Anti-P2Y14 antibody

• WB

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Western blot - Anti-P2Y14 antibody (AB136264)

All lanes:

Western blot - Anti-P2Y14 antibody (ab136264) at 1/500 dilution

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K562 cell lysate at 30 µg

Predicted band size: 39 kDa

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• WB

CiteAb

Western blot - Anti-P2Y14 antibody (AB136264)

P2Y14 western blot using anti-P2Y14 antibody ab136264. Publication image and figure legend from Ma, J., Wei, K., et al., 2020, Nat Commun, PubMed 32286295.

ab136264 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab136264 please see the product overview.

UDPG regulates inflammatory macrophages via P2Y14 receptor.a–cPgm1, Ugp2 or Gys1 siRNA transfected BMDMs were stimulated with IFN-γ/LPS for 24 h, Nos2, Tnf, Il6 and Il1b expression was determined by real-time PCR. dGys1, Pgm1 or Ugp2 siRNA transfected BMDMs were stimulated with IFN-γ/LPS for 24 h, UDPG in supernatants was determined by ELISA. e IFN-γ/LPS-stimulated BMDMs were treated with UDPG (0, 100 or 200 μM) for 24 or 36 h, Nos2, Tnf, Il6, and Il1b expression was determined by real-time PCR (left), iNOS, TNF and IL-6 expression was determined by western blot (middle) and ELISA (right). f, gUgp2 or G6pdx siRNA transfected BMDMs were stimulated with IFN-γ/LPS ± UDPG (200 μM) for 24 or 36 h, Nos2, Tnf, Il6, and Il1b expression was determined by real-time PCR (left), iNOS, TNF and IL-6 expression was determined by western blot (middle) and ELISA (right). hPygl or G6pdx siRNA transfected BMDMs were stimulated with IFN-γ/LPS for 24 h, UDPG in supernatants was determined by ELISA. i BMDMs were pretransfected with siRNA (Pygl or G6pdx) for 24 h prior to stimulation with IFN-γ/LPS for 6 h and switched to 13C-glucose for 6 h, lysed cells were analyzed by LC-MS/MS to determine m + 6-labeled UDPG. j, k P2Y14 receptor expression in untreated, IFN-γ/LPS- or IL-4-treated BMDMs was determined by real-time PCR (j) and western blot (k). l, m IFN-γ/LPS-stimulated BMDMs were treated with UDPG (0, 100 or 200 μM) for 24 or 36 h, P2Y14 expression was determined by real-time PCR (l) and western blot (m). n–pP2ry14 siRNA transfected BMDMs were stimulated with or without IFN-γ/LPS for 24 or 36 h, Nos2, Tnf, Il6, and Il1b expression was determined by real-time PCR (n), iNOS, TNF, and IL-6 expression was determined by western blot (o) and ELISA (p). Unless otherwise specified, n = 3 biologically independent experiments were performed. Data are presented as mean ± SEM. P values were calculated using one-way ANOVA. **p < 0.01, ****p < 0.0001.

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