AB184411

Anti-P2Y12 antibody [EPR18611]

Name: Anti-P2Y12 antibody [EPR18611] (ab184411) | Abcam
Brand: Abcam Limited
SKU: AB184411
Price: 320 USD
Availability: InStock
Rating: 1 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(14 Publications)

Rabbit Recombinant Monoclonal P2Y12 antibody. Suitable for IP, WB and reacts with Rat, Mouse samples. Cited in 14 publications.

View Alternative Names

P2Y purinoceptor 12, P2Y12, P2ry12

3 Images

Supplier Data

Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] (AB184411)

P2Y12 was immunoprecipitated from 1mg of Rat brain whole cell lysate with ab184411 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab184411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Rat brain whole cell lysate 10μg (Input).
Lane 2 : ab184411 IP in Rat brain whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184411 in Rat brain whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] (ab184411)

Predicted band size: 39 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-P2Y12 antibody [EPR18611] (AB184411)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 39 kDa

Observed band size: 50 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-P2Y12 antibody [EPR18611] (AB184411)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 39 kDa

Observed band size: 50 kDa

false

Exposure time: 1s

Carrier free

Anti-P2Y12 antibody [EPR18611] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18611

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P2Y12 also referred to as the P2Y12 receptor is a G-protein coupled receptor with an approximate mass of 39.6 kDa. It plays a distinct role in platelet aggregation through ADP binding which activates intracellular signaling pathways that result in platelet activation. One finds P2Y12 expressed mainly in platelets and the brain. It is an important target of various antiplatelet drugs which highlights its critical role in physiological frameworks involving thrombosis.

Biological function summary

The P2Y12 receptor is an important component of the platelet activation pathway. When activated it contributes to the amplification of platelet responses to injury. P2Y12 does not function in isolation; it forms part of larger multi-protein complexes essential for thrombus formation. This aggregation process requires synchronized activation of several platelet receptors emphasizing the P2Y12 receptor's critical role in maintaining hemostasis through cellular signaling.

Pathways

The P2Y12 receptor is integral to the thrombin and ADP signaling pathways functioning in tandem with other proteins such as the P2Y1 and CXCR4 receptors to execute cellular responses. These pathways facilitate platelet shape change secretion and aggregation therefore solidifying their place within the broader scope of hemostatic responses. Coordination with such protein partners ensures efficient propagation of signals necessary for rapid platelet activation and clot formation.

P2Y12 is closely linked to cardiovascular diseases particularly thrombosis and myocardial infarction. Its involvement in excessive platelet aggregation makes it a significant target for therapeutic agents like the P2Y12 receptor blockers. The receptor also interacts with proteins like glycoprotein IIb/IIIa in the clotting process providing potential pathways for therapeutic intervention. Dysregulation of P2Y12 function presents a risk for unwarranted thrombotic events highlighting the importance of P2Y12 antagonists such as ticagrelor in clinical settings to manage such conditions effectively.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Required for normal platelet aggregation and blood coagulation.

See full target information P2ry12

style

left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Annals of neurology 96:390-404 PubMed38747498

2024

γ-Secretase Modulator BPN15606 Reduced Aβ42 and Aβ40 and Countered Alzheimer-Related Pathologies in a Mouse Model of Down Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Qiao Chen,Ann Becker,Ricardo Albay,Phuong D Nguyen,Dmitry Karachentsev,Amanda J Roberts,Kevin D Rynearson,Rudolph E Tanzi,William C Mobley

Communications biology 6:1239 PubMed38066234

2023

Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojun Liu,Dongyu Li,Tingting Yu,Jingtan Zhu,Oxana Semyachkina-Glushkovskaya,Dan Zhu

Journal of neuroinflammation 20:193 PubMed37612735

2023

GSDMD knockdown attenuates phagocytic activity of microglia and exacerbates seizure susceptibility in TLE mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxia Yang,Qingqing Cao,Yi Guo,Jingchuan He,Demei Xu,Aolei Lin

Frontiers in pharmacology 13:873090 PubMed35529431

2022

Naringin Relieves Diabetic Cardiac Autonomic Neuropathy Mediated by P2Y Receptor in Superior Cervical Ganglion.

Applications

Unspecified application

Species

Unspecified reactive species

Gan Tang,Lingzhi Pi,Hongmin Guo,Zihui Hu,Congfa Zhou,Qixing Hu,Hao Peng,Zehao Xiao,Zhihua Zhang,Miaomiao Wang,Taotao Peng,Jiaqi Huang,Shangdong Liang,Guilin Li

British journal of pharmacology 179:46-64 PubMed34415054

2021

Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ping Jiang,Jin-Zi Ji,Peng-Xin Ge,Ting Zhu,Qiong-Yu Mi,Ting Tai,Yi-Fei Li,Hong-Guang Xie

Neural regeneration research 17:203-209 PubMed34100457

2021

extract promotes M2 polarization and reduces oligomeric amyloid-β-induced inflammatory reactions in microglial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Qing Sun,Jin-Feng Liu,Wei Luo,Ching-Hin Wong,Kwok-Fai So,Yong Hu,Kin Chiu

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:413-420 PubMed33908199

2021

P2Y12 inhibition in macrophages reduces ventricular arrhythmias in rats after myocardial ischemia-reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Wang,Na Li,Fei Wang,Lianqun Cui

Frontiers in aging neuroscience 13:654509 PubMed33867971

2021

Western Diet Induces Impairment of Liver-Brain Axis Accelerating Neuroinflammation and Amyloid Pathology in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Angelika Wiȩckowska-Gacek,Anna Mietelska-Porowska,Dominik Chutorański,Małgorzata Wydrych,Jan Długosz,Urszula Wojda

EMBO molecular medicine 11: PubMed31122931

2019

Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Julia K Götzl,Matthias Brendel,Georg Werner,Samira Parhizkar,Laura Sebastian Monasor,Gernot Kleinberger,Alessio-Vittorio Colombo,Maximilian Deussing,Matias Wagner,Juliane Winkelmann,Janine Diehl-Schmid,Johannes Levin,Katrin Fellerer,Anika Reifschneider,Sebastian Bultmann,Peter Bartenstein,Axel Rominger,Sabina Tahirovic,Scott T Smith,Charlotte Madore,Oleg Butovsky,Anja Capell,Christian Haass

Journal of ethnopharmacology 235:100-110 PubMed30710735

2019

Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Min Xiao,Chenyu Qian,Xi Luo,Mengbi Yang,Yufeng Zhang,Cheyuen Wu,Chungtong Mok,Puiwai Lee,Zhong Zuo

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P2Y12 antibody [EPR18611]

Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] (AB184411)

Western blot - Anti-P2Y12 antibody [EPR18611] (AB184411)

Western blot - Anti-P2Y12 antibody [EPR18611] (AB184411)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise