Anti-P2RY1 antibody [HL2702] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (AB325869)

ab325869 detects P2Y1 protein by immunohistochemical analysis. Sample : Paraffin-embedded human pancreas. P2Y1 stained by ab325869 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

Supplier Data

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (AB325869)

Boiled and unboiled LoVo whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325869 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (ab325869) at 1/500 dilution

Lane 1:

Boiled LoVo whole cell extracts at 30 µg

Lane 2:

Boiled LoVo membrane extracts at 30 µg

Lane 3:

Unboiled LoVo whole cell extracts at 30 µg

Lane 4:

Unboiled LoVo membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (AB325869)

Boiled and unboiled LoVo whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325869 diluted at 1 : 1000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (ab325869) at 1/1000 dilution

Lane 1:

Boiled LoVo whole cell extracts at 30 µg

Lane 2:

Boiled LoVo membrane extracts at 30 µg

Lane 3:

Unboiled LoVo whole cell extracts at 30 µg

Lane 4:

Unboiled LoVo membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (AB325869)

Unboiled non-transfected (–) and transfected (+) 293T and Expi293 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325869 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (ab325869) at 1/5000 dilution

Lanes 1 and 9:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled P2RY1 transfected (+) 293T whole cell extracts at 30 µg

Lanes 3, 5 and 7:

Unboiled non-transfected (–) Expi293 whole cell extracts at 30 µg

Lane 4:

Unboiled P2RY2 transfected (+) Expi293 whole cell extracts at 30 µg

Lane 6:

Unboiled P2RY11 transfected (+) Expi293 whole cell extracts at 30 µg

Lane 8:

Unboiled P2RY12 transfected (+) Expi293 whole cell extracts at 30 µg

Lane 10:

Unboiled P2RY13 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (AB325869)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325869 diluted at 1 : 50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (ab325869) at 1/50000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged P2RY1 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged P2RY1 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (AB325869)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325869 diluted at 1 : 5000 and competitor's antibody diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-P2RY1 antibody [HL2702] - BSA and Azide free (ab325869) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged P2RY1 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged P2RY1 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true