Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free
- RabMAb
- Recombinant
- KO Validated
- What is this?
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(8 Publications)
Rabbit Recombinant Monoclonal p38 beta/MAPK11 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples. Cited in 8 publications.
View Alternative Names
PRKM11, SAPK2, SAPK2B, MAPK11, Mitogen-activated protein kinase 11, MAP kinase 11, MAPK 11, Mitogen-activated protein kinase p38 beta, Stress-activated protein kinase 2b, p38-2, MAP kinase p38 beta, p38b, SAPK2b
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (AB227995)
This IHC data was generated using the same anti-p38 antibody clone, Y122, in a different buffer formulation (cat# ab32142).
ab32142, at a 1/100 dilution, staining p38 in paraffin embedded human skin carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (AB227995)
Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling p38 (green) with purified ab32142 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
Secondary Only Control : PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32142).
- WB
Lab
Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (AB227995)
This data was developed using the same antibody clone in a different buffer formulation (ab32142).
Lanes 1 - 4 : Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab32142 was shown to react with p38 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (<a href='/en-us/products/primary-antibodies/p38-beta-mapk11-p38-alpha-mapk14-antibody-y122-ab32142'>ab32142</a>) at 1/1000 dilution
Lane 1:
HeLa cell lysate at 20 µg
Lane 2:
Jurkat cell lysate at 20 µg
Lane 2:
Western blot - Human MAPK14 (p38) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-mapk14-p38-knockout-hek-293t-cell-line-ab255406'>ab255406</a>)
Lane 3:
Wild-type HEK-293T cell lysate at 20 µg
Lane 4:
MAPK14 knockout HEK-293T cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 124 kDa,42 kDa
false
- WB
Unknown
Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (AB227995)
This data was developed using the same antibody clone in a different buffer formulation (ab32142).
All lanes:
Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (<a href='/en-us/products/primary-antibodies/p38-beta-mapk11-p38-alpha-mapk14-antibody-y122-ab32142'>ab32142</a>) at 1/1000 dilution
Lane 1:
Western blot - Recombinant Human p38 beta/MAPK11 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-p38-beta-mapk11-protein-ab117219'>ab117219</a>)
Lane 2:
Western blot - Recombinant Human p38 gamma/MAPK12 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-p38-gamma-mapk12-protein-ab117221'>ab117221</a>)
Lane 3:
Western blot - Recombinant Human p38 delta/MAPK13 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-p38-delta-mapk13-protein-ab113869'>ab113869</a>)
Lane 4:
Western blot - Recombinant Human p38 alpha/MAPK14 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-p38-alpha-mapk14-protein-ab82188'>ab82188</a>)
false
- WB
Lab
Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (AB227995)
This WB data was generated using the same anti-p38 antibody clone, Y122, in a different buffer formulation (cat# ab32142).
Lanes 1 - 4 : Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32142 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (<a href='/en-us/products/primary-antibodies/p38-beta-mapk11-p38-alpha-mapk14-antibody-y122-ab32142'>ab32142</a>)
Lane 1:
Wild-type HAP1 cell lysate at 20 µg
Lane 2:
p38 knockout HAP1 cell lysate at 20 µg
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
false
Related conjugates and formulations (3)
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Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122]
Reactivity data
Product details
ab227995 is the carrier-free version of ab32142.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P38 beta and p38 alpha functions include regulation of inflammation cell differentiation and apoptosis. They operate as part of a larger protein kinase complex and are activated by environmental stressors such as cytokines and UV radiation. This activation leads to a cascade of downstream signaling events modulating cellular processes essential for maintaining homeostasis. They have significant roles in immune response and stress-induced transcriptional regulation.
Pathways
These proteins primarily contribute to the MAPK signaling pathway and the stress-activated protein kinase (SAPK) pathway. The MAPK pathway also includes other kinases like JNK and ERK which work together to regulate cellular responses to a diverse array of stimuli. In the context of immune response these proteins play a critical role working in tandem with other kinases to facilitate cellular adaptation to stress and inflammation.
Product protocols
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Target data
Additional targets
Publications (8)
Recent publications for all applications. Explore the full list and refine your search
Oncotarget 8:26702-26717 PubMed28460458
2017
Applications
WB
Species
Unspecified reactive species
Evidence-based complementary and alternative medic 2015:307594 PubMed25802536
2015
Applications
Unspecified application
Species
Mouse
Cell death & disease 5:e1426 PubMed25255219
2014
Applications
Unspecified application
Species
Unspecified reactive species
Biochimica et biophysica acta 1843:2631-44 PubMed25072751
2014
Applications
WB
Species
Unspecified reactive species
PloS one 9:e98981 PubMed24901319
2014
Applications
WB
Species
Human
Journal of immunology (Baltimore, Md. : 1950) 191:594-607 PubMed23752610
2013
Applications
Unspecified application
Species
Mouse
Biochemical and biophysical research communication 420:17-23 PubMed22390933
2012
Applications
WB
Species
Human
BMC cell biology 11:10 PubMed20109185
2010
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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