Anti-P2X7 antibody [EPR26989-11]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR26989-11] (AB307718)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling P2X7 with ab307718 at 1/1000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum.The section was incubated with ab307718 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR26989-11] (AB307718)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling P2X7 with ab307718 at 1/1000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID : 28716092).The section was incubated with ab307718 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-P2X7 antibody [EPR26989-11] (AB307718)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : 293T (PMID : 33042257). Exposure time : Lane 1 : 3 seconds; Lanes 2-4 : 26 seconds.

All lanes:

Western blot - Anti-P2X7 antibody [EPR26989-11] (ab307718) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SK-MEL-28 (human malignant melanoma cell ) whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Lanes 1 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Observed band size: 75 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-P2X7 antibody [EPR26989-11] (AB307718)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1 : 15 seconds; Lane 2 : 37 seconds; Lanes 3-4 : 26 seconds; Lanes 5-6 : 125 seconds.

All lanes:

Western blot - Anti-P2X7 antibody [EPR26989-11] (ab307718) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse lu tissue lysate at 20 µg

Lane 4:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 5:

Rat cerebellum tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

false

Exposure time: 15s