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AB64949

Anti-p27 KIP 1 (phospho T198) antibody

5

(3 Reviews)

|

(8 Publications)

Rabbit Polyclonal p27 KIP 1 phospho T198 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CDKN1B pT198.

View Alternative Names

KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

2 Images
Western blot - Anti-p27 KIP 1 (phospho T198) antibody (AB64949)
  • WB

AbReview43711****

Western blot - Anti-p27 KIP 1 (phospho T198) antibody (AB64949)

All lanes:

Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 40 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 34 kDa

true

Exposure time: 1s

This image is courtesy of an anonymous Abreview

Western blot - Anti-p27 KIP 1 (phospho T198) antibody (AB64949)
  • WB

Unknown

Western blot - Anti-p27 KIP 1 (phospho T198) antibody (AB64949)

All lanes:

Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949) at 1/500 dilution

Lane 1:

HeLa cell extract at 30 µg

Lane 2:

HepG2 cell extract at 30 µg

Lane 3:

COLO205 cell extract at 30 µg

Lane 4:

COLO205 cell extract at 30 µg with immunising peptide

Predicted band size: 22 kDa

Observed band size: 28 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CDKN1B pT198. The exact immunogen used to generate this antibody is proprietary information.

P46527

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody againstnon phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed : 28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
See full target information CDKN1B pT198
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 33:450-462.e10 PubMed29533785

2018

Systematic Functional Annotation of Somatic Mutations in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Kwok-Shing Ng,Jun Li,Kang Jin Jeong,Shan Shao,Hu Chen,Yiu Huen Tsang,Sohini Sengupta,Zixing Wang,Venkata Hemanjani Bhavana,Richard Tran,Stephanie Soewito,Darlan Conterno Minussi,Daniela Moreno,Kathleen Kong,Turgut Dogruluk,Hengyu Lu,Jianjiong Gao,Collin Tokheim,Daniel Cui Zhou,Amber M Johnson,Jia Zeng,Carman Ka Man Ip,Zhenlin Ju,Matthew Wester,Shuangxing Yu,Yongsheng Li,Christopher P Vellano,Nikolaus Schultz,Rachel Karchin,Li Ding,Yiling Lu,Lydia Wai Ting Cheung,Ken Chen,Kenna R Shaw,Funda Meric-Bernstam,Kenneth L Scott,Song Yi,Nidhi Sahni,Han Liang,Gordon B Mills

eNeuro 4: PubMed28955722

2017

The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent.

Applications

Unspecified application

Species

Unspecified reactive species

Kalpana D Acharya,Sabin A Nettles,Katherine J Sellers,Dana D Im,Moriah Harling,Cassandra Pattanayak,Didem Vardar-Ulu,Cheryl F Lichti,Shixia Huang,Dean P Edwards,Deepak P Srivastava,Larry Denner,Marc J Tetel

Cell systems 4:73-83.e10 PubMed28017544

2016

Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Steven M Hill,Nicole K Nesser,Katie Johnson-Camacho,Mara Jeffress,Aimee Johnson,Chris Boniface,Simon E F Spencer,Yiling Lu,Laura M Heiser,Yancey Lawrence,Nupur T Pande,James E Korkola,Joe W Gray,Gordon B Mills,Sach Mukherjee,Paul T Spellman

Nature communications 7:12991 PubMed27694828

2016

Comprehensive functional analysis of the tousled-like kinase 2 frequently amplified in aggressive luminal breast cancers.

Applications

WB

Species

Unspecified reactive species

Jin-Ah Kim,Ying Tan,Xian Wang,Xixi Cao,Jamunarani Veeraraghavan,Yulong Liang,Dean P Edwards,Shixia Huang,Xuewen Pan,Kaiyi Li,Rachel Schiff,Xiao-Song Wang

Proceedings of the National Academy of Sciences of 113:6955-60 PubMed27274057

2016

Musashi-2 (MSI2) supports TGF-β signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander E Kudinov,Alexander Deneka,Anna S Nikonova,Tim N Beck,Young-Ho Ahn,Xin Liu,Cathleen F Martinez,Fred A Schultz,Samuel Reynolds,Dong-Hua Yang,Kathy Q Cai,Khaled M Yaghmour,Karmel A Baker,Brian L Egleston,Emmanuelle Nicolas,Adaeze Chikwem,Gregory Andrianov,Shelly Singh,Hossein Borghaei,Ilya G Serebriiskii,Don L Gibbons,Jonathan M Kurie,Erica A Golemis,Yanis Boumber

Autophagy 10:1241-55 PubMed24905460

2014

Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liu,Jingyi Li,Tao Zhang,Linzhi Zou,Yi Chen,Kui Wang,Yunlong Lei,Kefei Yuan,Yi Li,Jiang Lan,Lin Cheng,Na Xie,Rong Xiang,Edouard C Nice,Canhua Huang,Yuquan Wei

Biochimica et biophysica acta 1842:945-58 PubMed24583340

2014

UCH-L1 induces podocyte hypertrophy in membranous nephropathy by protein accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Frithjof Lohmann,Marlies Sachs,Tobias N Meyer,Henning Sievert,Maja T Lindenmeyer,Thorsten Wiech,Clemens D Cohen,Stefan Balabanov,R A K Stahl,Catherine Meyer-Schwesinger

The Journal of thoracic and cardiovascular surgery 145:531-8 PubMed23142123

2012

Dasatinib, a small molecule inhibitor of the Src kinase, reduces the growth and activates apoptosis in pre-neoplastic Barrett's esophagus cell lines: evidence for a noninvasive treatment of high-grade dysplasia.

Applications

Unspecified application

Species

Human

Landon J Inge,Aaron J Fowler,Kimberly M Paquette,Amanda L Richer,Nhan Tran,Ross M Bremner
View all publications
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