Rabbit Recombinant Monoclonal P2X7 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples.
Images
![Western blot - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--western-blot-img29130.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29821.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4180.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99414.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709)")
![Immunoprecipitation - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunoprecipitation-img11655.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (AB279709)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Tested | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells.
Alternative names
P2X purinoceptor 7, P2X7, ATP receptor, P2Z receptor, Purinergic receptor, P2rx7, P2x7
Recommended products
Rabbit Recombinant Monoclonal P2X7 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples.
Alternative names
P2X purinoceptor 7, P2X7, ATP receptor, P2Z receptor, Purinergic receptor, P2rx7, P2x7
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR24130-77
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab279709 is the carrier-free version of Anti-P2X7 antibody [EPR24130-77] ab259942.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
The P2X7 receptor also known as P2RX7 is a ligand-gated ion channel primarily activated by ATP. It possesses a mass of approximately 68 kDa and is mainly expressed in immune cells like macrophages and microglia. P2X7 functions as a trimer forming a pore in the cell membrane that allows the passage of ions such as calcium (Ca²⁺) and sodium (Na⁺) influencing cell signaling and survival. This receptor is notable for its unique ability to change permeability to larger molecules upon prolonged stimulation with ATP.
- Biological function summary
The P2X7 receptor influences immune response and inflammation. It plays a role in the release of pro-inflammatory cytokines like interleukin-1β (IL-1β) therefore participating in the regulation of inflammatory and immune responses. It forms a complex with other purinergic receptors affecting processes such as apoptosis and cell proliferation. The activation of P2X7 can lead to the formation of the inflammasome a component critical for cytokine processing and release.
- Pathways
The P2X7 receptor integrates into the NOD-like receptor (NLR) signaling and the purinergic signaling pathways. It interacts with proteins from these pathways including NLRP3 and pannexin-1 which contribute to inflammasome assembly and function. P2X7 also impacts intracellular signaling cascades such as the PI3K/AKT pathway influencing cellular survival and immune responses.
- Associated diseases and disorders
Aberrant P2X7 receptor activity has connections to inflammatory and neurodegenerative conditions. In chronic inflammation the enhanced activity of this receptor can exacerbate diseases like rheumatoid arthritis by promoting excessive cytokine release. It is also associated with neurodegenerative disorders like Alzheimer's disease where P2X7 influences neuroinflammation and neuronal death interacting with proteins such as amyloid-beta.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--western-blot-img29130.jpg "Western blot - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)")
Western blot - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)
This data was developed using Anti-P2X7 antibody [EPR24130-77] ab259942, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
The molecular weight observed is consistent with what has been described in the literature (PMID: 20450227)
The expression profile is consistent with what has been described in the literature (PMID: 29018292)
Low expression or negative control: Mouse skeletal muscle (PMID:15254086, PMID:22415881), which is consistent to IHC expression pattern.
Exposure time: 59 seconds
All lanes: Western blot - Anti-P2X7 antibody [EPR24130-77] (Anti-P2X7 antibody [EPR24130-77] ab259942) at 1/1000 dilution
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Lane 4: Mouse skeletal muscle tissue lysate at 20 µg
SecondaryLanes 1 - 4: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution
Lanes 1 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29821.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)
This data was developed using Anti-P2X7 antibody [EPR24130-77] ab259942, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling P2X7 with Anti-P2X7 antibody [EPR24130-77] ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse cerebrum (PMID: 28716092).The section was incubated with Anti-P2X7 antibody [EPR24130-77] ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4180.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)
This data was developed using Anti-P2X7 antibody [EPR24130-77] ab259942, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling P2X7 with Anti-P2X7 antibody [EPR24130-77] ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining in mouse spleen (PMID: 22415881).The section was incubated with Anti-P2X7 antibody [EPR24130-77] ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99414.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)
This data was developed using Anti-P2X7 antibody [EPR24130-77] ab259942, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling P2X7 with Anti-P2X7 antibody [EPR24130-77] ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining in sinusiod of mouse liver (PMID: 22415881).The section was incubated with Anti-P2X7 antibody [EPR24130-77] ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunoprecipitation - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunoprecipitation-img11655.jpg "Immunoprecipitation - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)")
Immunoprecipitation - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)
This data was developed using Anti-P2X7 antibody [EPR24130-77] ab259942, the same antibody clone in a different buffer formulation.
P2X7 was immunoprecipitated from 0.35 mg Mouse lung tissue lysate 10 ug with Anti-P2X7 antibody [EPR24130-77] ab259942 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-P2X7 antibody [EPR24130-77] ab259942 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse lung tissue lysate 10 ug
Lane 2: Anti-P2X7 antibody [EPR24130-77] ab259942 IP in Mouse lung tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-P2X7 antibody [EPR24130-77] ab259942 in Mouse lung tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds
All lanes: Immunoprecipitation - Anti-P2X7 antibody [EPR24130-77] (Anti-P2X7 antibody [EPR24130-77] ab259942)
Predicted band size: 69 kDa
Observed band size: 75 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133913.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)
This data was developed using Anti-P2X7 antibody [EPR24130-77] ab259942, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling P2X7 with Anti-P2X7 antibody [EPR24130-77] ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Very weak staining in mouse skeletal muscle.The section was incubated with Anti-P2X7 antibody [EPR24130-77] ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709), expandable thumbnail](https://content.abcam.com/products/images/p2x7-antibody-epr24130-77-bsa-and-azide-free-ab279709--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133913.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR24130-77] - BSA and Azide free (ab279709)")
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