Anti-P2Y12 antibody [EPR26298-93]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling P2Y12 with ab300140 at 1/40000 (0.013 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on human liver. The section was incubated with ab300140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

Positive staining on microglial cells in human cerebrum (PMID : 30196821). The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Negative control : no staining on human liver. The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 (0.013 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on microglial cells in human cerebrum (PMID : 30196821). The section was incubated with ab300140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistological analysis of 4% PFA fixed and 0.2% Triton X-100 parmeabilized frozen mouse cerebrum (fresh) tissue labeling P2Y12 with ab300140 at 1/500 dilution, followed by (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution. DAPI was used as nuclear counterstain.

Positive staining on mouse cerebrum.

Secondary antibody only control used PBS instead of primary antibody, followed by secondary (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Positive staining on microglial cells in rat cerebrum (PMID : 30196821). The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistological analysis of 4% PFA fixed and 0.2% Triton X-100 parmeabilized frozen rat cerebrum tissue labeling P2Y12 with ab300140 at 1/500 dilution, followed by (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution. DAPI was used as nuclear counterstain.

Positive staining on rat cerebrum.

Secondary antibody only control used PBS instead of primary antibody, followed by secondary (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 (0.013 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on microglial cells in mouse cerebrum (PMID : 30196821). The section was incubated with ab300140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 (0.013 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on microglial cells in rat cerebrum (PMID : 30196821). The section was incubated with ab300140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling P2Y12 with ab300140 at 1/500 (1.052 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling P2Y12 with ab300140 at 1/500 (1.052 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse midbrain tissue staining THP2 with ab288067 at a 1/2000 (0.315 ug/ml) dilution, P2Y12 with ab300140 at 1/40000 (0.013 ug/ml) dilution and GFAP with ab218309 at 1/1000 ( 1.325 ug/ml) dilution.

Panel A : merged staining of anti-THP2 (green; Opal™520), anti-P2Y12 (grey; Opal™570) and anti-GFAP (magenta; Opal™690) on mouse midbrain.
Panel B : anti-THP2 staining the serotonergic neurons in mouse midbrain.
Panel C : anti-P2Y12 staining microglia in mouse midbrain.
Panel D : anti-GFAP staining astrocytes in mouse midbrain.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab2888067, ab300140 and ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse spinal cord.

Panel B : anti-GPR17 staining oligodendrocytes in mouse spinal cord.

Panel C : anti-P2RY12 staining microglia in mouse spinal cord.

Panel D : anti-GFAP staining astrocytes in mouse spinal cord.

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse cerebellum.

Panel B : anti-GPR17 staining oligodendrocytes in mouse cerebellum.

Panel C : anti-P2RY12 staining microglia in mouse cerebellum.

Panel D : anti-GFAP staining astrocytes in mouse cerebellum.

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on rat spinal cord.

Panel B : anti-GPR17 staining oligodendrocytes in rat spinal cord.

Panel C : anti-P2RY12 staining microglia in rat spinal cord.

Panel D : anti-GFAP staining astrocytes in rat spinal cord.

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse spinal cord.
Panel B : anti-GPR17 staining oligodendrocytes in mouse spinal cord.
Panel C : anti-P2RY12 staining microglia in mouse spinal cord.
Panel D : anti-FMR1 staining neurons in mouse spinal cord.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse hippocampus.

Panel B : anti-GPR17 staining oligodendrocytes in mouse hippocampus.

Panel C : anti-P2RY12 staining microglia in mouse hippocampus.

Panel D : anti-GFAP staining astrocytes in mouse hippocampus.

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse hippocampus.

Panel B : anti-GPR17 staining oligodendrocytes in mouse hippocampus.

Panel C : anti-P2RY12 staining microglia in mouse hippocampus.

Panel D : anti-FMR1 staining neurons in mouse hippocampus.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstaining with DAPI.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on rat cerebrum.

