AB137691

Anti-p40 antibody

Name: Anti-p40 antibody (ab137691) | Abcam
Brand: Abcam Limited
SKU: AB137691

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(4 Publications)

Rabbit Polyclonal p40 antibody. Suitable for IHC-P, WB, ICC/IF, IP and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RABEPK aa 150 to C-terminus.

View Alternative Names

RAB9P40, RABEPK, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein, p40

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 antibody (AB137691)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 antibody (AB137691)

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab137691 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-p40 antibody (AB137691)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p40 antibody (AB137691)

Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab137691 at 1/200 dilution.

Supplier Data

Immunoprecipitation - Anti-p40 antibody (AB137691)

Immunoprecipitation of p40 protein from A431 whole cell extracts using 5 µg of ab137691.
Western blot analysis was performed using ab137691.

All lanes:

Immunoprecipitation - Anti-p40 antibody (ab137691) at 5 µg

Lane 1:

A431 whole cell extracts with control IgG

Lane 2:

Immunoprecitated A431 whole cell extracts using 5 µg of ab137691

Secondary

All lanes:

Anti Rabbit IgG

Predicted band size: 41 kDa

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Western blot - Anti-p40 antibody (AB137691)

10% SDS PAGE

All lanes:

Western blot - Anti-p40 antibody (ab137691) at 1/1000 dilution

All lanes:

whole cell lysate prepared from HepG2 cells at 30 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human RABEPK aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7Z6M1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Or Tris-EDTA buffer pH8.0 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p40 protein also known as IL-12/IL-23 p40 subunit possesses a molecular mass of approximately 40 kDa. Scientists often detect p40 in immune cells such as dendritic cells and macrophages. This subunit exists as part of two heterodimeric cytokines IL-12 and IL-23 making it an important component to study in both health and disease conditions. It plays a significant role in mediating immune responses which makes it a vital target for research into immune system functioning.

Biological function summary

The p40 subunit forms part of the heterodimer complexes IL-12 and IL-23 which have different roles in the immune response. IL-12 which includes the p35 subunit alongside p40 is key for the differentiation of naive T cells into Th1 cells. This action leads to enhancement of the cellular immune response. In contrast the combination of p40 with p19 forms IL-23 which supports the survival and proliferation of Th17 cells playing a unique role in maintaining tissue integrity and combating extracellular pathogens.

Pathways

The p40 protein interacts significantly in the IL-12 and IL-23 signaling pathways which are essential for immune regulation and response. In these pathways IL-12 and IL-23 bind to their respective receptors leading to downstream effects that encourage the activation and differentiation of T cells. The signaling pathways involve other proteins such as STAT4 important for IL-12-dependent responses and STAT3 which is important for IL-23-mediated pathways. These interactions show how p40 contributes to complex and critical immune network regulation.

The p40 protein features prominently in autoimmune diseases and chronic inflammatory conditions. Researchers have linked aberrant production of IL-12 and IL-23 to conditions like psoriasis and inflammatory bowel disease. Elevated levels of p40 often p40-positive can align with disease states wherein the immune system mistakenly attacks the body’s own cells. This association with disease processes makes p40 a target for therapies aiming to modulate immune response. In the case of disorders proteins such as IL-23 and its receptor are involved alongside p40 further emphasizing its key role in pathological immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Rab9 effector required for endosome to trans-Golgi network (TGN) transport.

See full target information RABEPK

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 17:3817-3825 PubMed30881502

2019

Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method.

Applications

Unspecified application

Species

Unspecified reactive species

Yunpeng Zhao,Shukang Zhao,Yingjie Chen,Xiaopeng Dong,Chuanliang Peng,Qifeng Sun,Lei Shan,Zhendan Wang,Xiaogang Zhao

Pathology international 67:171-175 PubMed28150468

2017

Ciliated muconodular papillary tumor of the lung harboring ALK gene rearrangement: Case report and review of the literature.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Jin,Xuxia Shen,Lei Shen,Yihua Sun,Haiquan Chen,Yuan Li

Journal of immunology (Baltimore, Md. : 1950) 198:1985-1994 PubMed28093527

2017

Human Cystic Fibrosis Macrophages Have Defective Calcium-Dependent Protein Kinase C Activation of the NADPH Oxidase, an Effect Augmented by .

Applications

Unspecified application

Species

Unspecified reactive species

Kaivon Assani,Chandra L Shrestha,Frank Robledo-Avila,Murugesan V Rajaram,Santiago Partida-Sanchez,Larry S Schlesinger,Benjamin T Kopp

Traffic (Copenhagen, Denmark) 17:211-29 PubMed26663757

2016

Spatiotemporal Resolution of Rab9 and CI-MPR Dynamics in the Endocytic Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Kucera,Marita Borg Distefano,Axel Berg-Larsen,Frode Skjeldal,Urska Repnik,Oddmund Bakke,Cinzia Progida

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p40 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 antibody (AB137691)

Immunocytochemistry/ Immunofluorescence - Anti-p40 antibody (AB137691)

Immunoprecipitation - Anti-p40 antibody (AB137691)

Western blot - Anti-p40 antibody (AB137691)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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