,Jing Cai,Zehua Wang,Minggang Peng

Theranostics 14:1371-1389 PubMed38389850

2024

Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Gao,Tuo Zhang,Tengxiang Chen,Ziqi Chen,Zijian Zhu,Yang Wen,Shaogang Qin,Yibing Bao,Ting Zhao,Hengxing Li,Longping Liu,Ming Hao,Jianbin Wang,Fengchao Wang,Haibin Wang,Bo Zhou,Hua Zhang,Guoliang Xia,Chao Wang

International journal of molecular sciences 24: PubMed38138967

2023

Dual-Specificity Phosphatase 6 Deficiency Attenuates Arterial-Injury-Induced Intimal Hyperplasia in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Candra D Hamdin,Meng-Ling Wu,Chen-Mei Chen,Yen-Chun Ho,Wei-Cheng Jiang,Pei-Yu Gung,Hua-Hui Ho,Huai-Chia Chuang,Tse-Hua Tan,Shaw-Fang Yet

Experimental & molecular medicine 54:2022-2035 PubMed36424455

2022

Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenjun Yu,Yuhan Li,Shuai Shao,Beichen Guo,Mengxia Zhang,Lina Zheng,Kun Zhang,Feng Zhou,Li Zhang,Chiyi Chen,Wentao Jiang,Wei Hong,Tao Han

Regenerative therapy 21:560-573 PubMed36475023

2022

Proteomics reveals the potential mechanism of Tanshinone IIA in promoting the expansion of human bone marrow mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Yuan,Hong-Yan Qin,Jia-Yun Wei,Geshuyi Chen,Xun Li

Journal of cellular and molecular medicine 26:2981-2994 PubMed35429093

2022

LRP5 regulates cardiomyocyte proliferation and neonatal heart regeneration by the AKT/P21 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huixing Zhou,Fulei Zhang,Yahan Wu,Hongyu Liu,Ran Duan,Yuanyuan Liu,Yan Wang,Xiaoyu He,Yuemei Zhang,Xiue Ma,Yi Guan,Yi Liu,Dandan Liang,Liping Zhou,Yi-Han Chen

Computational and mathematical methods in medicine 2022:2329576 PubMed35082911

2022

miR-335-5p Inhibits Progression of Uterine Leiomyoma by Targeting ARGLU1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhao,Yingyan Zhao,Ling Chen,Yan Sun,Sumei Fan

BioFactors (Oxford, England) 48:135-147 PubMed34856026

2021

Lnc-hipk1 inhibits mouse adipocyte apoptosis as a sponge of miR-497.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Wu,Hong Yang,Ruiqing Tai,Chaowei Li,Tianyu Xia,Yongnian Liu,Chao Sun

Nature communications 12:4671 PubMed34344863

2021

Simultaneous CK2/TNIK/DYRK1 inhibition by 108600 suppresses triple negative breast cancer stem cells and chemotherapy-resistant disease.

Applications

Unspecified application

Species

Unspecified reactive species

Katsutoshi Sato,Amol A Padgaonkar,Stacey J Baker,Stephen C Cosenza,Olga Rechkoblit,D R C Venkata Subbaiah,Josep Domingo-Domenech,Alison Bartkowski,Elisa R Port,Aneel K Aggarwal,M V Ramana Reddy,Hanna Y Irie,E Premkumar Reddy
View all publications
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Product promise

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