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Rabbit Recombinant Monoclonal P63 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (AB269956), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (AB269956), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (AB269956), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (AB269956), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (AB269956), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Expected
Rat
Expected
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

1 product for Alternative Version

Target data

Function

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.

Alternative names

KET, P63, P73H, P73L, TP73L, TP63, Tumor protein 63, p63, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p40, p51, CUSP, p73L

Recommended products

Rabbit Recombinant Monoclonal P63 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR17863-47
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab269956 is the carrier-free version of Anti-p40 - DeltaNp63 antibody [EPR17863-47] ab203826.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The biological target p40 also known as ΔNp63 is an essential protein isoform of the p63 gene. This target weighs approximately 40 kDa and is expressed predominantly in epithelial tissues such as the basal layer of the skin and select organs. Due to its significant role in development and differentiation researchers often study p40 in the context of specific cancers using techniques like p40 immunostaining p40 IHC and p40 marker assays including the anti-p40 stain.

Biological function summary

The p40 protein acts as a transcription factor regulating epithelial stem cell maintenance and differentiation. It is an integral component of the tetrameric complex of the p63 protein family where it influences the transcription of genes involved in cell cycle control and apoptotic pathways. The ΔNp63 variant lacking the N-terminal transactivation domain plays an important role in blocking premature senescence contributing to cell proliferative capacity.

Pathways

P40 is involved in the Notch signaling and the PI3K/AKT signaling pathways. In Notch signaling p40 interacts alongside related proteins like bcl-2 or B28 impacting the regulation of cell fate and differentiation. The PI3K/AKT pathway involves p40 in processes essential for cell survival and proliferation often overlapping with proteins such as AKT and mTOR.

Associated diseases and disorders

P40 significantly links to various forms of cancer especially squamous cell carcinoma and lung adenocarcinoma. Aberrant expression of ΔNp63 and its disruption in pathways contribute to the pathogenesis of these cancers. In squamous cell carcinoma p40 expression serves as an important biomarker distinguishing it from other tumor types connecting with proteins like Bcl-2 which are important in managing apoptotic resistance in cancerous cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com