AB109054

Anti-P2X7 antibody

Name: Anti-P2X7 antibody (ab109054) | Abcam
Brand: Abcam Limited
SKU: AB109054
Price: 415 USD
Availability: InStock
Rating: 1 (1 reviews)

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What is this?

(1 Review)

(15 Publications)

Rabbit Polyclonal P2X7 antibody. Suitable for IHC-P, WB and reacts with Rat samples. Cited in 15 publications.

View Alternative Names

P2X purinoceptor 7, P2X7, ATP receptor, P2Z receptor, Purinergic receptor, P2rx7

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody (AB109054)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody (AB109054)

IHC image of ab109054 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109054, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-P2X7 antibody (AB109054)

All lanes:

Western blot - Anti-P2X7 antibody (ab109054) at 1 µg/mL

All lanes:

Rat Hippocampus Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 69 kDa

Observed band size: 20 kDa,68 kDa,78 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.1 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The P2X7 receptor also known as P2RX7 is a ligand-gated ion channel primarily activated by ATP. It possesses a mass of approximately 68 kDa and is mainly expressed in immune cells like macrophages and microglia. P2X7 functions as a trimer forming a pore in the cell membrane that allows the passage of ions such as calcium (Ca²⁺) and sodium (Na⁺) influencing cell signaling and survival. This receptor is notable for its unique ability to change permeability to larger molecules upon prolonged stimulation with ATP.

Biological function summary

The P2X7 receptor influences immune response and inflammation. It plays a role in the release of pro-inflammatory cytokines like interleukin-1β (IL-1β) therefore participating in the regulation of inflammatory and immune responses. It forms a complex with other purinergic receptors affecting processes such as apoptosis and cell proliferation. The activation of P2X7 can lead to the formation of the inflammasome a component critical for cytokine processing and release.

Pathways

The P2X7 receptor integrates into the NOD-like receptor (NLR) signaling and the purinergic signaling pathways. It interacts with proteins from these pathways including NLRP3 and pannexin-1 which contribute to inflammasome assembly and function. P2X7 also impacts intracellular signaling cascades such as the PI3K/AKT pathway influencing cellular survival and immune responses.

Aberrant P2X7 receptor activity has connections to inflammatory and neurodegenerative conditions. In chronic inflammation the enhanced activity of this receptor can exacerbate diseases like rheumatoid arthritis by promoting excessive cytokine release. It is also associated with neurodegenerative disorders like Alzheimer's disease where P2X7 influences neuroinflammation and neuronal death interacting with proteins such as amyloid-beta.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.

See full target information P2rx7

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1598151 PubMed40520155

2025

ATP/P2X7 receptor/NLRP3 pathway facilitates renal tubular epithelial-myofibroblast transdifferentiation and interstitial fibrosis in rats with unilateral ureteral obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Tan,Feiyan Li,Yanchao Cui,Ziwen Li,Shiqiang Yan,Quanfeng Deng

Scientific reports 15:965 PubMed39762398

2025

Role of P × receptor during focused ultrasound induced blood brain barrier modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Junwon Park,Young Cheol Na,Jihyeon Lee,Younghee Seo,Hojin Kim,Sangheon Han,Byeong-Wook Song,Won Seok Chang

Cellular and molecular life sciences : CMLS 81:444 PubMed39485512

2024

Transcriptomic alterations in APP/PS1 mice astrocytes lead to early postnatal axon initial segment structural changes.

Applications

Unspecified application

Species

Unspecified reactive species

María José Benitez,Diana Retana,Lara Ordoñez-Gutiérrez,Inés Colmena,María José Goméz,Rebeca Álvarez,María Ciorraga,Ana Dopazo,Francisco Wandosell,Juan José Garrido

The journal of headache and pain 25:120 PubMed39044141

2024

The effect of P2X7 antagonism on subcortical spread of optogenetically-triggered cortical spreading depression and neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Burak Uzay,Buket Donmez-Demir,Sinem Yilmaz Ozcan,Emine Eren Kocak,Muge Yemisci,Yasemin Gursoy Ozdemir,Turgay Dalkara,Hulya Karatas

Cell stress & chaperones 29:440-455 PubMed38653383

2024

Involvement of oxidative stress-AMPK-Cx43-NLRP3 pathway in extracellular matrix remodeling of gastric smooth muscle cells in rats with diabetic gastroparesis.

Applications

Unspecified application

Species

Unspecified reactive species

Baihui Song,Gaoyuan Zhang,Yitegele Bao,Mohan Zhang

Molecular vision 28:500-506 PubMed37089701

2022

Epac1 and PKA agonists inhibit ROS to reduce NLRP3 inflammasome proteins in retinal endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Youde Jiang,Jena J Steinle

Experimental and therapeutic medicine 23:161 PubMed35069842

2022

P2X7R antagonist protects against renal injury in mice with adriamycin nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yanji Zhu,Min Liu,Wenlong Xun,Keliang Li,Xiangji Niu

Cell death discovery 7:346 PubMed34759265

2021

Moderate-intensity exercise alleviates pyroptosis by promoting autophagy in osteoarthritis via the P2X7/AMPK/mTOR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zihao Li,Ziyu Huang,He Zhang,Jinghan Lu,Yicheng Tian,Shang Piao,Zhiming Lin,Lunhao Bai

Frontiers in cell and developmental biology 9:695041 PubMed34222263

2021

IRE1-mTOR-PERK Axis Coordinates Autophagy and ER Stress-Apoptosis Induced by P2X7-Mediated Ca Influx in Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Zihao Li,Ziyu Huang,He Zhang,Jinghan Lu,Yingliang Wei,Yue Yang,Lunhao Bai

Molecular neurobiology 58:4226-4236 PubMed33963520

2021

BDNF Participates in Chronic Constriction Injury-Induced Neuropathic Pain via Transcriptionally Activating P2X in Primary Sensory Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wu,Zhiwen Shen,Hui Xu,Kun Zhang,Mingyan Guo,Fei Wang,Junhua Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P2X7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody (AB109054)

Western blot - Anti-P2X7 antibody (AB109054)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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