AB229453

Anti-P2X7 antibody - C-terminal

Name: Anti-P2X7 antibody - C-terminal (ab229453) | Abcam
Brand: Abcam Limited
SKU: AB229453
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal P2X7 antibody. C-terminal. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Rat P2rx7.

View Alternative Names

P2x7, P2rx7, P2X purinoceptor 7, P2X7, ATP receptor, P2Z receptor, Purinergic receptor

1 Images

Supplier Data

Western blot - Anti-P2X7 antibody - C-terminal (AB229453)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-P2X7 antibody - C-terminal (ab229453) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 69 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Rat P2rx7. The exact immunogen used to generate this antibody is proprietary information.

Q64663

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The P2X7 receptor also known as P2RX7 is a ligand-gated ion channel primarily activated by ATP. It possesses a mass of approximately 68 kDa and is mainly expressed in immune cells like macrophages and microglia. P2X7 functions as a trimer forming a pore in the cell membrane that allows the passage of ions such as calcium (Ca²⁺) and sodium (Na⁺) influencing cell signaling and survival. This receptor is notable for its unique ability to change permeability to larger molecules upon prolonged stimulation with ATP.

Biological function summary

The P2X7 receptor influences immune response and inflammation. It plays a role in the release of pro-inflammatory cytokines like interleukin-1β (IL-1β) therefore participating in the regulation of inflammatory and immune responses. It forms a complex with other purinergic receptors affecting processes such as apoptosis and cell proliferation. The activation of P2X7 can lead to the formation of the inflammasome a component critical for cytokine processing and release.

Pathways

The P2X7 receptor integrates into the NOD-like receptor (NLR) signaling and the purinergic signaling pathways. It interacts with proteins from these pathways including NLRP3 and pannexin-1 which contribute to inflammasome assembly and function. P2X7 also impacts intracellular signaling cascades such as the PI3K/AKT pathway influencing cellular survival and immune responses.

Aberrant P2X7 receptor activity has connections to inflammatory and neurodegenerative conditions. In chronic inflammation the enhanced activity of this receptor can exacerbate diseases like rheumatoid arthritis by promoting excessive cytokine release. It is also associated with neurodegenerative disorders like Alzheimer's disease where P2X7 influences neuroinflammation and neuronal death interacting with proteins such as amyloid-beta.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells.

See full target information P2rx7

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1589 PubMed39939598

2025

An optimised faecal microRNA sequencing pipeline reveals fibrosis in Trichuris muris infection.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Layton,Sian Goldsworthy,EnJun Yang,Wei Yee Ong,Tara E Sutherland,Allison J Bancroft,Seona Thompson,Veonice Bijin Au,Sam Griffiths-Jones,Richard K Grencis,Anna-Marie Fairhurst,Ian S Roberts

Purinergic signalling 16:529-542 PubMed33025427

2020

The P2X7 ion channel is dispensable for energy and metabolic homeostasis of white and brown adipose tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian,Markus Heine,Ioannis Evangelakos,Michelle Y Jaeckstein,Nicola Schaltenberg,Tobias Stähler,Friedrich Koch-Nolte,Manju Kumari,Joerg Heeren

International journal of molecular medicine 42:1152-1160 PubMed29750294

2018

LncRNA uc.48+ is involved in the diabetic immune and inflammatory responses mediated by P2X7 receptor in RAW264.7 macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Wu,Fang Wen,Mei Jiang,Qiang Liu,Yijun Nie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-P2X7 antibody - C-terminal

Western blot - Anti-P2X7 antibody - C-terminal (AB229453)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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