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AB251710

Anti-p400 antibody

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(1 Publication)

Rabbit Polyclonal p400 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EP400 aa 900-1100.

View Alternative Names

CAGH32, KIAA1498, KIAA1818, TNRC12, EP400, E1A-binding protein p400, CAG repeat protein 32, Domino homolog, Trinucleotide repeat-containing gene 12 protein, p400 kDa SWI2/SNF2-related protein, hDomino

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p400 antibody (AB251710)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p400 antibody (AB251710)

Paraffin-embedded human cerebral cortex tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p400 antibody (AB251710)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p400 antibody (AB251710)

Paraffin-embedded human skin tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p400 antibody (AB251710)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p400 antibody (AB251710)

Paraffin-embedded human liver tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis. No nuclear positivity in hepatocytes.

Immunocytochemistry/ Immunofluorescence - Anti-p400 antibody (AB251710)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p400 antibody (AB251710)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for p400 (green) using ab251710 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p400 antibody (AB251710)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p400 antibody (AB251710)

Paraffin-embedded human testis tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EP400 aa 900-1100. The exact immunogen used to generate this antibody is proprietary information.

Q96L91

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p400 protein also known as EP400 plays a mechanical role as an ATPase in chromatin remodeling. Its molecular mass is approximately 420 kDa. This protein is predominantly expressed in tissues with high proliferative capacities such as testis and lymph nodes. It functions within the TIP60 complex also consisting of other important histone acetyltransferase components that regulate transcriptional activation and DNA repair processes.
Biological function summary

The p400 protein contributes significantly to gene expression and chromatin structure. It participates in the modification of chromatin architecture facilitating the access of transcription factors to DNA. p400 becomes an integral component of the TIP60 complex vital for the acetylation of nucleosomal histones which promotes transcriptional activation. Through these mechanisms p400 influences cellular processes such as cell cycle regulation and apoptosis.

Pathways

P400's functionality integrates into significant cellular pathways like the cell cycle and DNA repair pathways. Within the cell cycle p400's role in chromatin remodeling links it to the regulation of genes controlling cell proliferation. The protein interacts with histones and is part of complexes that include proteins such as TRRAP contributing to the DNA damage response and repair mechanisms. These connections highlight p400's involvement in cellular defense and maintenance of genomic stability.

P400's dysfunction associates with cancer and neurodegenerative disorders. Abnormal regulation of p400 due to its function in transcription and repair implicates its involvement in tumorigenesis particularly in cancers such as colorectal cancer. In neurological contexts aberrant p400 activity relates to neurodegenerative diseases like Alzheimer's where chromatin structure and gene expression go awry. The target’s interconnectedness with other proteins like TIP60 during the onset of these conditions highlights its importance in pathophysiological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.
See full target information EP400
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:5 PubMed39958879

2025

Relationship between transmembrane emp24 domain containing 2 expression and tumor stem cell characteristics in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhui Wu,Shunchu Zhang,Guimei Zou
View all publications
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