Rabbit Polyclonal P2X7 antibody. Suitable for IHC-P, WB and reacts with Rat samples. Cited in 9 publications.
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- Molecular vision 28:500-5062022Epac1 and PKA agonists inhibit ROS to reduce NLRP3 inflammasome proteins in retinal endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Liu,Youde Jiang,Jena J SteinlePubMed 37089701
- Experimental and therapeutic medicine 23:1612022P2X7R antagonist protects against renal injury in mice with adriamycin nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanji Zhu,Min Liu,Wenlong Xun,Keliang Li,Xiangji NiuPubMed 35069842
- Cell death discovery 7:3462021Moderate-intensity exercise alleviates pyroptosis by promoting autophagy in osteoarthritis via the P2X7/AMPK/mTOR axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZihao Li,Ziyu Huang,He Zhang,Jinghan Lu,Yicheng Tian,Shang Piao,Zhiming Lin,Lunhao BaiPubMed 34759265
- Molecular neurobiology 58:4226-42362021BDNF Participates in Chronic Constriction Injury-Induced Neuropathic Pain via Transcriptionally Activating P2X in Primary Sensory Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Wu,Zhiwen Shen,Hui Xu,Kun Zhang,Mingyan Guo,Fei Wang,Junhua LiPubMed 33963520
- Neuroscience bulletin 37:641-6562021Paraventricular Nucleus P2X7 Receptors Aggravate Acute Myocardial Infarction Injury via ROS-Induced Vasopressin-V1b Activation in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Cheng,Yinggang Sun,Qin Wu,Kokwin Ooi,Yi Feng,Chunmei Xia,Danian ZhuPubMed 33620697
- American journal of translational research 12:45-532020The P2X7 receptor (P2X7R)-specific antagonist A804598 inhibits inflammatory reaction in human fibroblast-like synoviocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Liu et. al.PubMed 32051736
- Molecular medicine reports 20:5084-50902019High glucose inhibits osteogenic differentiation and proliferation of MC3T3‑E1 cells by regulating P2X7.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinsan Yang et. al.PubMed 31702818
- General physiology and biophysics 38:407-4162019Involvement of P2X7 receptors in satellite glial cells of dorsal root ganglia in the BmK I -induced pain model of rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Zhou,Danting Feng,Xiaoxue Zhang,Chenchen Xia,Zhiping Zhang,Jiahao Kang,Zhiyong Tan,Bin WuPubMed 31595882
- Neuroscience bulletin 35:624-6362019Up-regulation of P2X7 Receptors Contributes to Spinal Microglial Activation and the Development of Pain Induced by BmK-I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Zhou et. al.PubMed 30820829
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Mouse | Predicted | Predicted |
| Rat | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
1 product for Alternative Version
Target data
Function
Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
Alternative names
P2X purinoceptor 7, P2X7, ATP receptor, P2Z receptor, Purinergic receptor, P2rx7
Recommended products
Rabbit Polyclonal P2X7 antibody. Suitable for IHC-P, WB and reacts with Rat samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The P2X7 receptor also known as P2RX7 is a ligand-gated ion channel primarily activated by ATP. It possesses a mass of approximately 68 kDa and is mainly expressed in immune cells like macrophages and microglia. P2X7 functions as a trimer forming a pore in the cell membrane that allows the passage of ions such as calcium (Ca²⁺) and sodium (Na⁺) influencing cell signaling and survival. This receptor is notable for its unique ability to change permeability to larger molecules upon prolonged stimulation with ATP.
- Biological function summary
The P2X7 receptor influences immune response and inflammation. It plays a role in the release of pro-inflammatory cytokines like interleukin-1β (IL-1β) therefore participating in the regulation of inflammatory and immune responses. It forms a complex with other purinergic receptors affecting processes such as apoptosis and cell proliferation. The activation of P2X7 can lead to the formation of the inflammasome a component critical for cytokine processing and release.
- Pathways
The P2X7 receptor integrates into the NOD-like receptor (NLR) signaling and the purinergic signaling pathways. It interacts with proteins from these pathways including NLRP3 and pannexin-1 which contribute to inflammasome assembly and function. P2X7 also impacts intracellular signaling cascades such as the PI3K/AKT pathway influencing cellular survival and immune responses.
- Associated diseases and disorders
Aberrant P2X7 receptor activity has connections to inflammatory and neurodegenerative conditions. In chronic inflammation the enhanced activity of this receptor can exacerbate diseases like rheumatoid arthritis by promoting excessive cytokine release. It is also associated with neurodegenerative disorders like Alzheimer's disease where P2X7 influences neuroinflammation and neuronal death interacting with proteins such as amyloid-beta.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-P2X7 antibody (ab109054)
All lanes: Western blot - Anti-P2X7 antibody (ab109054) at 1 µg/mL
All lanes: Rat Hippocampus Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 20 kDa, 68 kDa, 78 kDa
Exposure time: 90s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody (ab109054)
IHC image of ab109054 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109054, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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