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AB240285

Anti-P2Y12 antibody [EPR18611] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal P2Y12 antibody. Carrier free. Suitable for IP, WB and reacts with Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

P2Y purinoceptor 12, P2Y12, P2ry12

1 Images
Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] - BSA and Azide free (AB240285)
  • IP

Supplier Data

Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] - BSA and Azide free (AB240285)

P2Y12 was immunoprecipitated from 1mg of Rat brain whole cell lysate with ab184411 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab184411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : Rat brain whole cell lysate 10μg (Input).
Lane 2 : ab184411 IP in Rat brain whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184411 in Rat brain whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184411).

All lanes:

Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] (<a href='/en-us/products/primary-antibodies/p2y12-antibody-epr18611-ab184411'>ab184411</a>)

Predicted band size: 39 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18611

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab240285 is the carrier-free version of ab184411.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P2Y12 also referred to as the P2Y12 receptor is a G-protein coupled receptor with an approximate mass of 39.6 kDa. It plays a distinct role in platelet aggregation through ADP binding which activates intracellular signaling pathways that result in platelet activation. One finds P2Y12 expressed mainly in platelets and the brain. It is an important target of various antiplatelet drugs which highlights its critical role in physiological frameworks involving thrombosis.
Biological function summary

The P2Y12 receptor is an important component of the platelet activation pathway. When activated it contributes to the amplification of platelet responses to injury. P2Y12 does not function in isolation; it forms part of larger multi-protein complexes essential for thrombus formation. This aggregation process requires synchronized activation of several platelet receptors emphasizing the P2Y12 receptor's critical role in maintaining hemostasis through cellular signaling.

Pathways

The P2Y12 receptor is integral to the thrombin and ADP signaling pathways functioning in tandem with other proteins such as the P2Y1 and CXCR4 receptors to execute cellular responses. These pathways facilitate platelet shape change secretion and aggregation therefore solidifying their place within the broader scope of hemostatic responses. Coordination with such protein partners ensures efficient propagation of signals necessary for rapid platelet activation and clot formation.

P2Y12 is closely linked to cardiovascular diseases particularly thrombosis and myocardial infarction. Its involvement in excessive platelet aggregation makes it a significant target for therapeutic agents like the P2Y12 receptor blockers. The receptor also interacts with proteins like glycoprotein IIb/IIIa in the clotting process providing potential pathways for therapeutic intervention. Dysregulation of P2Y12 function presents a risk for unwarranted thrombotic events highlighting the importance of P2Y12 antagonists such as ticagrelor in clinical settings to manage such conditions effectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Required for normal platelet aggregation and blood coagulation.
See full target information P2ry12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of histochemistry : EJH 65: PubMed34587716

2021

Long noncoding RNA Meg3 mediates ferroptosis induced by oxygen and glucose deprivation combined with hyperglycemia in rat brain microvascular endothelial cells, through modulating the p53/GPX4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Yan Huang,Pingping Xia,Fan Zhang,Longyan Li,E Wang,Qulian Guo,Zhi Ye
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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