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Rabbit Recombinant Monoclonal P2Y12 antibody. Carrier free. Suitable for mIHC, Dot, IHC-P and reacts with Human samples.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386), expandable thumbnail
  • Dot Blot - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCICC/IFIPFlow CytDotWBIHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 products for Alternative Version

Target data

Function

Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation.

Alternative names

P2Y purinoceptor 12, P2Y12, ADP-glucose receptor, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, SP1999, ADPG-R, P2Y(ADP), HORK3, P2RY12

Recommended products

Rabbit Recombinant Monoclonal P2Y12 antibody. Carrier free. Suitable for mIHC, Dot, IHC-P and reacts with Human samples.

Alternative names

P2Y purinoceptor 12, P2Y12, ADP-glucose receptor, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, SP1999, ADPG-R, P2Y(ADP), HORK3, P2RY12

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR23511-72

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab274386 is the carrier-free version of Anti-P2Y12 antibody [EPR23511-72] ab254347.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

P2Y12 also referred to as the P2Y12 receptor is a G-protein coupled receptor with an approximate mass of 39.6 kDa. It plays a distinct role in platelet aggregation through ADP binding which activates intracellular signaling pathways that result in platelet activation. One finds P2Y12 expressed mainly in platelets and the brain. It is an important target of various antiplatelet drugs which highlights its critical role in physiological frameworks involving thrombosis.

Biological function summary

The P2Y12 receptor is an important component of the platelet activation pathway. When activated it contributes to the amplification of platelet responses to injury. P2Y12 does not function in isolation; it forms part of larger multi-protein complexes essential for thrombus formation. This aggregation process requires synchronized activation of several platelet receptors emphasizing the P2Y12 receptor's critical role in maintaining hemostasis through cellular signaling.

Pathways

The P2Y12 receptor is integral to the thrombin and ADP signaling pathways functioning in tandem with other proteins such as the P2Y1 and CXCR4 receptors to execute cellular responses. These pathways facilitate platelet shape change secretion and aggregation therefore solidifying their place within the broader scope of hemostatic responses. Coordination with such protein partners ensures efficient propagation of signals necessary for rapid platelet activation and clot formation.

Associated diseases and disorders

P2Y12 is closely linked to cardiovascular diseases particularly thrombosis and myocardial infarction. Its involvement in excessive platelet aggregation makes it a significant target for therapeutic agents like the P2Y12 receptor blockers. The receptor also interacts with proteins like glycoprotein IIb/IIIa in the clotting process providing potential pathways for therapeutic intervention. Dysregulation of P2Y12 function presents a risk for unwarranted thrombotic events highlighting the importance of P2Y12 antagonists such as ticagrelor in clinical settings to manage such conditions effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    This data was developed using Anti-P2Y12 antibody [EPR23511-72] ab254347, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR23511-72] ab254347 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in human glioma (PMID: 30832693). The section was incubated with Anti-P2Y12 antibody [EPR23511-72] ab254347 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Dot Blot - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386), expandable thumbnail

    Dot Blot - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    This data was developed using Anti-P2Y12 antibody [EPR23511-72] ab254347, the same antibody clone in a different buffer formulation.

    Dot blot analysis of P2Y12 using Anti-P2Y12 antibody [EPR23511-72] ab254347 at 1/1000 dilution (0.569 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.

    Lane 1: Human P2Y12 immunogen peptide (aa 162-181)

    Lane 2: Human P2Y12 non-immunogen peptide (aa 323-340)

    Lane 3: Human P2Y12 non-immunogen peptide (aa 308-328)

    Lane 4: BSA

    Exposure time: 3 minutes

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    This data was developed using Anti-P2Y12 antibody [EPR23511-72] ab254347, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR23511-72] ab254347 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on microglia (PMID: 30832693). The section was incubated with Anti-P2Y12 antibody [EPR23511-72] ab254347 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    Fluorescence multiplex immunohistochemical analysis of the Human cerebrum (Formalin/PFA-fixed paraffin-embedded sections).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    Opal Polymer HRP Ms + Rb was used as a secondary antibody.DAPI (blue) was used as a nuclear counter stain.

    Panel A: Merged staining of anti-GFAP (gray; Opal™690), anti-P2Y12 (green; Opal™520) and anti-MAP2 (red; Opal™570) on human cerebrum.
    Panel B: Anti-MAP2 stained cell body and dendrites of neurons.
    Panel C: Anti-P2Y12 stained on microglial cells.
    Panel D: Anti-GFAP stained on astrocytes.

    The section was incubated in three rounds of staining: in the order of Anti-GFAP antibody [EPR1034Y] ab68428, Anti-P2Y12 antibody [EPR23511-72] ab254347, and Anti-MAP2 antibody [EPR22641-106] ab254263 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386), expandable thumbnail

    Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    Fluorescence multiplex immunohistochemical analysis of the human cerebellum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-GFAP (Anti-GFAP antibody [EPR1034Y] ab68428, gray; Opal™690), anti-Myelin PLP (Anti-Myelin PLP antibody [EPR23504-106] ab254363, green; Opal™520) and anti-P2Y12 (Anti-P2Y12 antibody [EPR23511-72] ab254347 red; Opal™570) on human cerebellum. Panel B: anti-P2Y12 stained on microglial cells. Panel C: anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D: anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-GFAP antibody [EPR1034Y] ab68428 (1/50 dilution), Anti-Myelin PLP antibody [EPR23504-106] ab254363 (1/2000 dilution), and Anti-P2Y12 antibody [EPR23511-72] ab254347 (1/1000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.



    This data was developed using Anti-P2Y12 antibody [EPR23511-72] ab254347, the same antibody clone in a different buffer formulation.

  • Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386), expandable thumbnail

    Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    Fluorescence multiplex immunohistochemical analysis of the human cerebrum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-GFAP (Anti-GFAP antibody [EPR1034Y] ab68428, gray; Opal™690), anti-Myelin PLP (Anti-Myelin PLP antibody [EPR23504-106] ab254363, green; Opal™520) and anti-P2Y12 (Anti-P2Y12 antibody [EPR23511-72] ab254347, red; Opal™570) on human cerebrum. Panel B: anti-P2Y12 stained on microglial cells. Panel C: anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D: anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-GFAP antibody [EPR1034Y] ab68428 (1/50 dilution), Anti-Myelin PLP antibody [EPR23504-106] ab254363 (1/2000 dilution), and Anti-P2Y12 antibody [EPR23511-72] ab254347 (1/1000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.



    This data was developed using Anti-P2Y12 antibody [EPR23511-72] ab254347, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    Fluorescence multiplex immunohistochemical analysis of the Human cerebellum (Formalin/PFA-fixed paraffin-embedded sections).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Opal Polymer HRP Ms + Rb was used as a secondary antibody.DAPI (blue) was used as a nuclear counter stain.

    Panel A: Merged staining of anti-GFAP (gray; Opal™690), anti-P2Y12 (green; Opal™520) and anti-MAP2 (red; Opal™570) on human cerebellum.
    Panel B: Anti-MAP2 stained cell body and dendrites of neurons.
    Panel C: Anti-P2Y12 stained on microglial cells.
    Panel D: Anti-GFAP stained on astrocytes.

    The section was incubated in three rounds of staining: in the order of Anti-GFAP antibody [EPR1034Y] ab68428, Anti-P2Y12 antibody [EPR23511-72] ab254347, and Anti-MAP2 antibody [EPR22641-106] ab254263 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BONDBOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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Product protocols

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