AB134559

Anti-P2X4 antibody

Name: Anti-P2X4 antibody (ab134559) | Abcam
Brand: Abcam Limited
SKU: AB134559
Price: 535 USD
Availability: InStock

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(6 Publications)

Goat Polyclonal P2X4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Mouse P2rx4 aa 50-100.

View Alternative Names

P2X purinoceptor 4, P2X4, ATP receptor, Purinergic receptor, P2RX4

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (AB134559)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (AB134559)

Immunofluorescence analysis of paraformaldehyde fixed A431 cells labeling P2X4 with ab134559. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (4 μg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (4 μg/mL).

Supplier Data

Western blot - Anti-P2X4 antibody (AB134559)

All lanes:

Western blot - Anti-P2X4 antibody (ab134559) at 0.3 µg/mL

All lanes:

A549 (human lung carcinoma cell line) cell lysate (35µg protein in RIPA buffer)

Predicted band size: 34 kDa,43 kDa

Observed band size: 48-50 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Mouse P2rx4 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

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Specificity

Preliminary testing was unsuccessful on Mouse and Rat Kidney and Liver in Western blot.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The P2X4 receptor also known as P2RX4 is an ATP-gated ion channel that functions as a non-selective cation channel integral to numerous physiological processes. This receptor has a molecular mass of approximately 46 kDa. You can find it expressed highly in the central nervous system specifically in microglial cells and also in various peripheral tissues such as the heart and vascular endothelial cells. The channel allows for the rapid influx of calcium sodium and potassium ions upon activation by extracellular ATP.

Biological function summary

P2X4 receptors play important roles in modulating synaptic transmission and plasticity particularly through their involvement in maintaining calcium homeostasis. These receptors do not function in isolation; they often operate as part of larger protein complexes including potential interactions with other P2X receptors such as P2X7. Through these associations P2X4 influences inflammatory responses and neuropathic pain acting as a bridge between extracellular signals and intracellular processes.

Pathways

P2X4 is important in the regulation of several signaling cascades notably the inflammatory and pain pathways. For example its interaction with the NF-kB signaling pathway highlights its contribution to inflammatory responses. P2X4 also interacts with proteins like pannexin-1 forming conduits that may amplify ATP release an important step in the propagation of inflammatory signals contributing to long-term changes in synaptic function and plasticity.

P2X4 receptor activity links to various conditions particularly neuropathic pain and heart disease. Alterations in P2X4 expression or function can contribute to the development of chronic pain conditions where enhanced P2X4 signaling is observed in microglial cells in response to nerve injury. In cardiac settings changes in P2X4 may influence heart failure with potential interactions noted with P2X7 receptors; these interactions can modulate inflammatory pathways that exacerbate cardiac dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium (PubMed : 9016352). CTP, but not GTP or UTP, functions as a weak affinity agonist for P2RX4 (By similarity). Activated by extracellularly released ATP, it plays multiple role in immunity and central nervous system physiology (PubMed : 35165166). Plays a key role in initial steps of T-cell activation and Ca(2+) microdomain formation (By similarity). Participates also in basal T-cell activity without TCR/CD3 stimulation (By similarity). Promotes the differentiation and activation of Th17 cells via expression of retinoic acid-related orphan receptor C/RORC (PubMed : 35165166). Upon activation, drives microglia motility via the PI3K/Akt pathway (By similarity). Could also function as an ATP-gated cation channel of lysosomal membranes (By similarity).

See full target information P2RX4

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Purinergic signalling 21:565-576 PubMed40560521

2025

Electroacupuncture alleviates capsaicin-induced rectal visceral pain in rats via inhibiting TRPV1 expression by blocking the P2X4R-activated p38 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yahong Xue,Qinbing Zhu,Jing Zhang,Xiaofeng Wang

Journal of neurovirology 29:241-251 PubMed37166584

2023

Expression in skin biopsies supports genetic evidence linking CAMKK2, P2X7R and P2X4R with HIV-associated sensory neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Gaff,Fitri Octaviana,Connie Jackaman,Peter Kamerman,John Papadimitriou,Silvia Lee,Jenjira Mountford,Patricia Price

International journal of molecular sciences 22: PubMed34769029

2021

Involvement of the miR-363-5p/P2RX4 Axis in Regulating Schwann Cell Phenotype after Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jung Sohn,Yun-Kyeong Nam,Hwan-Tae Park

Experimental and molecular pathology 120:104636 PubMed33838119

2021

Immunohistochemical evidence of P2X7R, P2X4R and CaMKK2 in pyramidal neurons of frontal cortex does not align with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Gaff,Connie Jackaman,John Papadimitriou,Shelley Waters,Catriona McLean,Patricia Price

Purinergic signalling 15:367-374 PubMed31401785

2019

Role of purinergic receptors in hepatobiliary carcinoma in Pakistani population: an approach towards proinflammatory role of P2X4 and P2X7 receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Arun Asif,Madiha Khalid,Sobia Manzoor,Hassam Ahmad,Aman Ur Rehman

PloS one 7:e47985 PubMed23110151

2012

Antibody phage display assisted identification of junction plakoglobin as a potential biomarker for atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Seraina Cooksley-Decasper,Hans Reiser,Daniela S Thommen,Barbara Biedermann,Michel Neidhart,Joanna Gawinecka,Gieri Cathomas,Fabian C Franzeck,Christophe Wyss,Roland Klingenberg,Paolo Nanni,Bernd Roschitzki,Christian Matter,Petra Wolint,Maximilian Y Emmert,Marc Husmann,Beatrice Amann-Vesti,Wilibald Maier,Steffen Gay,Thomas F Lüscher,Arnold von Eckardstein,Danielle Hof

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P2X4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (AB134559)

Western blot - Anti-P2X4 antibody (AB134559)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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