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Rabbit Recombinant Monoclonal p27 KIP 1 phospho S10 antibody. Suitable for IP, WB, Dot, IHC-P and reacts with Human, Mouse, Rat, Monkey, Synthetic peptide samples. Cited in 41 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow CytDotIHC-P
Human
Not recommended
Tested
Tested
Not recommended
Expected
Tested
Mouse
Not recommended
Expected
Predicted
Not recommended
Predicted
Expected
Rat
Not recommended
Expected
Predicted
Not recommended
Predicted
Predicted
Monkey
Not recommended
Expected
Predicted
Not recommended
Predicted
Predicted
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.

Species

Rat

Dilution info

-

Notes

Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.

Species

Monkey

Dilution info

-

Notes

Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.

Species

Human

Dilution info

-

Notes

Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Species

Monkey

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500 - 1/20000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Monkey, Human, Mouse, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

For unpurified use at 1/200 - 1/1500

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

1/100

Notes

For unpurified use at 1/200 - 1/1500

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat, Monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Target data

Function

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Alternative names

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Rabbit Recombinant Monoclonal p27 KIP 1 phospho S10 antibody. Suitable for IP, WB, Dot, IHC-P and reacts with Human, Mouse, Rat, Monkey, Synthetic peptide samples. Cited in 41 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP233(2)Y

Purification technique

Affinity purification Protein A

Specificity

This antibody detects p27 KIP 1 phosphorylated at Serine 10.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Activity summary

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Associated diseases and disorders

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364), expandable thumbnail

    Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)

    Purified ab62364 at 1/50 dilution (2μg) immunoprecipitating p27 KIP 1 in MCF7 whole cell lysate.
    Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab62364 + MCF7 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab62364 in MCF7 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 27 kDa

    All lanes: Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364)

    Predicted band size: 22 kDa

  • Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364), expandable thumbnail

    Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)

    Blocking buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (AB62364) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

    Lane 6: C6 (Rat glial tumor glial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 22 kDa

    Observed band size: 27 kDa

    This data was developed using ab62364, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling p27 KIP 1 with Purified ab62364 at 1:100 dilution (4.19 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364), expandable thumbnail

    Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)

    Dot blot analysis of p27 KIP 1 (pS10) phospho peptide (Lane 1) and p27 KIP 1 non-phospho peptide (Lane 2) using ab62364 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

    Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

    Exposure time: 3 minutes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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