Rabbit Recombinant Monoclonal p27 KIP 1 phospho S10 antibody. Carrier free. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Mouse, Rat, Monkey, Synthetic peptide samples.
Images
![Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (AB228877), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-phospho-s10-antibody-ep2332y-bsa-and-azide-free-ab228877--immunoprecipitation-img4096.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (AB228877)")
![Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (AB228877), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-phospho-s10-antibody-ep2332y-bsa-and-azide-free-ab228877--western-blot-img30176.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (AB228877)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (AB228877), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-phospho-s10-antibody-ep2332y-bsa-and-azide-free-ab228877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130034.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (AB228877)")
![Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (AB228877), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-phospho-s10-antibody-ep2332y-bsa-and-azide-free-ab228877--dot-blot-img6031.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (AB228877)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | Dot | WB | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Expected | Tested | Tested |
| Mouse | Not recommended | Expected | Not recommended | Expected | Tested | Expected |
| Rat | Not recommended | Expected | Not recommended | Expected | Tested | Expected |
| Monkey | Not recommended | Expected | Not recommended | Predicted | Predicted | Predicted |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details. |
Species Rat | Dilution info - | Notes Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details. |
Species Human | Dilution info - | Notes Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details. |
Species Monkey | Dilution info - | Notes Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for further details. |
Species Synthetic peptide | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Monkey, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For unpurified use at 1/200 - 1/1500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes For unpurified use at 1/200 - 1/1500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Alternative names
KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1
Recommended products
Rabbit Recombinant Monoclonal p27 KIP 1 phospho S10 antibody. Carrier free. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Mouse, Rat, Monkey, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP233(2)Y
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody detects p27 KIP 1 phosphorylated at Serine 10.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab228877 is the carrier-free version of Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
- Biological function summary
The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.
- Pathways
The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.
- Associated diseases and disorders
Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-phospho-s10-antibody-ep2332y-bsa-and-azide-free-ab228877--immunoprecipitation-img4096.jpg "Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)")
Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
This data was developed using Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364, the same antibody clone in a different buffer formulation.
Purified Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364 at 1/50 dilution (2μg) immunoprecipitating p27 KIP 1 in MCF7 whole cell lysate.
Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364 + MCF7 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364 in MCF7 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 27 kDaAll lanes: Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364)
Predicted band size: 22 kDa
![Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-phospho-s10-antibody-ep2332y-bsa-and-azide-free-ab228877--western-blot-img30176.jpg "Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)")
Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
This data was developed using Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364, the same antibody clone in a different buffer formulation.
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 6: C6 (Rat glial tumor glial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-phospho-s10-antibody-ep2332y-bsa-and-azide-free-ab228877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130034.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
This data was developed using Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364 the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling p27 KIP 1 with Purified Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364 at 1:100 dilution (4.19 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.![Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877), expandable thumbnail](https://content.abcam.com/products/images/p27-kip-1-phospho-s10-antibody-ep2332y-bsa-and-azide-free-ab228877--dot-blot-img6031.jpg "Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)")
Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
Dot blot analysis of p27 KIP 1 (pS10) phospho peptide (Lane 1) and p27 KIP 1 non-phospho peptide (Lane 2) using Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] ab62364).
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