Rabbit Recombinant Monoclonal p40-phox antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (AB247448), expandable thumbnail](https://content.abcam.com/products/images/p40-phox-antibody-ep2142y-bsa-and-azide-free-ab247448--western-blot-img137020.gif "Western blot - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (AB247448)")
![Immunocytochemistry/ Immunofluorescence - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (AB247448), expandable thumbnail](https://content.abcam.com/products/images/p40-phox-antibody-ep2142y-bsa-and-azide-free-ab247448--immunocytochemistry-immunofluorescence-img131871.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (AB247448)")
![Immunoprecipitation - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (AB247448), expandable thumbnail](https://content.abcam.com/products/images/p40-phox-antibody-ep2142y-bsa-and-azide-free-ab247448--immunoprecipitation-img165207.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (AB247448)")
![Flow Cytometry (Intracellular) - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (AB247448), expandable thumbnail](https://content.abcam.com/products/images/p40-phox-antibody-ep2142y-bsa-and-azide-free-ab247448--flow-cytometry-intracellular-img67838.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (AB247448)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Version
Target data
Function
Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Alternative names
Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4, p40-phox, p40phox, NCF4, SH3PXD4
Recommended products
Rabbit Recombinant Monoclonal p40-phox antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4, p40-phox, p40phox, NCF4, SH3PXD4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP2142Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab247448 is the carrier-free version of Anti-p40-phox antibody [EP2142Y] ab76158.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
P40-phox also known as neutrophil cytosolic factor 4 (NCF4) is a regulatory component of the NADPH oxidase complex. It is expressed in phagocytic cells such as neutrophils and macrophages. The protein plays a role in the production of reactive oxygen species (ROS) during the respiratory burst. The molecular mass of p40-phox is approximately 40 kDa. It interacts with other subunits of the NADPH oxidase complex such as p67-phox and p47-phox contributing to the assembly and activation of the complex.
- Biological function summary
P40-phox participates in innate immune responses by regulating the NADPH oxidase complex's function. It acts as a scaffold to facilitate the interaction between cytosolic and membrane components. p40-phox integrates into the cytosolic fraction with other subunits and translocates to the membrane upon stimulation. This translocation is essential for generating superoxide radicals that are involved in defense against pathogens. Its functional domains enable it to bind phosphatidylinositol (34) bisphosphate which anchors the protein to the membrane. p40-phox itself is not an enzyme but modulates the activity of the complex.
- Pathways
The p40 protein serves as an important player in the signaling cascade of the respiratory burst. It interacts with the phosphoinositide 3-kinase (PI3K) pathway and cooperates with other proteins like p67-phox and p47-phox through phosphorylation-dependent mechanisms. The p40-phox is phox compatible meaning it can integrate into various signaling modules. Within the oxidative burst pathway it enhances the cytosolic complex's attachment to the membrane affecting the production of ROS.
- Associated diseases and disorders
Mutations in the p40-phox have been connected to chronic granulomatous disease (CGD) characterized by recurrent infections and granuloma formation. The lack of functional p40-phox disrupts the assembly of the NADPH oxidase complex leading to decreased ROS production. This deficiency impairs the ability of phagocytes to eliminate pathogens effectively. p40-phox is also related to inflammatory disorders like inflammatory bowel disease (IBD) as variations in the NCF4 gene affect the immune response. The dysfunction in ROS production impacts the signaling involving p67-phox and p47-phox further influencing disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448)
This data was developed using Anti-p40-phox antibody [EP2142Y] ab76158, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-p40-phox antibody [EP2142Y] (Anti-p40-phox antibody [EP2142Y] ab76158) at 1/50000 dilution
All lanes: HL60 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448), expandable thumbnail](https://content.abcam.com/products/images/p40-phox-antibody-ep2142y-bsa-and-azide-free-ab247448--immunocytochemistry-immunofluorescence-img131871.jpg "Immunocytochemistry/ Immunofluorescence - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448)")
Immunocytochemistry/ Immunofluorescence - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448)
This data was developed using Anti-p40-phox antibody [EP2142Y] ab76158, the same antibody clone in a different buffer formulation.ICC/IF image of Anti-p40-phox antibody [EP2142Y] ab76158 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-p40-phox antibody [EP2142Y] ab76158, 1/25 dilution) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448), expandable thumbnail](https://content.abcam.com/products/images/p40-phox-antibody-ep2142y-bsa-and-azide-free-ab247448--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137910.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448)
This data was developed using Anti-p40-phox antibody [EP2142Y] ab76158, the same antibody clone in a different buffer formulation.Anti-p40-phox antibody [EP2142Y] ab76158 at 1/100-1/250 dilution staining p40-phox in human spleen by Immunohistochemistry, Paraffin-embedded tissue.
![Immunoprecipitation - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448), expandable thumbnail](https://content.abcam.com/products/images/p40-phox-antibody-ep2142y-bsa-and-azide-free-ab247448--immunoprecipitation-img165207.jpg "Immunoprecipitation - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448)")
Immunoprecipitation - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448)
This data was developed using Anti-p40-phox antibody [EP2142Y] ab76158, the same antibody clone in a different buffer formulation.p40-phox was immunoprecipitated from 0.35 mg HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate 10 µg with Anti-p40-phox antibody [EP2142Y] ab76158 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate 10 µg
Lane 2: abab76158 IP in HL-60 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-p40-phox antibody [EP2142Y] ab76158 in HL-60 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-p40-phox antibody [EP2142Y] (Anti-p40-phox antibody [EP2142Y] ab76158)
Predicted band size: 39 kDa
Observed band size: 39 kDa
![Flow Cytometry (Intracellular) - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448), expandable thumbnail](https://content.abcam.com/products/images/p40-phox-antibody-ep2142y-bsa-and-azide-free-ab247448--flow-cytometry-intracellular-img67838.jpg "Flow Cytometry (Intracellular) - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448)")
Flow Cytometry (Intracellular) - Anti-p40-phox antibody [EP2142Y] - BSA and Azide free (ab247448)
This data was developed using Anti-p40-phox antibody [EP2142Y] ab76158, the same antibody clone in a different buffer formulation.Overlay histogram showing K562 cells stained with Anti-p40-phox antibody [EP2142Y] ab76158 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-p40-phox antibody [EP2142Y] ab76158, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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