AB140862

Anti-P2Y12 antibody - N-terminal

Name: Anti-P2Y12 antibody - N-terminal (ab140862) | Abcam
Brand: Abcam Limited
SKU: AB140862
Price: 630 USD
Availability: InStock

(1 Review)

(2 Publications)

Rabbit Polyclonal P2Y12 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human P2RY12.

View Alternative Names

HORK3, P2RY12, P2Y purinoceptor 12, P2Y12, ADP-glucose receptor, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, SP1999, ADPG-R, P2Y(ADP)

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody - N-terminal (AB140862)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody - N-terminal (AB140862)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human blood platelet tissue labelling P2Y12 with ab140862 at 32 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Synthetic Peptide within Human P2RY12. The exact immunogen used to generate this antibody is proprietary information.

Q9H244

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "32 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P2Y12 also referred to as the P2Y12 receptor is a G-protein coupled receptor with an approximate mass of 39.6 kDa. It plays a distinct role in platelet aggregation through ADP binding which activates intracellular signaling pathways that result in platelet activation. One finds P2Y12 expressed mainly in platelets and the brain. It is an important target of various antiplatelet drugs which highlights its critical role in physiological frameworks involving thrombosis.

Biological function summary

The P2Y12 receptor is an important component of the platelet activation pathway. When activated it contributes to the amplification of platelet responses to injury. P2Y12 does not function in isolation; it forms part of larger multi-protein complexes essential for thrombus formation. This aggregation process requires synchronized activation of several platelet receptors emphasizing the P2Y12 receptor's critical role in maintaining hemostasis through cellular signaling.

Pathways

The P2Y12 receptor is integral to the thrombin and ADP signaling pathways functioning in tandem with other proteins such as the P2Y1 and CXCR4 receptors to execute cellular responses. These pathways facilitate platelet shape change secretion and aggregation therefore solidifying their place within the broader scope of hemostatic responses. Coordination with such protein partners ensures efficient propagation of signals necessary for rapid platelet activation and clot formation.

P2Y12 is closely linked to cardiovascular diseases particularly thrombosis and myocardial infarction. Its involvement in excessive platelet aggregation makes it a significant target for therapeutic agents like the P2Y12 receptor blockers. The receptor also interacts with proteins like glycoprotein IIb/IIIa in the clotting process providing potential pathways for therapeutic intervention. Dysregulation of P2Y12 function presents a risk for unwarranted thrombotic events highlighting the importance of P2Y12 antagonists such as ticagrelor in clinical settings to manage such conditions effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation.

See full target information P2RY12

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5300 PubMed37652903

2023

Purinergic signaling mediates neuroglial interactions to modulate sighs.

Applications

Unspecified application

Species

Unspecified reactive species

Liza J Severs,Nicholas E Bush,Lely A Quina,Skyler Hidalgo-Andrade,Nicholas J Burgraff,Tatiana Dashevskiy,Andy Y Shih,Nathan A Baertsch,Jan-Marino Ramirez

Translational psychiatry 13:48 PubMed36750547

2023

A molecular characterization and clinical relevance of microglia-like cells derived from patients with panic disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Jung You,Chan Rim,Minji Bang,Soyoung Sung,Hui-Ju Kim,Sang-Hyuk Lee,Min-Soo Kwon

View all publications

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