Skip to main content

Rabbit Recombinant Monoclonal P2X7 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-P2X7 antibody [EPR26989-11] (AB307718), expandable thumbnail
  • Western blot - Anti-P2X7 antibody [EPR26989-11] (AB307718), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR26989-11] (AB307718), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR26989-11] (AB307718), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIPFlow CytICC/IF
Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells (PubMed:21821797, PubMed:23303206).

Alternative names

P2X purinoceptor 7, P2X7, ATP receptor, P2Z receptor, Purinergic receptor, P2RX7

Recommended products

Rabbit Recombinant Monoclonal P2X7 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26989-11
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The P2X7 receptor also known as P2RX7 is a ligand-gated ion channel primarily activated by ATP. It possesses a mass of approximately 68 kDa and is mainly expressed in immune cells like macrophages and microglia. P2X7 functions as a trimer forming a pore in the cell membrane that allows the passage of ions such as calcium (Ca²⁺) and sodium (Na⁺) influencing cell signaling and survival. This receptor is notable for its unique ability to change permeability to larger molecules upon prolonged stimulation with ATP.

Biological function summary

The P2X7 receptor influences immune response and inflammation. It plays a role in the release of pro-inflammatory cytokines like interleukin-1β (IL-1β) therefore participating in the regulation of inflammatory and immune responses. It forms a complex with other purinergic receptors affecting processes such as apoptosis and cell proliferation. The activation of P2X7 can lead to the formation of the inflammasome a component critical for cytokine processing and release.

Pathways

The P2X7 receptor integrates into the NOD-like receptor (NLR) signaling and the purinergic signaling pathways. It interacts with proteins from these pathways including NLRP3 and pannexin-1 which contribute to inflammasome assembly and function. P2X7 also impacts intracellular signaling cascades such as the PI3K/AKT pathway influencing cellular survival and immune responses.

Associated diseases and disorders

Aberrant P2X7 receptor activity has connections to inflammatory and neurodegenerative conditions. In chronic inflammation the enhanced activity of this receptor can exacerbate diseases like rheumatoid arthritis by promoting excessive cytokine release. It is also associated with neurodegenerative disorders like Alzheimer's disease where P2X7 influences neuroinflammation and neuronal death interacting with proteins such as amyloid-beta.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-P2X7 antibody [EPR26989-11] (ab307718), expandable thumbnail

    Western blot - Anti-P2X7 antibody [EPR26989-11] (ab307718)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: 293T (PMID:33042257).
    Exposure time: Lane 1: 3 seconds;
    Lanes 2-4: 26 seconds.

    All lanes: Western blot - Anti-P2X7 antibody [EPR26989-11] (ab307718) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: SK-MEL-28 (human malignant melanoma cell ) whole cell lysate at 20 µg

    Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

    Lanes 1 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

    Observed band size: 75 kDa

    Exposure time: 3s

  • Western blot - Anti-P2X7 antibody [EPR26989-11] (ab307718), expandable thumbnail

    Western blot - Anti-P2X7 antibody [EPR26989-11] (ab307718)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1: 15 seconds;
    Lane 2: 37 seconds;
    Lanes 3-4: 26 seconds;
    Lanes 5-6: 125 seconds.

    All lanes: Western blot - Anti-P2X7 antibody [EPR26989-11] (ab307718) at 1/1000 dilution

    Lane 1: Mouse cerebellum tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Mouse lu tissue lysate at 20 µg

    Lane 4: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 5: Rat cerebellum tissue lysate at 20 µg

    Lane 6: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75 kDa

    Exposure time: 15s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR26989-11] (ab307718), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR26989-11] (ab307718)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling P2X7 with ab307718 at 1/1000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum.The section was incubated with ab307718 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR26989-11] (ab307718), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody [EPR26989-11] (ab307718)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling P2X7 with ab307718 at 1/1000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID: 28716092).The section was incubated with ab307718 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com