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AB203826

Anti-p40 - DeltaNp63 antibody [EPR17863-47]

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(8 Publications)

Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826) is a rabbit monoclonal antibody detecting p40 - DeltaNp63 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

KET, P63, P73H, P73L, TP73L, TP63, Tumor protein 63, p63, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p40, p51, CUSP, p73L

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the basal cells of the human breast tissue is observed (PMID : 11839669; PMID : 28303349) The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma and adenocarcinoma tissue labeling p40 - DeltaNp63 with ab203826 at a 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin.

Nuclear staining on the squamous cell carcinoma (Panel A), negative staining on the adenocarcinoma (Panel B) is observed (PMID : 23486764).

The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Flow Cytometry (Intracellular) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HaCaT (Human skin keratinocyte, Right) cells labeling p40 - DeltaNp63 with ab203826 at 1/400 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) at 1/2000 was used as the secondary antibody.

Negative control : HeLa. (PMID : 21631996).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the epidermis of the human skin is observed (PMID : 11839669). The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (human skin keratinocyte) HeLa (human cervix adenocarcinoma epithelial cell) cells labeling p40 - DeltaNp63 with ab203826 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear and weak cytoplasmic staining in HaCaT cells.

Negative control : HeLa (PMID : 21631996).

ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the epidermis of the rat skin is observed. The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the epidermis of the mouse skin is observed. The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Western blot - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)
  • WB

Lab

Western blot - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HeLa (PMID : 21631996).

The molecular weight observed is consistent with what has been described in the literature (PMID : 17615180, 26784942).

Exposure time : Lane 37 seconds; Lanes 2-3 : 3 minutes.

All lanes:

Western blot - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826) at 1/1000 dilution

Lane 1:

HaCaT (human skin keratinocyte) whole cell lysate at 40 µg

Lane 2:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 77 kDa

Observed band size: 75 kDa

false

Immunoprecipitation - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)
  • IP

Unknown

Immunoprecipitation - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (AB203826)

p40 - DeltaNp63 was immunoprecipitated from 0.35 mg HaCaT (Human skin keratinocyte) whole cell lysate 10μg with ab203826 at 1/30 dilution (2μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab203826 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HaCaT whole cell lysate 10μg.

Lane 2 : ab203826 IP in HaCaT whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203826 in HaCaT whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

Predicted band size: 53 kDa

Observed band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17863-47

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, Flow Cyt (Intra), IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of p40 - DeltaNp63?
Anti-p40 - DeltaNp63 [EPR17863-47] (ab203826) specifically detects a band for p40 - DeltaNp63 (UniProt: Q9H3D4) at a molecular weight of 77kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR17863-47] also available for your convenience: ab203826, Carrier free - ab269956, Alexa Fluor® 647 - ab275134

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target p40 also known as ΔNp63 is an essential protein isoform of the p63 gene. This target weighs approximately 40 kDa and is expressed predominantly in epithelial tissues such as the basal layer of the skin and select organs. Due to its significant role in development and differentiation researchers often study p40 in the context of specific cancers using techniques like p40 immunostaining p40 IHC and p40 marker assays including the anti-p40 stain.
Biological function summary

The p40 protein acts as a transcription factor regulating epithelial stem cell maintenance and differentiation. It is an integral component of the tetrameric complex of the p63 protein family where it influences the transcription of genes involved in cell cycle control and apoptotic pathways. The ΔNp63 variant lacking the N-terminal transactivation domain plays an important role in blocking premature senescence contributing to cell proliferative capacity.

Pathways

P40 is involved in the Notch signaling and the PI3K/AKT signaling pathways. In Notch signaling p40 interacts alongside related proteins like bcl-2 or B28 impacting the regulation of cell fate and differentiation. The PI3K/AKT pathway involves p40 in processes essential for cell survival and proliferation often overlapping with proteins such as AKT and mTOR.

