AB122517

Anti-p38IP antibody

Name: Anti-p38IP antibody (ab122517) | Abcam
Brand: Abcam Limited
SKU: AB122517

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(3 Publications)

Rabbit Polyclonal p38IP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Transfected cell lysate samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SUPT20H aa 50-200.

View Alternative Names

C13orf19, FAM48A, FP757, SUPT20H, Transcription factor SPT20 homolog, p38-interacting protein, p38IP

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (AB122517)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (AB122517)

Paraffin embedded human cerebral cortex tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-p38IP antibody (AB122517)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p38IP antibody (AB122517)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122517 1-4 µg/ml. Cells treated with PFA/Triton X-100.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (AB122517)

Paraffin embedded human rectum tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (AB122517)

Paraffin embedded human testis tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (AB122517)

Paraffin embedded human prostate tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-p38IP antibody (AB122517)

All lanes:

Western blot - Anti-p38IP antibody (ab122517)

Lane 1:

Marker

Lane 2:

HEK293T cell lysate - vector only transfected

Lane 3:

HEK293T cell lysate - overexpressing p38IP with a C-terminal myc-DDK tag (~3.1kDa)

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SUPT20H aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q8NEM7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P38-interacting protein (p38IP) functions mechanically as a scaffold protein that interacts with p38 MAPK aiding in its regulation and stabilization. It is occasionally referred to as MO25. The molecular mass of p38IP is approximately 50 kDa. Expression of p38IP is observed in various tissues with notably high levels in the brain and the heart. This distribution suggests its involvement in processes across different cell types.

Biological function summary

This protein plays a role in cellular signaling and stress responses. p38IP does not function alone; it is a component of the p38 MAPK signaling complex. By interacting with other members of this pathway such as MKK3 and MKK6 p38IP modulates cellular responses to external stressors. Its involvement in the signaling process highlights its contribution to the control of cellular dynamics under stress.

Pathways

P38IP holds significance in the MAPK signaling pathway and the JNK signaling pathway. In the MAPK signaling pathway it interfaces closely with p38 MAPK and other kinases facilitating signal transduction that impacts processes like inflammation and apoptosis. In the JNK signaling pathway interactions with JNK MAPKs suggest a role in regulating gene expression in response to stress stimuli. These pathways underline the regulatory capacity of p38IP in controlling cellular fate during stress conditions.

P38IP associates with cancer progression and inflammatory disorders. Abnormal expression or malfunction of p38IP can contribute to dysregulated cell signaling in cancer partly through its relationship with p38 MAPK a known mediator of cell proliferation. In inflammatory disorders alterations in p38IP levels can affect immune response signaling also mediated through its partner JNK MAPKs illustrating its potential as a therapeutic target in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail (By similarity). Required for starvation-induced ATG9A trafficking during autophagy.

See full target information SUPT20H

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 14:1104153 PubMed36778009

2023

Sesquiterpene lactones-enriched fractions from Kitag alleviate RA by regulating M1 macrophage polarization NF-κB and MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Han,Jingwen Wang,Yicun Wang,Zhiqi Zhu,Siwang Zhang,Bingrong Wu,Mingsong Meng,Jianning Zhao,Dongsheng Wang

Journal for immunotherapy of cancer 10: PubMed35768165

2022

Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Zhao,Yuelong Wang,Nian Yang,Min Mu,Zhiguo Wu,Hexian Li,Xin Tang,Kunhong Zhong,Zongliang Zhang,Cheng Huang,Ting Cao,Meijun Zheng,Guoqing Wang,Chunlai Nie,Hui Yang,Gang Guo,Liangxue Zhou,Xi Zheng,Aiping Tong

Biology of reproduction 100:896-906 PubMed30535233

2018

Inhibition of Skp1-Cullin-F-box complexes during bovine oocyte maturation and preimplantation development leads to delayed development of embryos†.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Kinterova,Jiri Kanka,Veronika Petruskova,Tereza Toralova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p38IP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (AB122517)

Immunocytochemistry/ Immunofluorescence - Anti-p38IP antibody (AB122517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (AB122517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (AB122517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38IP antibody (AB122517)

Western blot - Anti-p38IP antibody (AB122517)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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