Anti-p40 antibody [EPR13781]

• WB

Lab

Western blot - Anti-p40 antibody [EPR13781] (AB184173)

Western blot : Anti-p40 antibody [EPR13781] ab184173 staining at 1/2000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type A549 cell lysates with no signal observed at this size in RABEPK knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-p40 antibody [EPR13781] (ab184173) at 1/2000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

RABEPK knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 40 kDa,51 kDa

false

• WB

Supplier Data

Western blot - Anti-p40 antibody [EPR13781] (AB184173)

5% NFDM/TBST blocking/dilution buffer

All lanes:

Western blot - Anti-p40 antibody [EPR13781] (ab184173) at 1/2000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

U87-MG cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false