AB273142

Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal P63 antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TP63.

View Alternative Names

KET, P63, P73H, P73L, TP73L, TP63, Tumor protein 63, p63, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p40, p51, CUSP, p73L

3 Images

Flow Cytometry - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (AB273142)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (AB273142)

Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells using ab273135 followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273135).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (AB273142)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (AB273142)

Paraffin-embedded human kidney tissue stained for p40 (deltaNp63) using ab273135 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273135).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (AB273142)

Paraffin-embedded human skin tissue stained for p40 (deltaNp63) using ab273135 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273135).

Unconjugated

Anti-p40 - DeltaNp63 antibody [rTP40/3690]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

rTP40/3690

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human TP63. The exact immunogen used to generate this antibody is proprietary information.

Q9H3D4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

ab273142 is the carrier-free version of ab273135.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target p40 also known as ΔNp63 is an essential protein isoform of the p63 gene. This target weighs approximately 40 kDa and is expressed predominantly in epithelial tissues such as the basal layer of the skin and select organs. Due to its significant role in development and differentiation researchers often study p40 in the context of specific cancers using techniques like p40 immunostaining p40 IHC and p40 marker assays including the anti-p40 stain.

Biological function summary

The p40 protein acts as a transcription factor regulating epithelial stem cell maintenance and differentiation. It is an integral component of the tetrameric complex of the p63 protein family where it influences the transcription of genes involved in cell cycle control and apoptotic pathways. The ΔNp63 variant lacking the N-terminal transactivation domain plays an important role in blocking premature senescence contributing to cell proliferative capacity.

Pathways

P40 is involved in the Notch signaling and the PI3K/AKT signaling pathways. In Notch signaling p40 interacts alongside related proteins like bcl-2 or B28 impacting the regulation of cell fate and differentiation. The PI3K/AKT pathway involves p40 in processes essential for cell survival and proliferation often overlapping with proteins such as AKT and mTOR.

P40 significantly links to various forms of cancer especially squamous cell carcinoma and lung adenocarcinoma. Aberrant expression of ΔNp63 and its disruption in pathways contribute to the pathogenesis of these cancers. In squamous cell carcinoma p40 expression serves as an important biomarker distinguishing it from other tumor types connecting with proteins like Bcl-2 which are important in managing apoptotic resistance in cancerous cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.

See full target information TP63

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free

Flow Cytometry - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (AB273142)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (AB273142)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [rTP40/3690] - BSA and Azide free (AB273142)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise