Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386)

Fluorescence multiplex immunohistochemical analysis of the Human cerebrum (Formalin/PFA-fixed paraffin-embedded sections).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Opal Polymer HRP Ms + Rb was used as a secondary antibody.DAPI (blue) was used as a nuclear counter stain.

Panel A : Merged staining of anti-GFAP (gray; Opal™690), anti-P2Y12 (green; Opal™520) and anti-MAP2 (red; Opal™570) on human cerebrum. Panel B : Anti-MAP2 stained cell body and dendrites of neurons. Panel C : Anti-P2Y12 stained on microglial cells. Panel D : Anti-GFAP stained on astrocytes.

The section was incubated in three rounds of staining : in the order of ab68428, ab254347, and ab254263 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386)

This data was developed using ab254347, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling P2Y12 with ab254347 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human glioma (PMID : 30832693). The section was incubated with ab254347 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386)

Fluorescence multiplex immunohistochemical analysis of the Human cerebellum (Formalin/PFA-fixed paraffin-embedded sections).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Opal Polymer HRP Ms + Rb was used as a secondary antibody.DAPI (blue) was used as a nuclear counter stain.

Panel A : Merged staining of anti-GFAP (gray; Opal™690), anti-P2Y12 (green; Opal™520) and anti-MAP2 (red; Opal™570) on human cerebellum. Panel B : Anti-MAP2 stained cell body and dendrites of neurons. Panel C : Anti-P2Y12 stained on microglial cells. Panel D : Anti-GFAP stained on astrocytes.

The section was incubated in three rounds of staining : in the order of ab68428, ab254347, and ab254263 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386)

Fluorescence multiplex immunohistochemical analysis of the human cerebellum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-GFAP (ab68428, gray; Opal™690), anti-Myelin PLP (ab254363, green; Opal™520) and anti-P2Y12 (ab254347 red; Opal™570) on human cerebellum. Panel B : anti-P2Y12 stained on microglial cells. Panel C : anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D : anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab68428 (1/50 dilution), ab254363 (1/2000 dilution), and ab254347 (1/1000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab254347, the same antibody clone in a different buffer formulation.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386)

This data was developed using ab254347, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling P2Y12 with ab254347 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on microglia (PMID : 30832693). The section was incubated with ab254347 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386)

Fluorescence multiplex immunohistochemical analysis of the human cerebrum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-GFAP (ab68428, gray; Opal™690), anti-Myelin PLP (ab254363, green; Opal™520) and anti-P2Y12 (ab254347, red; Opal™570) on human cerebrum. Panel B : anti-P2Y12 stained on microglial cells. Panel C : anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D : anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab68428 (1/50 dilution), ab254363 (1/2000 dilution), and ab254347 (1/1000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab254347, the same antibody clone in a different buffer formulation.

• Dot

Unknown

Dot Blot - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386)

This data was developed using ab254347, the same antibody clone in a different buffer formulation.

Dot blot analysis of P2Y12 using ab254347 at 1/1000 dilution (0.569 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1 : Human P2Y12 immunogen peptide (aa 162-181)

Lane 2 : Human P2Y12 non-immunogen peptide (aa 323-340)

Lane 3 : Human P2Y12 non-immunogen peptide (aa 308-328)

Lane 4 : BSA

Exposure time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST.