Anti-P2RY2 antibody [HL2704] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (AB325870)

ab325870 detects P2Y2 protein at cell membrane by immunofluorescent analysis. Sample : THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : P2Y2 stained by ab325870 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (AB325870)

Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325870 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE : whole cell extract; ME : membrane extract)

All lanes:

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (ab325870) at 1/1000 dilution

Lane 1:

Boiled HL-60 whole cell extracts at 30 µg

Lane 2:

Boiled HL-60 membrane extracts at 30 µg

Lane 3:

Unboiled HL-60 whole cell extracts at 30 µg

Lane 4:

Unboiled HL-60 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (AB325870)

Untreated (–) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted withab325870 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (ab325870) at 1/500 dilution

Lane 1:

Untreated (–) and unboiled HL-60 whole cell extracts at 30 µg

Lane 2:

PNGase F, 2 hr treated (+) unboiled HL-60 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (AB325870)

Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325870 diluted at 1 : 1000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (ab325870) at 1/1000 dilution

Lane 1:

Boiled HL-60 whole cell extracts at 30 µg

Lane 2:

Boiled HL-60 membrane extracts at 30 µg

Lane 3:

Unboiled HL-60 whole cell extracts at 30 µg

Lane 4:

Unboiled HL-60 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (AB325870)

Non-transfected (–) and transfected (+) unboiled 293T and Expi293 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325870 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (ab325870) at 1/5000 dilution

Lanes 1 and 9:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 2:

P2RY1 transfected (+) unboiled 293T whole cell extracts at 30 µg

Lanes 3, 5 and 7:

Non-transfected (–) unboiled Expi293 whole cell extracts at 30 µg

Lane 4:

P2RY2 transfected (+) unboiled 293T whole cell extracts at 30 µg

Lane 6:

P2RY11 transfected (+) unboiled 293T whole cell extracts at 30 µg

Lane 8:

P2RY12 transfected (+) unboiled 293T whole cell extracts at 30 µg

Lane 10:

P2RY13 transfected (+) unboiled Expi293 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (AB325870)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325870 diluted at 1 : 5000 and competitor's antibody diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (ab325870) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged P2RY2 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged P2RY2 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (AB325870)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ab325870 diluted at 1 : 20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-P2RY2 antibody [HL2704] - BSA and Azide free (ab325870) at 1/20000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged P2RY2 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged P2RY2 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false