Panel B : anti-GPR17 staining oligodendrocytes in rat cerebrum.

Panel C : anti-P2RY12 staining microglia in rat cerebrum.

Panel D : anti-FMR1 staining neurons in rat cerebrum.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstaining with DAPI.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse cerebellum.

Panel B : anti-GPR17 staining oligodendrocytes in mouse cerebellum.

Panel C : anti-P2RY12 staining microglia in mouse cerebellum.

Panel D : anti-FMR1 staining neurons and Purkinje cells in mouse cerebellum.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat hippocampus tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on rat hippocampus.

Panel B : anti-GPR17 staining oligodendrocytes in rat hippocampus.

Panel C : anti-P2RY12 staining microglia in rat hippocampus.

Panel D : anti-FMR1 staining neurons in rat hippocampus.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse cerebrum.

Panel B : anti-GPR17 staining oligodendrocytes in mouse cerebrum.

Panel C : anti-P2RY12 staining microglia in mouse cerebrum.

Panel D : anti-GFAP staining astrocytes in mouse cerebrum.

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/140000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on rat cerebrum.

Panel B : anti-GPR17 staining oligodendrocytes in rat cerebrum.

Panel C : anti-P2RY12 staining microglia in rat cerebrum.

Panel D : anti-GFAP staining astrocytes in rat cerebrum.

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/140000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue staining Olig1 with ab325492 at a 1 : 500 (1.036 µg/ml) dilution, ab68428 anti-GFAP used at 1 : 500 (0.104 µg/ml) dilution and ab300140 anti-P2Y12 used at a 1 : 40000 (0.013 µg/ml) dilution.

Panel A : anti-Olig1 (green; Opal™520), anti-GFAP (magenta; Opal™690) and anti-P2Y12 (yellow; Opal™570) on mouse cerebellum.
Panel B : anti-Olig1 staining oligodendrocytes in mouse cerebellum.
Panel C : anti-GFAP staining astrocytes in mouse cerebellum.
Panel D : anti-P2Y12 staining microglia in mouse cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325492, ab68428 and ab300140 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse cerebrum.

Panel B : anti-GPR17 staining oligodendrocytes in mouse cerebrum.

Panel C : anti-P2RY12 staining microglia in mouse cerebrum.

Panel D : anti-FMR1 staining neurons in mouse cerebrum.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue staining Olig1 with ab325492 at a 1 : 500 (1.036 µg/ml) dilution, ab68428 anti-GFAP used at 1 : 500 (0.104 µg/ml) dilution and ab300140 anti-P2Y12 used at a 1 : 40000 (0.013 µg/ml) dilution.

Panel A : anti-Olig1 (green; Opal™520), anti-GFAP (magenta; Opal™690) and anti-P2Y12 (yellow; Opal™570) on mouse cerebrum.
Panel B : anti-Olig1 staining oligodendrocytes in mouse cerebrum.
Panel C : anti-GFAP staining astrocytes in mouse cerebrum.
Panel D : anti-P2Y12 staining microglia in mouse cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325492, ab68428 and ab300140 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat cerebellum tissue staining Olig1 with ab325492 at a 1 : 500 (1.036 µg/ml) dilution, ab68428 anti-GFAP used at 1 : 500 (0.104 µg/ml) dilution and ab300140 anti-P2Y12 used at a 1 : 40000 (0.013 µg/ml) dilution.

Panel A : anti-Olig1 (green; Opal™520), anti-GFAP (magenta; Opal™690) and anti-P2Y12 (yellow; Opal™570) on rat cerebellum.
Panel B : anti-Olig1 staining oligodendrocytes in rat cerebellum.
Panel C : anti-GFAP staining astrocytes in rat cerebellum.
Panel D : anti-P2Y12 staining microglia in rat cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325492, ab68428 and ab300140 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] (AB300140)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Positive staining on microglial cells in mouse cerebrum (PMID : 30196821). The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).