AB70301

Anti-p400 antibody

Name: Anti-p400 antibody (ab70301) | Abcam
Brand: Abcam Limited
SKU: AB70301
Price: 585 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(9 Publications)

Rabbit Polyclonal p400 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human EP400.

View Alternative Names

CAGH32, KIAA1498, KIAA1818, TNRC12, EP400, E1A-binding protein p400, CAG repeat protein 32, Domino homolog, Trinucleotide repeat-containing gene 12 protein, p400 kDa SWI2/SNF2-related protein, hDomino

3 Images

Supplier Data

Immunoprecipitation - Anti-p400 antibody (AB70301)

Immunoprecipitation of ab70301 using HeLa cells.

Lane 1 : rabbit anti-p400 antibody

Lane 2 : ab70301 current lot

Lane 3 : ab70301 previous lot

Lane4 : Control IgG

All lanes:

Immunoprecipitation - Anti-p400 antibody (ab70301)

Predicted band size: 343 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-p400 antibody (AB70301)

All lanes:

Western blot - Anti-p400 antibody (ab70301) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

K-562 whole cell lysate at 50 µg

Lane 3:

MCF-7 whole cell lysate at 50 µg

Predicted band size: 343 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-p400 antibody (AB70301)

All lanes:

Western blot - Anti-p400 antibody (ab70301) at 0.4 µg/mL

All lanes:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 343 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EP400. The exact immunogen used to generate this antibody is proprietary information.

Q96L91

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab70301 was affinity purified using an epitope specific to p400 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p400 protein also known as EP400 plays a mechanical role as an ATPase in chromatin remodeling. Its molecular mass is approximately 420 kDa. This protein is predominantly expressed in tissues with high proliferative capacities such as testis and lymph nodes. It functions within the TIP60 complex also consisting of other important histone acetyltransferase components that regulate transcriptional activation and DNA repair processes.

Biological function summary

The p400 protein contributes significantly to gene expression and chromatin structure. It participates in the modification of chromatin architecture facilitating the access of transcription factors to DNA. p400 becomes an integral component of the TIP60 complex vital for the acetylation of nucleosomal histones which promotes transcriptional activation. Through these mechanisms p400 influences cellular processes such as cell cycle regulation and apoptosis.

Pathways

P400's functionality integrates into significant cellular pathways like the cell cycle and DNA repair pathways. Within the cell cycle p400's role in chromatin remodeling links it to the regulation of genes controlling cell proliferation. The protein interacts with histones and is part of complexes that include proteins such as TRRAP contributing to the DNA damage response and repair mechanisms. These connections highlight p400's involvement in cellular defense and maintenance of genomic stability.

P400's dysfunction associates with cancer and neurodegenerative disorders. Abnormal regulation of p400 due to its function in transcription and repair implicates its involvement in tumorigenesis particularly in cancers such as colorectal cancer. In neurological contexts aberrant p400 activity relates to neurodegenerative diseases like Alzheimer's where chromatin structure and gene expression go awry. The target’s interconnectedness with other proteins like TIP60 during the onset of these conditions highlights its importance in pathophysiological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.

See full target information EP400

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell 186:5290-5307.e26 PubMed37922899

2023

Global identification of SWI/SNF targets reveals compensation by EP400.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin J E Martin,Eileen F Ablondi,Christine Goglia,Claudia A Mimoso,Piero R Espinel-Cabrera,Karen Adelman

Cells 9: PubMed32708675

2020

VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Moreno-Andrés,Hideki Yokoyama,Anja Scheufen,Guillaume Holzer,Hongqi Lue,Anna Katharina Schellhaus,Marion Weberruss,Masatoshi Takagi,Wolfram Antonin

Translational psychiatry 7:e1082 PubMed28375211

2017

An interaction network of mental disorder proteins in neural stem cells.

Applications

Species

Unspecified reactive species

M J Moen,H H H Adams,J H Brandsma,D H W Dekkers,U Akinci,S Karkampouna,M Quevedo,C E M Kockx,Z Ozgür,W F J van IJcken,J Demmers,R A Poot

Molecular cell 61:27-38 PubMed26669263

2015

EP400 Deposits H3.3 into Promoters and Enhancers during Gene Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Suman K Pradhan,Trent Su,Linda Yen,Karine Jacquet,Chengyang Huang,Jacques Côté,Siavash K Kurdistani,Michael F Carey

Molecular and cellular biology 34:3359-73 PubMed24958106

2014

Cell activation-induced phosphoinositide 3-kinase alpha/beta dimerization regulates PTEN activity.

Applications

Species

Mouse

Vicente Pérez-García,Javier Redondo-Muñoz,Amit Kumar,Ana C Carrera

PloS one 8:e54102 PubMed23349794

2013

Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z.

Applications

ChIP

Species

Human

Luca Bellucci,Mathieu Dalvai,Silvia Kocanova,Fatima Moutahir,Kerstin Bystricky

Cell 143:313-24 PubMed20946988

2010

A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs.

Applications

Species

Mouse

Jonghwan Kim,Andrew J Woo,Jianlin Chu,Jonathan W Snow,Yuko Fujiwara,Chul Geun Kim,Alan B Cantor,Stuart H Orkin

PLoS genetics 6:e1000983 PubMed20548951

2010

The E1A-associated p400 protein modulates cell fate decisions by the regulation of ROS homeostasis.

Applications

Species

Human

Lise Mattera,Céline Courilleau,Gaëlle Legube,Takeshi Ueda,Rikiro Fukunaga,Martine Chevillard-Briet,Yvan Canitrot,Fabrice Escaffit,Didier Trouche

Nature cell biology 10:361-9 PubMed18297059

2008

VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400.

Applications

Unspecified application

Species

Unspecified reactive species

Arthur P Young,Susanne Schlisio,Yoji Andrew Minamishima,Qing Zhang,Lianjie Li,Chiara Grisanzio,Sabina Signoretti,William G Kaelin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p400 antibody

Immunoprecipitation - Anti-p400 antibody (AB70301)

Western blot - Anti-p400 antibody (AB70301)

Western blot - Anti-p400 antibody (AB70301)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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