AB243734

Anti-P2X4 antibody

Name: Anti-P2X4 antibody (ab243734) | Abcam
Brand: Abcam Limited
SKU: AB243734

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(2 Publications )

Rabbit Polyclonal P2X4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P2RX4 aa 350-400.

View Alternative Names

P2X purinoceptor 4, P2X4, ATP receptor, Purinergic receptor, P2RX4

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (AB243734)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (AB243734)

PFA fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling P2X4 using ab243734 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis. No membranous positivity in myocytes, as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Formalin-fixed, paraffin-embedded human placenta tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-P2X4 antibody (AB243734)

All lanes:

Western blot - Anti-P2X4 antibody (ab243734) at 0.4 µg/mL

All lanes:

SK-MEL-30 cell lysate

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human P2RX4 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

Q99571

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The P2X4 receptor also known as P2RX4 is an ATP-gated ion channel that functions as a non-selective cation channel integral to numerous physiological processes. This receptor has a molecular mass of approximately 46 kDa. You can find it expressed highly in the central nervous system specifically in microglial cells and also in various peripheral tissues such as the heart and vascular endothelial cells. The channel allows for the rapid influx of calcium sodium and potassium ions upon activation by extracellular ATP.

Biological function summary

P2X4 receptors play important roles in modulating synaptic transmission and plasticity particularly through their involvement in maintaining calcium homeostasis. These receptors do not function in isolation; they often operate as part of larger protein complexes including potential interactions with other P2X receptors such as P2X7. Through these associations P2X4 influences inflammatory responses and neuropathic pain acting as a bridge between extracellular signals and intracellular processes.

Pathways

P2X4 is important in the regulation of several signaling cascades notably the inflammatory and pain pathways. For example its interaction with the NF-kB signaling pathway highlights its contribution to inflammatory responses. P2X4 also interacts with proteins like pannexin-1 forming conduits that may amplify ATP release an important step in the propagation of inflammatory signals contributing to long-term changes in synaptic function and plasticity.

P2X4 receptor activity links to various conditions particularly neuropathic pain and heart disease. Alterations in P2X4 expression or function can contribute to the development of chronic pain conditions where enhanced P2X4 signaling is observed in microglial cells in response to nerve injury. In cardiac settings changes in P2X4 may influence heart failure with potential interactions noted with P2X7 receptors; these interactions can modulate inflammatory pathways that exacerbate cardiac dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium (PubMed : 9016352). CTP, but not GTP or UTP, functions as a weak affinity agonist for P2RX4 (By similarity). Activated by extracellularly released ATP, it plays multiple role in immunity and central nervous system physiology (PubMed : 35165166). Plays a key role in initial steps of T-cell activation and Ca(2+) microdomain formation (By similarity). Also participates in basal T-cell activity without TCR/CD3 stimulation (By similarity). Promotes the differentiation and activation of Th17 cells via expression of retinoic acid-related orphan receptor C/RORC (PubMed : 35165166). Upon activation, drives microglia motility via the PI3K/Akt pathway (By similarity). Could also function as an ATP-gated cation channel of lysosomal membranes (By similarity).

See full target information P2RX4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of reproductive immunology (New York, N.Y. : 1989) 94:e70132 PubMed40815040

2025

ATP/P2X4 Regulates Inflammation and Oxidative Stress in Endometriosis Through NLRP3 Inflammasome-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Wu,Yan Guo

Purinergic signalling 16:529-542 PubMed33025427

2020

The P2X7 ion channel is dispensable for energy and metabolic homeostasis of white and brown adipose tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian,Markus Heine,Ioannis Evangelakos,Michelle Y Jaeckstein,Nicola Schaltenberg,Tobias Stähler,Friedrich Koch-Nolte,Manju Kumari,Joerg Heeren

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-P2X4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (AB243734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Western blot - Anti-P2X4 antibody (AB243734)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise