AB251440

Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal p38 gamma/MAPK12 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Rat samples.

View Alternative Names

ERK6, SAPK3, MAPK12, Mitogen-activated protein kinase 12, MAP kinase 12, MAPK 12, Extracellular signal-regulated kinase 6, Mitogen-activated protein kinase p38 gamma, Stress-activated protein kinase 3, ERK-6, MAP kinase p38 gamma

7 Images

Supplier Data

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

This data was developed using ab205926, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (<a href='/en-us/products/primary-antibodies/p38-gamma-mapk12-antibody-epr6528n-ab205926'>ab205926</a>) at 1/10000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Lane 3:

Human fetal liver tissue lysate at 20 µg

Lane 4:

Human fetal skin tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

This data was developed using ab205926, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-2 : 3 minutes; Lane 3-4 : 1 minute; Lane 5 : 30 seconds.

All lanes:

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (<a href='/en-us/products/primary-antibodies/p38-gamma-mapk12-antibody-epr6528n-ab205926'>ab205926</a>) at 1/1000 dilution

Lane 1:

Rat heart tissue lysate at 10 µg

Lane 2:

Rat spleen tissue lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 5:

Rat muscle tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

This data was developed using ab205926, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A673 (Human muscle Ewing's Sarcoma cell line) cells labeling MAPK 12 with ab205926 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on A673 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab205926 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

This data was developed using ab205926, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling MAPK 12 with ab205926 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab205926 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Lab

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

This data was developed using ab205926, the same antibody clone in a different buffer formulation.

Lanes 1-3 : Merged signal (red and green). Green - ab205926 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.

ab205926 Anti-MAPK 12 antibody [EPR6528(N)] was shown to specifically react with MAPK 12 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266280 (knockout cell lysate ab258041) was used. Wild-type and MAPK 12 knockout samples were subjected to SDS-PAGE. ab205926 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (<a href='/en-us/products/primary-antibodies/p38-gamma-mapk12-antibody-epr6528n-ab205926'>ab205926</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

MAPK12 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human MAPK12 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-mapk12-knockout-hek-293t-cell-line-ab266280'>ab266280</a>)

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Lab

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

This data was developed using ab205926, the same antibody clone in a different buffer formulation.

Lane 1 : Wild type HAP1 whole cell lysate (0 Âμg)

Lane 2 : empty knockout HAP1 whole cell lysate (20 Âμg)

Lane 3 : MAPK 12 whole cell lysate (0 Âμg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab205926 observed at 45 kDa. Red - loading control, ab18058, observed at 124 kDa.

All lanes:

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (<a href='/en-us/products/primary-antibodies/p38-gamma-mapk12-antibody-epr6528n-ab205926'>ab205926</a>)

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

This data was developed using ab205926, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (<a href='/en-us/products/primary-antibodies/p38-gamma-mapk12-antibody-epr6528n-ab205926'>ab205926</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Unconjugated

Anti-p38 gamma/MAPK12 antibody [EPR6528(N)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6528(N)

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251440 is the carrier-free version of ab205926.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p38 gamma protein also known as MAPK12 is a serine/threonine-protein kinase belonging to the mitogen-activated protein kinase (MAPK) family. It has a molecular mass of approximately 41 kDa. This protein is heavily expressed in skeletal muscle and heart tissues although expression occurs in other tissues at lower levels. p38 gamma plays a mechanical role in response to stress signals by phosphorylating downstream targets affecting cellular responses such as proliferation and differentiation.

Biological function summary

P38 gamma/MAPK12 influences the regulation of gene expression apoptosis and cell cycle processes. It operates within a specific MAP kinase signaling module that conveys signals from the cellular surface to the nucleus. This protein sometimes works in conjunction with other members of the MAPK family forming complexes that further fine-tune cellular responses under various conditions. Its activities help cells adapt to changes in their environment by modulating transcription factors and other important substrates.

Pathways

The MAPK pathway and the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) pathway both involve p38 gamma/MAPK12. Within these pathways p38 gamma is closely related to proteins such as JNK1 and JNK2 which also respond to stress signals. These pathways are essential for transmitting signals that regulate cellular growth differentiation and stress responses revealing intricacies of cellular adaptation to the environment.

Aberrant activation or dysfunction of p38 gamma/MAPK12 relates to conditions like cancer and cardiac hypertrophy. In cancer p38 gamma can influence tumor progression and metastasis through its impact on cell proliferation and survival. It also interacts with other proteins like MKK3 and MKK6 which serve as upstream activators in oncogenic signaling pathways. In cardiac hypertrophy altered p38 gamma signaling affects cardiac muscle cell growth potentially leading to heart failure. Understanding these connections provides insight into the mechanisms underlying these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes; and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.

See full target information MAPK12

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] - BSA and Azide free (AB251440)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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