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Rabbit Recombinant Monoclonal p27 KIP 1 phospho S10 antibody. Carrier free. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Mouse, Rat, Monkey, Synthetic peptide samples.

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Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytDotWBIHC-P
Human
Not recommended
Tested
Not recommended
Expected
Tested
Tested
Mouse
Not recommended
Expected
Not recommended
Expected
Tested
Expected
Rat
Not recommended
Expected
Not recommended
Expected
Tested
Expected
Monkey
Not recommended
Expected
Not recommended
Predicted
Predicted
Predicted
Synthetic peptide
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Images

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.

Species
Rat
Dilution info
-
Notes

Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.

Species
Human
Dilution info
-
Notes

Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.

Species
Monkey
Dilution info
-
Notes

Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details.

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Monkey, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Monkey
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

For unpurified use at 1/200 - 1/1500

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
-
Notes

-

Species
Mouse
Dilution info
-
Notes

For unpurified use at 1/200 - 1/1500

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Monkey
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Recommended products

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Rabbit Recombinant Monoclonal p27 KIP 1 phospho S10 antibody. Carrier free. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Mouse, Rat, Monkey, Synthetic peptide samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

EP233(2)Y

Purification technique

Affinity purification Protein A

Specificity

This antibody detects p27 KIP 1 phosphorylated at Serine 10.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab228877 is the carrier-free version of ab62364.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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