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AB248345

Anti-p39 antibody [EPR5075(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal p39 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.
2 Images
Western blot - Anti-p39 antibody [EPR5075(2)] - BSA and Azide free (AB248345)
  • WB

Unknown

Western blot - Anti-p39 antibody [EPR5075(2)] - BSA and Azide free (AB248345)

This data was developed using ab129196, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-p39 antibody [EPR5075(2)] (<a href='/en-us/products/primary-antibodies/p39-antibody-epr50752-ab129196'>ab129196</a>) at 1/1000 dilution

Lane 1:

Neuro 2a lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

SH-SY5Y lysate at 10 µg

Lane 4:

U-87 MG lysate at 10 µg

Lane 5:

Human oligodendroglioma lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 39 kDa

false

OI-RD Scanning - Anti-p39 antibody [EPR5075(2)] - BSA and Azide free (AB248345)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-p39 antibody [EPR5075(2)] - BSA and Azide free (AB248345)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5075(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab248345 is the carrier-free version of ab129196.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein p39 also known as cyclin Y is a protein with a mass of approximately 39 kDa. It plays a role in cell cycle regulation. Expression of p39 occurs mainly in neural tissues where it participates in control of neuronal development and synaptic function. In addition p39 associates with cell membranes indicating its potential involvement in signal transduction pathways.
Biological function summary

P39 functions as a regulatory partner in cyclin-dependent kinase (CDK) complexes. These complexes are essential during various phases of the cell cycle including G1/S transition and mitosis. By forming complexes with CDK5 p39 modulates neuronal processes and affects aspects such as neuronal migration and axonal guidance. Moreover p39 interacts with other signaling molecules potentially influencing synaptic plasticity.

Pathways

Research shows p39's integration within the cell cycle and neuronal signaling pathways. In cell cycle regulation it interacts with proteins like CDK5 to influence cellular proliferation and differentiation. Additionally p39 plays a role in the Wnt signaling pathway where it could affect cellular growth and differentiation through interactions with key proteins like GSK-3β.

P39 associates with neurodegenerative conditions including Alzheimer’s disease. Abnormal p39 expression levels may disrupt normal neuronal function and homeostasis leading to disease progression. The protein's connection to CDK5 which is implicated in Alzheimer’s highlights the significance of p39 as a potential therapeutic target. Furthermore irregularities in Wnt signaling linked to p39 may contribute to the development of other neurological disorders suggesting important roles for p39 in maintaining neural integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activator of CDK5/TPKII.
See full target information CDK5R2

Alternative Names

NCK5AI, CDK5R2, Cyclin-dependent kinase 5 activator 2, CDK5 activator 2, Cyclin-dependent kinase 5 regulatory subunit 2, p39, p39I
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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Cell proliferation 56:e13400 PubMed36642844

2023

Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Weiqiang Ruan,Chunhao Guo,Ke Chen,Le Li,Jihua Tian,Zhiquan Hu,Dan Peng,Xing Zeng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com