AB231639

Anti-p40-phox antibody

Name: Anti-p40-phox antibody (ab231639) | Abcam
Brand: Abcam Limited
SKU: AB231639
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal p40-phox antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Neutrophil cytosol factor 4 aa 100 to C-terminus.

View Alternative Names

Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, p40-phox, p40phox, Ncf4

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Paraffin-embedded mouse liver tissue stained for p40-phox using ab231639 at 20 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Paraffin-embedded mouse kidney tissue stained for p40-phox using ab231639 at 20 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Paraffin-embedded mouse spleen tissue stained for p40-phox using ab231639 at 20 μg/ml in immunohistochemical analysis.

Supplier Data

Western blot - Anti-p40-phox antibody (AB231639)

All lanes:

Western blot - Anti-p40-phox antibody (ab231639) at 3 µg/mL

All lanes:

Mouse spleen lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-p40-phox antibody (AB231639)

All lanes:

Western blot - Anti-p40-phox antibody (ab231639) at 3 µg/mL

All lanes:

Recombinant mouse p40-phox protein.

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Neutrophil cytosol factor 4 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P97369

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231639 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P40-phox also known as neutrophil cytosolic factor 4 (NCF4) is a regulatory component of the NADPH oxidase complex. It is expressed in phagocytic cells such as neutrophils and macrophages. The protein plays a role in the production of reactive oxygen species (ROS) during the respiratory burst. The molecular mass of p40-phox is approximately 40 kDa. It interacts with other subunits of the NADPH oxidase complex such as p67-phox and p47-phox contributing to the assembly and activation of the complex.

Biological function summary

P40-phox participates in innate immune responses by regulating the NADPH oxidase complex's function. It acts as a scaffold to facilitate the interaction between cytosolic and membrane components. p40-phox integrates into the cytosolic fraction with other subunits and translocates to the membrane upon stimulation. This translocation is essential for generating superoxide radicals that are involved in defense against pathogens. Its functional domains enable it to bind phosphatidylinositol (34) bisphosphate which anchors the protein to the membrane. p40-phox itself is not an enzyme but modulates the activity of the complex.

Pathways

The p40 protein serves as an important player in the signaling cascade of the respiratory burst. It interacts with the phosphoinositide 3-kinase (PI3K) pathway and cooperates with other proteins like p67-phox and p47-phox through phosphorylation-dependent mechanisms. The p40-phox is phox compatible meaning it can integrate into various signaling modules. Within the oxidative burst pathway it enhances the cytosolic complex's attachment to the membrane affecting the production of ROS.

Mutations in the p40-phox have been connected to chronic granulomatous disease (CGD) characterized by recurrent infections and granuloma formation. The lack of functional p40-phox disrupts the assembly of the NADPH oxidase complex leading to decreased ROS production. This deficiency impairs the ability of phagocytes to eliminate pathogens effectively. p40-phox is also related to inflammatory disorders like inflammatory bowel disease (IBD) as variations in the NCF4 gene affect the immune response. The dysfunction in ROS production impacts the signaling involving p67-phox and p47-phox further influencing disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.

See full target information Neutrophil cytosol factor 4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed35625704

2022

Arsenic Activates the ER Stress-Associated Unfolded Protein Response via the Activating Transcription Factor 6 in Human Bronchial Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Wadgaonkar,Zhuoyue Bi,Junmei Wan,Yao Fu,Qian Zhang,Bandar Almutairy,Wenxuan Zhang,Yiran Qiu,Chitra Thakur,Maik Hüttemann,Fei Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p40-phox antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody (AB231639)

Western blot - Anti-p40-phox antibody (AB231639)

Western blot - Anti-p40-phox antibody (AB231639)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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