AB233760

Anti-P2Y12 antibody [1C2A9]

Name: Anti-P2Y12 antibody [1C2A9] (ab233760) | Abcam
Brand: Abcam Limited
SKU: AB233760

(1 Review)

(2 Publications)

Mouse Monoclonal P2Y12 antibody. Suitable for Flow Cyt, WB and reacts with Human, Rat, Transfected cell lysate - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P2RY12 aa 1-50.

View Alternative Names

HORK3, P2RY12, P2Y purinoceptor 12, P2Y12, ADP-glucose receptor, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, SP1999, ADPG-R, P2Y(ADP)

4 Images

Flow Cytometry - Anti-P2Y12 antibody [1C2A9] (AB233760)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-P2Y12 antibody [1C2A9] (AB233760)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling P2Y12 with ab233760 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-P2Y12 antibody [1C2A9] (AB233760)

All lanes:

Western blot - Anti-P2Y12 antibody [1C2A9] (ab233760) at 1/500 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma cell line) cell lysate

Lane 2:

C6 (rat glioma cell line) cell lysate

Predicted band size: 39 kDa

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Supplier Data

Western blot - Anti-P2Y12 antibody [1C2A9] (AB233760)

Expected MW is 9.4 kDa.

All lanes:

Western blot - Anti-P2Y12 antibody [1C2A9] (ab233760) at 1/500 dilution

All lanes:

Recombinant human P2Y12 protein

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-P2Y12 antibody [1C2A9] (AB233760)

All lanes:

Western blot - Anti-P2Y12 antibody [1C2A9] (ab233760) at 1/500 dilution

Lane 1:

Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

Human P2Y12-hIgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 39 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1C2A9

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

WB, Flow Cyt

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

Recombinant Fragment Protein within Human P2RY12 aa 50-100. Database link Q9H244

The exact immunogen used to generate this antibody is proprietary information.

Recombinant Fragment Protein within Human P2RY12 aa 150-200. Database link Q9H244

The exact immunogen used to generate this antibody is proprietary information.

Recombinant Fragment Protein within Human P2RY12 aa 250-300. Database link Q9H244

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P2Y12 also referred to as the P2Y12 receptor is a G-protein coupled receptor with an approximate mass of 39.6 kDa. It plays a distinct role in platelet aggregation through ADP binding which activates intracellular signaling pathways that result in platelet activation. One finds P2Y12 expressed mainly in platelets and the brain. It is an important target of various antiplatelet drugs which highlights its critical role in physiological frameworks involving thrombosis.

Biological function summary

The P2Y12 receptor is an important component of the platelet activation pathway. When activated it contributes to the amplification of platelet responses to injury. P2Y12 does not function in isolation; it forms part of larger multi-protein complexes essential for thrombus formation. This aggregation process requires synchronized activation of several platelet receptors emphasizing the P2Y12 receptor's critical role in maintaining hemostasis through cellular signaling.

Pathways

The P2Y12 receptor is integral to the thrombin and ADP signaling pathways functioning in tandem with other proteins such as the P2Y1 and CXCR4 receptors to execute cellular responses. These pathways facilitate platelet shape change secretion and aggregation therefore solidifying their place within the broader scope of hemostatic responses. Coordination with such protein partners ensures efficient propagation of signals necessary for rapid platelet activation and clot formation.

P2Y12 is closely linked to cardiovascular diseases particularly thrombosis and myocardial infarction. Its involvement in excessive platelet aggregation makes it a significant target for therapeutic agents like the P2Y12 receptor blockers. The receptor also interacts with proteins like glycoprotein IIb/IIIa in the clotting process providing potential pathways for therapeutic intervention. Dysregulation of P2Y12 function presents a risk for unwarranted thrombotic events highlighting the importance of P2Y12 antagonists such as ticagrelor in clinical settings to manage such conditions effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation.

See full target information P2RY12

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC musculoskeletal disorders 25:1081 PubMed39736635

2024

The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Wang,Zhengwei Duan,Zihua Li,Dong Yang,Hengli Lu,Yiwei Zhang,Yuesong Fu,Yonghao Guan,Guodong Li,Feng Qian,Tianyang Xu

Immunity, inflammation and disease 10:e697 PubMed36169256

2022

Inhibiting P2Y12 receptor relieves LPS-induced inflammation and endothelial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuxia Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P2Y12 antibody [1C2A9]

Flow Cytometry - Anti-P2Y12 antibody [1C2A9] (AB233760)

Western blot - Anti-P2Y12 antibody [1C2A9] (AB233760)

Western blot - Anti-P2Y12 antibody [1C2A9] (AB233760)

Western blot - Anti-P2Y12 antibody [1C2A9] (AB233760)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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