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AB233527

Anti-P2X7 antibody

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(1 Publication)

Rabbit Polyclonal P2X7 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human P2RX7 aa 1 to C-terminus.

View Alternative Names

P2X purinoceptor 7, P2X7, ATP receptor, P2Z receptor, Purinergic receptor, P2RX7

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody (AB233527)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody (AB233527)

Formalin-fixed, paraffin-embedded human kidney tissue stained for P2X7 using ab233527 at 20 μg/ml in immunohistochemical analysis.

Western blot - Anti-P2X7 antibody (AB233527)
  • WB

Supplier Data

Western blot - Anti-P2X7 antibody (AB233527)

All lanes:

Western blot - Anti-P2X7 antibody (ab233527) at 3 µg/mL

All lanes:

Recombinant human P2X7 protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 69 kDa

false

Western blot - Anti-P2X7 antibody (AB233527)
  • WB

Supplier Data

Western blot - Anti-P2X7 antibody (AB233527)

All lanes:

Western blot - Anti-P2X7 antibody (ab233527) at 3 µg/mL

Lane 1:

Pig brain tissue lysate

Lane 2:

Pig stomach tissue lysate

Lane 3:

Pig heart tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human P2RX7 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99572

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The P2X7 receptor also known as P2RX7 is a ligand-gated ion channel primarily activated by ATP. It possesses a mass of approximately 68 kDa and is mainly expressed in immune cells like macrophages and microglia. P2X7 functions as a trimer forming a pore in the cell membrane that allows the passage of ions such as calcium (Ca²⁺) and sodium (Na⁺) influencing cell signaling and survival. This receptor is notable for its unique ability to change permeability to larger molecules upon prolonged stimulation with ATP.
Biological function summary

The P2X7 receptor influences immune response and inflammation. It plays a role in the release of pro-inflammatory cytokines like interleukin-1β (IL-1β) therefore participating in the regulation of inflammatory and immune responses. It forms a complex with other purinergic receptors affecting processes such as apoptosis and cell proliferation. The activation of P2X7 can lead to the formation of the inflammasome a component critical for cytokine processing and release.

Pathways

The P2X7 receptor integrates into the NOD-like receptor (NLR) signaling and the purinergic signaling pathways. It interacts with proteins from these pathways including NLRP3 and pannexin-1 which contribute to inflammasome assembly and function. P2X7 also impacts intracellular signaling cascades such as the PI3K/AKT pathway influencing cellular survival and immune responses.

Aberrant P2X7 receptor activity has connections to inflammatory and neurodegenerative conditions. In chronic inflammation the enhanced activity of this receptor can exacerbate diseases like rheumatoid arthritis by promoting excessive cytokine release. It is also associated with neurodegenerative disorders like Alzheimer's disease where P2X7 influences neuroinflammation and neuronal death interacting with proteins such as amyloid-beta.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation (PubMed : 17483156, PubMed : 25281740, PubMed : 9038151). Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux (PubMed : 17483156, PubMed : 20453110, PubMed : 28235784, PubMed : 39262850). Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules (PubMed : 17483156). In microglia, may mediate NADPH transport across the plasma membrane (PubMed : 39142135). In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP. In microglial cells, P2RX7 activation leads to the release of pro-inflammatory cytokines, such as IL-1beta and IL-18, through the activation of the NLRP3 inflammasome and caspase-1 (PubMed : 26877061). Cooperates with KCNK6 to activate NLRP3 inflammasome (By similarity). Activates death pathways leading to apoptosis and autophagy (PubMed : 21821797, PubMed : 23303206, PubMed : 28326637). Activates death pathways leading to pyroptosis (By similarity).. Isoform B. Shows ion channel activity but no macropore function.. Isoform H. Non-functional channel.. Isoform J. Non-functional channel.
See full target information P2RX7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science (New York, N.Y.) 373: PubMed34437091

2021

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Hyun Han,Emily J Onufer,Li-Hao Huang,Robert W Sprung,W Sean Davidson,Rafael S Czepielewski,Mary Wohltmann,Mary G Sorci-Thomas,Brad W Warner,Gwendalyn J Randolph
View all publications
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Product promise

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