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Rabbit Polyclonal p40 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Rab9 effector protein with kelch motifs aa 150 to C-terminus.

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Images

Western blot - Anti-p40 antibody (AB137691), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-p40 antibody (AB137691), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 antibody (AB137691), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Rab9 effector protein with kelch motifs aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z6M1

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

Or Tris-EDTA buffer pH8.0

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Target data

Function

Rab9 effector required for endosome to trans-Golgi network (TGN) transport.

Alternative names

Recommended products

Rabbit Polyclonal p40 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Rab9 effector protein with kelch motifs aa 150 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Rab9 effector protein with kelch motifs aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z6M1
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The p40 protein also known as IL-12/IL-23 p40 subunit possesses a molecular mass of approximately 40 kDa. Scientists often detect p40 in immune cells such as dendritic cells and macrophages. This subunit exists as part of two heterodimeric cytokines IL-12 and IL-23 making it an important component to study in both health and disease conditions. It plays a significant role in mediating immune responses which makes it a vital target for research into immune system functioning.

Biological function summary

The p40 subunit forms part of the heterodimer complexes IL-12 and IL-23 which have different roles in the immune response. IL-12 which includes the p35 subunit alongside p40 is key for the differentiation of naive T cells into Th1 cells. This action leads to enhancement of the cellular immune response. In contrast the combination of p40 with p19 forms IL-23 which supports the survival and proliferation of Th17 cells playing a unique role in maintaining tissue integrity and combating extracellular pathogens.

Pathways

The p40 protein interacts significantly in the IL-12 and IL-23 signaling pathways which are essential for immune regulation and response. In these pathways IL-12 and IL-23 bind to their respective receptors leading to downstream effects that encourage the activation and differentiation of T cells. The signaling pathways involve other proteins such as STAT4 important for IL-12-dependent responses and STAT3 which is important for IL-23-mediated pathways. These interactions show how p40 contributes to complex and critical immune network regulation.

Associated diseases and disorders

The p40 protein features prominently in autoimmune diseases and chronic inflammatory conditions. Researchers have linked aberrant production of IL-12 and IL-23 to conditions like psoriasis and inflammatory bowel disease. Elevated levels of p40 often p40-positive can align with disease states wherein the immune system mistakenly attacks the body’s own cells. This association with disease processes makes p40 a target for therapies aiming to modulate immune response. In the case of disorders proteins such as IL-23 and its receptor are involved alongside p40 further emphasizing its key role in pathological immune responses.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Western blot - Anti-p40 antibody (ab137691), expandable thumbnail

    Western blot - Anti-p40 antibody (ab137691)

    10% SDS PAGE

    All lanes: Western blot - Anti-p40 antibody (ab137691) at 1/1000 dilution

    All lanes: whole cell lysate prepared from HepG2 cells at 30 µg

    Predicted band size: 41 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-p40 antibody (ab137691), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-p40 antibody (ab137691)

    Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab137691 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 antibody (ab137691), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 antibody (ab137691)

    Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab137691 at 1/100 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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