P40 significantly links to various forms of cancer especially squamous cell carcinoma and lung adenocarcinoma. Aberrant expression of ΔNp63 and its disruption in pathways contribute to the pathogenesis of these cancers. In squamous cell carcinoma p40 expression serves as an important biomarker distinguishing it from other tumor types connecting with proteins like Bcl-2 which are important in managing apoptotic resistance in cancerous cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.
See full target information TP63
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6032 PubMed40595652

2025

EZH2 loss promotes gastric squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengsha Zhang,Ailing Zhong,Hongyu Liu,Lei Zhao,Yingjie Wang,Zhenghao Lu,Lanxin Zhang,Xiangyu Pan,Zuoyu Liang,Limin Gao,Xuelan Chen,Jian Wang,Siqi Dai,Xudong Wan,Manli Wang,Jingyao Chen,Jiajia Du,Shuang Chen,Tingfa Peng,Jingyang Cai,Liuxiang Chen,Yang Yang,Chaoxin Xiao,Menglin Yao,Jing Xu,Runkuan Qin,Chan Yang,Baohong Wu,Qi Zhang,Shengzhuo Liu,Chengjian Zhao,Hongxin Deng,Yuan Wang,Lu Chen,Jiankun Hu,Bing Hu,Shengyong Yang,Feifei Na,Linjie Guo,Yu Liu,Chong Chen

The Journal of clinical investigation 135: PubMed40454484

2025

Polybromo 1/vimentin axis dictates tumor grade, epithelial-mesenchymal transition, and metastasis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Munenori Kawai,Akihisa Fukuda,Munehiro Ikeda,Kei Iimori,Kenta Mizukoshi,Kosuke Iwane,Go Yamakawa,Mayuki Omatsu,Mio Namikawa,Makoto Sono,Tomonori Masuda,Yuichi Fukunaga,Munemasa Nagao,Osamu Araki,Takaaki Yoshikawa,Satoshi Ogawa,Yukiko Hiramatsu,Motoyuki Tsuda,Takahisa Maruno,Yuki Nakanishi,Dieter Saur,Tatsuaki Tsuruyama,Toshihiko Masui,Etsuro Hatano,Hiroshi Seno

Cancers 16: PubMed39199657

2024

Development of Ex Vivo Analysis for Examining Cell Composition, Immunological Landscape, Tumor and Immune Related Markers in Non-Small-Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Elena G Ufimtseva,Margarita S Gileva,Ruslan V Kostenko,Vadim V Kozlov,Lyudmila F Gulyaeva

International journal of biological sciences 19:2848-2859 PubMed37324952

2023

Liver X receptors and estrogen receptor β, two players in a rare subtype of NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Wanfu Wu,Mozhgan Sarhadi,Xiaoyu Song,Jingling Xue,Yubing Dai,Jan-Ake Gustafsson

iScience 26:106688 PubMed37187698

2023

Trigeminal nerve-derived substance P regulates limbal stem cells by the PI3K-AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Xu,Xiongshi Lin,Xing Dong,Ying Liu,Zhichong Wang,Shuangyong Wang

Cancer cell 41:88-105.e8 PubMed36525973

2022

KMT2D deficiency drives lung squamous cell carcinoma and hypersensitivity to RTK-RAS inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanwang Pan,Han Han,Hai Hu,Hua Wang,Yueqiang Song,Yuan Hao,Xinyuan Tong,Ayushi S Patel,Selim Misirlioglu,Sittinon Tang,Hsin-Yi Huang,Ke Geng,Ting Chen,Angeliki Karatza,Fiona Sherman,Kristen E Labbe,Fan Yang,Alison Chafitz,Chengwei Peng,Chenchen Guo,Andre L Moreira,Vamsidhar Velcheti,Sally C M Lau,Pengfei Sui,Haiquan Chen,J Alan Diehl,Anil K Rustgi,Adam J Bass,John T Poirier,Xiaoyang Zhang,Hongbin Ji,Hua Zhang,Kwok-Kin Wong

PloS one 15:e0244327 PubMed33373367

2020

Characterization of the secretory profile and exosomes of limbal stem cells in the canine species.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio J Villatoro,Cristina Alcoholado,María Del Carmen Martín-Astorga,Gustavo Rico,Viviana Fernández,José Becerra

Cancer management and research 12:13149-13159 PubMed33376406

2020

Pyrrolidine Dithiocarbamate Facilitates Arsenic Trioxide Against Pancreatic Cancer via Perturbing Ubiquitin-Proteasome Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Simin Yu,Ning Wu,Jianmin Zhu,Ying Liu,Jinbin Han
View all publications
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