Anti-P2X3 antibody [EPR26568-3]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)

Immunohistochemical analysis of paraffin-embedded Human dorsal root ga tissue labeling P2X3 with ab300493 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human dorsal root ganglion. The section was incubated with ab300493 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse dorsal root ganglion (fresh) tissue labeling P2X3 with ab300493 at 1/50 (9.96 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse dorsal root ganglion is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)

Immunohistochemical analysis of paraffin-embedded Mouse dorsal root ga tissue labeling P2X3 with ab300493 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse dorsal root ganglion. The section was incubated with ab300493 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)

Immunohistochemical analysis of paraffin-embedded Rat dorsal root gang tissue labeling P2X3 with ab300493 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on rat dorsal root ganglion. The section was incubated with ab300493 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat dorsal root ganglion (fresh) tissue labeling P2X3 with ab300493 at 1/50 (9.96 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat dorsal root ganglion is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

• IP

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Immunoprecipitation - Anti-P2X3 antibody [EPR26568-3] (AB300493)

P2X3 was immunoprecipitated from 0.35 mg Rat dorsal ganglion tissue lysate 10 ug with ab300493 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300493 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Rat dorsal ganglion tissue lysate 10 ug

Lane 2 : ab300493 IP in Rat dorsal ganglion tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300493 in rat dorsal ganglion tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

Lanes 1 - 3:

Immunoprecipitation - Anti-P2X3 antibody [EPR26568-3] (ab300493) at 1/1000 dilution

Lanes 1 - 3:

Immunoprecipitation - Anti-P2X3 antibody [EPR26568-3] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/p2x3-antibody-epr26568-3-bsa-and-azide-free-ab300494'>ab300494</a>)

All lanes:

Rat dorsal ganglion tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3min

• WB

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Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-P2X3 antibody [EPR26568-3] (ab300493) at 1/1000 dilution

All lanes:

Human heart tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-P2X3 antibody [EPR26568-3] (ab300493) at 1/1000 dilution

Lane 1:

Rat dorsal galion tissue lysate 20

Lane 2:

Rat heart tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-P2X3 antibody [EPR26568-3] (ab300493) at 1/1000 dilution

All lanes:

Mouse dorsal galion tissue lysate 20

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa

false

Exposure time: 3min