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AB75908

Anti-p27 KIP 1 (phospho T187) antibody

3

(1 Review)

|

(16 Publications)

Rabbit Polyclonal p27 KIP 1 phospho T187 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human CDKN1B pT187 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho T187) antibody (AB75908)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho T187) antibody (AB75908)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75908 (left) at 1/50 dilution or the same antibody preincubated with blocking peptide (right).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CDKN1B pT187 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P46527

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab75908 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed : 28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
See full target information CDKN1B pT187
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:1414-1441 PubMed39875724

2025

Micropeptide hSPAR regulates glutamine levels and suppresses mammary tumor growth via a TRIM21-P27KIP1-mTOR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Huang,Hua Lu,Yao Liu,Jiabei Wang,Qingan Xia,Xiangmin Shi,Yan Jin,Xiaolin Liang,Wei Wang,Xiaopeng Ma,Yangyi Wang,Meng Gong,Canjun Li,Chunlei Cang,Qinghua Cui,Ceshi Chen,Tao Shen,Lianxin Liu,Xiangting Wang

Experimental and therapeutic medicine 22:1425 PubMed34707706

2021

miR-142-5p regulates lipopolysaccharide-induced bovine epithelial cell proliferation and apoptosis via targeting BAG5.

Applications

Unspecified application

Species

Unspecified reactive species

Jinye Lu,Beibei Gu,Wei Lu,Jing Liu,Jiang Lu

Journal of translational medicine 19:314 PubMed34284793

2021

Hsa_circ_0000520 overexpression increases CDK2 expression via miR-1296 to facilitate cervical cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Qingling Zheng,Jin Zhang,Ting Zhang,Yanxiang Liu,Xiuluan Du,Xin Dai,Donghua Gu

Cell transplantation 29:963689720950226 PubMed32841050

2020

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase Suppresses Neuronal Apoptosis by Increasing Glycolysis and "cyclin-dependent kinase 1-Mediated Phosphorylation of p27 After Traumatic Spinal Cord Injury in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Liansheng Gao,Chun Wang,Bing Qin,Tao Li,Weilin Xu,Cameron Lenahan,Guangyu Ying,Jianru Li,Tengfei Zhao,Yongjian Zhu,Gao Chen

Aging 12:12812-12840 PubMed32633726

2020

PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Kun Wu,Chang Liu,Xin-Xing Li,Wei Ji,Chen-De Xin,Zhi-Qian Hu,Lin Zhou

Oncology letters 19:606-614 PubMed31897176

2020

miR-30a-3p inhibits the proliferation of liver cancer cells by targeting DNMT3a through the PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Chen,Yuan Gao,Qin Yu,Feng Tang,Pei-Wei Zhao,Su-Kun Luo,Ju-Sheng Lin,Hong Mei

Nature 569:423-427 PubMed31043741

2019

RB constrains lineage fidelity and multiple stages of tumour progression and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

David M Walter,Travis J Yates,Miguel Ruiz-Torres,Caroline Kim-Kiselak,A Andrea Gudiel,Charuhas Deshpande,Walter Z Wang,Michelle Cicchini,Kate L Stokes,John W Tobias,Elizabeth Buza,David M Feldser

Scientific reports 9:6688 PubMed31040372

2019

Reduced Basal Nitric Oxide Production Induces Precancerous Mammary Lesions via ERBB2 and TGFβ.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Ren,Xunzhen Zheng,Matthew Bommarito,Samantha Metzger,Yashna Walia,Joshua Letson,Allen Schroering,Andrea Kalinoski,David Weaver,Christopher Figy,Kam Yeung,Saori Furuta

PLoS biology 16:e2004408 PubMed29927970

2018

CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine.

Applications

Unspecified application

Species

Unspecified reactive species

Niloofar Ale-Agha,Christine Goy,Philipp Jakobs,Ioakim Spyridopoulos,Stefanie Gonnissen,Nadine Dyballa-Rukes,Karin Aufenvenne,Florian von Ameln,Mark Zurek,Tim Spannbrucker,Olaf Eckermann,Sascha Jakob,Simone Gorressen,Marcel Abrams,Maria Grandoch,Jens W Fischer,Karl Köhrer,René Deenen,Klaus Unfried,Joachim Altschmied,Judith Haendeler

Molecular medicine reports 18:1345-1352 PubMed29901196

2018

Telomerase reverse transcriptase induced thyroid carcinoma cell proliferation through PTEN/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Ning Hu
View all publications
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