Rabbit Polyclonal p400 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human EP400.
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Publications
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- Cells 9:2020VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Moreno-Andrés et. al.PubMed 32708675
- Translational psychiatry 7:e10822017An interaction network of mental disorder proteins in neural stem cells.
- Molecular cell 61:27-382015EP400 Deposits H3.3 into Promoters and Enhancers during Gene Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuman K Pradhan,Trent Su,Linda Yen,Karine Jacquet,Chengyang Huang,Jacques Côté,Siavash K Kurdistani,Michael F CareyPubMed 26669263
- Molecular and cellular biology 34:3359-732014Cell activation-induced phosphoinositide 3-kinase alpha/beta dimerization regulates PTEN activity.
- PloS one 8:e541022013Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z.
- Cell 143:313-242010A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs.Applications:WBReactive species:MouseJonghwan Kim,Andrew J Woo,Jianlin Chu,Jonathan W Snow,Yuko Fujiwara,Chul Geun Kim,Alan B Cantor,Stuart H OrkinPubMed 20946988
- PLoS genetics 6:e10009832010The E1A-associated p400 protein modulates cell fate decisions by the regulation of ROS homeostasis.
- Nature cell biology 10:361-92008VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArthur P Young et. al.PubMed 18297059
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EP400. The exact immunogen used to generate this antibody is proprietary information. Database link Q96L91
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
Target data
Function
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.
Alternative names
CAGH32, KIAA1498, KIAA1818, TNRC12, EP400, E1A-binding protein p400, CAG repeat protein 32, Domino homolog, Trinucleotide repeat-containing gene 12 protein, p400 kDa SWI2/SNF2-related protein, hDomino
Recommended products
Rabbit Polyclonal p400 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human EP400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EP400. The exact immunogen used to generate this antibody is proprietary information. Database link Q96L91
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab70301 was affinity purified using an epitope specific to p400 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p400 protein also known as EP400 plays a mechanical role as an ATPase in chromatin remodeling. Its molecular mass is approximately 420 kDa. This protein is predominantly expressed in tissues with high proliferative capacities such as testis and lymph nodes. It functions within the TIP60 complex also consisting of other important histone acetyltransferase components that regulate transcriptional activation and DNA repair processes.
- Biological function summary
The p400 protein contributes significantly to gene expression and chromatin structure. It participates in the modification of chromatin architecture facilitating the access of transcription factors to DNA. p400 becomes an integral component of the TIP60 complex vital for the acetylation of nucleosomal histones which promotes transcriptional activation. Through these mechanisms p400 influences cellular processes such as cell cycle regulation and apoptosis.
- Pathways
P400's functionality integrates into significant cellular pathways like the cell cycle and DNA repair pathways. Within the cell cycle p400's role in chromatin remodeling links it to the regulation of genes controlling cell proliferation. The protein interacts with histones and is part of complexes that include proteins such as TRRAP contributing to the DNA damage response and repair mechanisms. These connections highlight p400's involvement in cellular defense and maintenance of genomic stability.
- Associated diseases and disorders
P400's dysfunction associates with cancer and neurodegenerative disorders. Abnormal regulation of p400 due to its function in transcription and repair implicates its involvement in tumorigenesis particularly in cancers such as colorectal cancer. In neurological contexts aberrant p400 activity relates to neurodegenerative diseases like Alzheimer's where chromatin structure and gene expression go awry. The target’s interconnectedness with other proteins like TIP60 during the onset of these conditions highlights its importance in pathophysiological processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-p400 antibody (ab70301)
All lanes: Western blot - Anti-p400 antibody (ab70301) at 0.4 µg/mL
All lanes: NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 343 kDa
Exposure time: 30s
Immunoprecipitation - Anti-p400 antibody (ab70301)
Immunoprecipitation of ab70301 using HeLa cells.
Lane 1: rabbit anti-p400 antibody
Lane 2: ab70301 current lot
Lane 3: ab70301 previous lot
Lane4: Control IgG
All lanes: Immunoprecipitation - Anti-p400 antibody (ab70301)
Predicted band size: 343 kDa
Exposure time: 3min
Western blot - Anti-p400 antibody (ab70301)
All lanes: Western blot - Anti-p400 antibody (ab70301) at 0.4 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: K-562 whole cell lysate at 50 µg
Lane 3: MCF-7 whole cell lysate at 50 µg
Predicted band size: 343 kDa
Exposure time: 30s
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