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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Recombinant Monoclonal p27 KIP 1 phospho S10 antibody. Suitable for IP, WB, Dot, IHC-P and reacts with Human, Mouse, Rat, Monkey, Synthetic peptide samples. Cited in 41 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | IP | WB | Flow Cyt | Dot | IHC-P | |
---|---|---|---|---|---|---|
Human | Not recommended | Tested | Tested | Not recommended | Expected | Tested |
Mouse | Not recommended | Expected | Predicted | Not recommended | Predicted | Expected |
Rat | Not recommended | Expected | Predicted | Not recommended | Predicted | Predicted |
Monkey | Not recommended | Expected | Predicted | Not recommended | Predicted | Predicted |
Synthetic peptide | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details. |
Species Rat | Dilution info - | Notes Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details. |
Species Monkey | Dilution info - | Notes Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details. |
Species Human | Dilution info - | Notes Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details. |
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Species Rat | Dilution info 1/50 | Notes - |
Species Monkey | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/20000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Monkey, Human, Mouse, Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes For unpurified use at 1/200 - 1/1500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 | Notes For unpurified use at 1/200 - 1/1500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, KIP1, CDKN1B, p27
Rabbit Recombinant Monoclonal p27 KIP 1 phospho S10 antibody. Suitable for IP, WB, Dot, IHC-P and reacts with Human, Mouse, Rat, Monkey, Synthetic peptide samples. Cited in 41 publications.
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, KIP1, CDKN1B, p27
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EP233(2)Y
Affinity purification Protein A
This antibody detects p27 KIP 1 phosphorylated at Serine 10.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.
The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.
Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Purified ab62364 at 1/50 dilution (2μg) immunoprecipitating p27 KIP 1 in MCF7 whole cell lysate.
Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab62364 + MCF7 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab62364 in MCF7 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 27 kDa
All lanes: Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)
Predicted band size: 22 kDa
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 6: C6 (Rat glial tumor glial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 27 kDa
This data was developed using ab62364, the same antibody clone in a different buffer formulation.
Blocking buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling p27 KIP 1 with Purified ab62364 at 1:100 dilution (4.19 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Dot blot analysis of p27 KIP 1 (pS10) phospho peptide (Lane 1) and p27 KIP 1 non-phospho peptide (Lane 2) using ab62364 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: 3 minutes.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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