Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free

25 product images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

  Positive staining on microglial cells in human cerebrum (PMID: 30196821). The section was incubated with Anti-P2Y12 antibody [EPR26298-93] ab300140 for 30 mins at room temperature.
  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  

  Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.

  Heat mediated antigen retrieval was perormed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Positive staining on microglial cells in rat cerebrum (PMID: 30196821). The section was incubated with Anti-P2Y12 antibody [EPR26298-93] ab300140 for 30 mins at room temperature.
  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  

  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Positive staining on microglial cells in mouse cerebrum (PMID: 30196821). The section was incubated with Anti-P2Y12 antibody [EPR26298-93] ab300140 for 30 mins at room temperature.
  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  

  Immunohistochemical analysis of paraffin-embedded human liver tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin.

  Heat mediated antigen retrieval was performedwith Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Negative control: no staining on human liver. The section was incubated with Anti-P2Y12 antibody [EPR26298-93] ab300140 for 30 mins at room temperature.
  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

• 

  Immunohistochemistry (Frozen sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  

  Immunohistological analysis of 4% PFA fixed and 0.2% Triton X-100 parmeabilized frozen rat cerebrum (fresh) tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/500 dilution, followed by (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution. DAPI was used as nuclear counterstain.

  Positive staining on rat cerebrum.

  Secondary antibody only control used PBS instead of primary antibody, followed by secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  

  Immunohistological analysis of 4% PFA fixed and 0.2% Triton X-100 parmeabilized frozen mouse cerebrum (fresh) tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/500 dilution, followed by (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution. DAPI was used as nuclear counterstain.

  Positive staining on mouse cerebrum.

  Secondary antibody only control used PBS instead of primary antibody, followed by secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/500 (1.052 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue sections.

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse cerebellum.

  Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebellum.

  Panel C: anti-P2RY12 staining microglia in mouse cerebellum.

  Panel D: anti-FMR1 staining neurons and Purkinje cells in mouse cerebellum.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000 dilution and Anti-FMRP antibody [EPR23852-90] ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue sections.
  

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse cerebrum.

  Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebrum.

  Panel C: anti-P2RY12 staining microglia in mouse cerebrum.

  Panel D: anti-GFAP staining astrocytes in mouse cerebrum.

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/140000 dilution, and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue sections.
  

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse cerebrum.

  Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebrum.

  Panel C: anti-P2RY12 staining microglia in mouse cerebrum.

  Panel D: anti-FMR1 staining neurons in mouse cerebrum.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000 dilution, and Anti-FMRP antibody [EPR23852-90] ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue sections.
  

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on rat cerebrum.

  Panel B: anti-GPR17 staining oligodendrocytes in rat cerebrum.

  Panel C: anti-P2RY12 staining microglia in rat cerebrum.

  Panel D: anti-GFAP staining astrocytes in rat cerebrum.

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/140000 dilution, and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue sections.
  

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse hippocampus.

  Panel B: anti-GPR17 staining oligodendrocytes in mouse hippocampus.

  Panel C: anti-P2RY12 staining microglia in mouse hippocampus.

  Panel D: anti-FMR1 staining neurons in mouse hippocampus.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000 dilution, and Anti-FMRP antibody [EPR23852-90] ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstaining with DAPI.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue sections.

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on rat cerebrum.

  Panel B: anti-GPR17 staining oligodendrocytes in rat cerebrum.

  Panel C: anti-P2RY12 staining microglia in rat cerebrum.

  Panel D: anti-FMR1 staining neurons in rat cerebrum.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000 dilution, and Anti-FMRP antibody [EPR23852-90] ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstaining with DAPI.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat hippocampus tissue sections.

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on rat hippocampus.

  Panel B: anti-GPR17 staining oligodendrocytes in rat hippocampus.

  Panel C: anti-P2RY12 staining microglia in rat hippocampus.

  Panel D: anti-FMR1 staining neurons in rat hippocampus.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000 dilution, and Anti-FMRP antibody [EPR23852-90] ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue sections.

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse cerebellum.

  Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebellum.

  Panel C: anti-P2RY12 staining microglia in mouse cerebellum.

  Panel D: anti-GFAP staining astrocytes in mouse cerebellum.

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000 dilution, and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Nuclear counterstaining with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue sections.

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse hippocampus.

  Panel B: anti-GPR17 staining oligodendrocytes in mouse hippocampus.

  Panel C: anti-P2RY12 staining microglia in mouse hippocampus.

  Panel D: anti-GFAP staining astrocytes in mouse hippocampus.

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000, and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Nuclear counterstaining with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue sections.

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse spinal cord.

  Panel B: anti-GPR17 staining oligodendrocytes in mouse spinal cord.

  Panel C: anti-P2RY12 staining microglia in mouse spinal cord.

  Panel D: anti-GFAP staining astrocytes in mouse spinal cord.

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000 dilution, and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Nuclear counterstaining with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140 , the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue sections.

  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on rat spinal cord.

  Panel B: anti-GPR17 staining oligodendrocytes in rat spinal cord.

  Panel C: anti-P2RY12 staining microglia in rat spinal cord.

  Panel D: anti-GFAP staining astrocytes in rat spinal cord.

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000 dilution, and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Nuclear counterstaining with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140 , the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue.

  
  Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse spinal cord.
  
  Panel B: anti-GPR17 staining oligodendrocytes in mouse spinal cord.
  
  Panel C: anti-P2RY12 staining microglia in mouse spinal cord.
  
  Panel D: anti-FMR1 staining neurons in mouse spinal cord.
  
  Nuclear DNA was labeled with DAPI (shown in blue).
  

  The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, Anti-P2Y12 antibody [EPR26298-93] ab300140 at a 1/40000 dilution, and Anti-FMRP antibody [EPR23852-90] ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using ab300141, the same antibody clone in a different buffer formulation.

• 

  Multiplex immunohistochemistry - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse midbrain tissue staining THP2 with Anti-TPH2 antibody [EPR25100-29] ab288067 at a 1/2000 (0.315 ug/ml) dilution, P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 (0.013 ug/ml) dilution and GFAP with Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 at 1/1000 ( 1.325 ug/ml) dilution.

  Panel A: merged staining of anti-THP2 (green; Opal™520), anti-P2Y12 (grey; Opal™570) and anti-GFAP (magenta; Opal™690) on mouse midbrain.
  Panel B: anti-THP2 staining the serotonergic neurons in mouse midbrain.
  Panel C: anti-P2Y12 staining microglia in mouse midbrain.
  Panel D: anti-GFAP staining astrocytes in mouse midbrain.
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of ab2888067, Anti-P2Y12 antibody [EPR26298-93] ab300140 and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 (0.013 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on microglial cells in human cerebrum (PMID: 30196821). The section was incubated with Anti-P2Y12 antibody [EPR26298-93] ab300140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 (0.013 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on microglial cells in mouse cerebrum (PMID: 30196821). The section was incubated with Anti-P2Y12 antibody [EPR26298-93] ab300140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 (0.013 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on microglial cells in rat cerebrum (PMID: 30196821). The section was incubated with Anti-P2Y12 antibody [EPR26298-93] ab300140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 (0.013 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on human liver. The section was incubated with Anti-P2Y12 antibody [EPR26298-93] ab300140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Immunohistochemistry (Frozen sections) - Anti-P2Y12 antibody [EPR26298-93] - BSA and Azide free (ab300141)

  This data was developed using Anti-P2Y12 antibody [EPR26298-93] ab300140, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/500 (1.052 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.